Journal
INTERNATIONAL JOURNAL OF CANCER
Volume 139, Issue 8, Pages 1776-1787Publisher
WILEY-BLACKWELL
DOI: 10.1002/ijc.30234
Keywords
glioblastoma; glioblastoma stem-like cells; atypical protein kinase C iota; notch signaling
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Funding
- Helmholtz International Graduate School for Cancer Research
- Bundesministerium fur Bildung und Forschung, BMBF (NGFNplus) [01GS0883]
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In a previous study, Protein Kinase C iota (PRKCI) emerged as an important candidate gene for glioblastoma (GBM) stem-like cell (GSC) survival. Here, we show that PKCi is overexpressed and activated in patient derived GSCs compared with normal neural stem cells and normal brain lysate, and that silencing of PRKCI in GSCs causes apoptosis, along with loss of clonogenicity and reduced proliferation. Notably, PRKCI silencing reduces tumor growth in vivo in a xenograft mouse model. PKCi has been intensively studied as a therapeutic target in non-small cell lung cancer, resulting in the identification of an inhibitor, aurothiomalate (ATM), which disrupts the PKCi/ERK signaling axis. However, we show that, although sensitive to pharmacological inhibition via a pseudosubstrate peptide inhibitor, GSCs are much less sensitive to ATM, suggesting that PKCi acts along a different signaling axis in GSCs. Gene expression profiling of PRKCI-silenced GSCs revealed a novel role of the Notch signaling pathway in PKCi mediated GSC survival. A proximity ligation assay showed that Notch1 and PKCi are in close proximity in GSCs. Targeting PKCi in the context of Notch signaling could be an effective way of attacking the GSC population in GBM.
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