Journal
CLINICAL EPIGENETICS
Volume 14, Issue 1, Pages -Publisher
BMC
DOI: 10.1186/s13148-022-01245-3
Keywords
Cell-free DNA; cfDNA; Methylation; Tissue of origin; cfDNA release; Exercise; Physical activity; Hematological malignancies
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Funding
- Johannes Gutenberg University Mainz
- German Ministry of Education and Research [03VP06120]
- German Research Foundation [CRC156/2 KS01, CRC1066/3 TP B18, CRC1292/2 TP21N]
- Projekt DEAL
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Physical activity leads to an increase in cell-free DNA, with the majority of this DNA originating from granulocytes, which could compromise the accuracy of liquid biopsy.
Physical activity impacts immune homeostasis and leads to rapid and marked increase in cell-free DNA (cfDNA). However, the origin of cfDNA during exercise remains elusive and it is unknown if physical activity could improve or interfere with methylation based liquid biopsy. We analyzed the methylation levels of four validated CpGs representing cfDNA from granulocytes, lymphocytes, monocytes, and non-hematopoietic cells, in healthy individuals in response to exercise, and in patients with hematological malignancies under resting conditions. The analysis revealed that physical activity almost exclusively triggered DNA release from granulocytes, highlighting the relevance as a pre-analytical variable which could compromise diagnostic accuracy.
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