Journal
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
Volume 11, Issue -, Pages -Publisher
FRONTIERS MEDIA SA
DOI: 10.3389/fcimb.2021.806476
Keywords
skin microbiome; nanopore sequencing; MinION (TM); bacterial identification; skin mock community; 16S rRNA gene sequencing
Categories
Funding
- S-Biomedic
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This study used MinION(TM) nanopore sequencing technology to classify and analyze skin bacteria, finding that the existing protocols have biases in estimating the relative abundance of certain skin microbiome genera. By changing the amplification protocols, the accuracy of taxonomic classification for these three main skin bacterial genera can be improved. The study shows that MinION(TM) nanopore technology has great potential in studying skin microbiomes.
Human skin microbiome dysbiosis can have clinical consequences. Characterizing taxonomic composition of bacterial communities associated with skin disorders is important for dermatological advancement in both diagnosis and novel treatments. This study aims to analyze and improve the accuracy of taxonomic classification of skin bacteria with MinION (TM) nanopore sequencing using a defined skin mock community and a skin microbiome sample. We compared the Oxford Nanopore Technologies recommended procedures and concluded that their protocols highly bias the relative abundance of certain skin microbiome genera, most notably a large overrepresentation of Staphylococcus and underrepresentation of Cutibacterium and Corynebacterium. We demonstrated that changes in the amplification protocols improved the accuracy of the taxonomic classification for these three main skin bacterial genera. This study shows that MinION (TM) nanopore could be an efficient technology for full-length 16S rRNA sequencing; however, the analytical advantage is strongly influenced by the methodologies. The suggested alternatives in the sample processing improved characterization of a complex skin microbiome community using MinION (TM) nanopore sequencing.
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