Article
Biochemistry & Molecular Biology
Avinash Mishra, Anurag S. Rathore
Summary: The study conducted computational analysis on the binding of Remdesivir and Galidesivir with SARS-CoV2 RdRp, revealing similar binding energies but distinct binding locations compared to natural substrate ATP. Simulation results indicated that Galidesivir binds at a non-catalytic location with weaker binding strength, while Remdesivir binds at the catalytic site showing potent inhibitory effects on RdRp function. Additionally, the study examined the binding of co-factor units nsp7 and nsp8 with RdRp complexed with Remdesivir and Galidesivir over a 100 ns simulation trajectory.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Multidisciplinary Sciences
Xianjin Zhou
Summary: A simple and rapid one-step test was developed to detect antibodies against the SARS-CoV2 N nucleocapsid protein. The test demonstrated superior sensitivity and specificity, providing a universal method for tracking virus infection in both humans and animal reservoirs.
Article
Biology
David W. Hawman, Kimberly Meade-White, Jacob Archer, Shanna S. Leventhal, Drew Wilson, Carl Shaia, Samantha Randall, Amit P. Khandhar, Kyle Krieger, Tien-Ying Hsiang, Michael Gale, Peter Berglund, Deborah Heydenburg Fuller, Heinz Feldmann, Jesse H. Erasmus
Summary: Despite efforts to control the SARS-CoV2 pandemic, the virus continues to spread with the emergence of new variants. Existing vaccines may have reduced effectiveness against these variants, leading to the development of second-generation vaccines. A study evaluated an RNA vaccine that can target multiple variants and showed promising results in mice and hamsters.
Article
Anatomy & Morphology
Gerard J. Nuovo, David Suster, Esmerina Tili, Hamdy Awad, Cynthia Magro
Summary: This manuscript presents a stringent protocol for in situ detection of SARS-CoV-2 RNA and viral proteins. The key aspects of the protocol include serial section analysis, observation of cytologic alterations, demonstration of host response, co-labeling experiments, and optimization conditions. The experimental results reveal the distribution of SARS-CoV-2 in macrophages and endothelial cells in the lung, as well as the presence of viral capsid proteins in multiple organs.
APPLIED IMMUNOHISTOCHEMISTRY & MOLECULAR MORPHOLOGY
(2022)
Article
Chemistry, Multidisciplinary
Lukasz Suprewicz, Maxx Swoger, Sarthak Gupta, Ewelina Piktel, Fitzroy J. Byfield, Daniel Iwamoto, Danielle Germann, Joanna Reszec, Natalia Marcinczyk, Robert J. Carroll, Marzena Lenart, Krzysztof Pyre, Paul Janmey, J. M. Schwarz, Robert Bucki, Alison Patteson
Summary: The study suggests that vimentin may act as an additional co-receptor for the SARS-CoV-2 spike protein, facilitating cell entry alongside ACE2.
Review
Medicine, Research & Experimental
Reshu Saxena, Priyanka Sharma, Sandeep Kumar, Niteshkumar Agrawal, Sumit Kumar Sharma, Amit Awasthi
Summary: Mitochondria are dynamic cellular organelles that play diverse roles in cellular functions. Viral proteins specifically target mitochondria to subvert host defense and facilitate viral infection. By manipulating mitochondrial dynamics and functions, viruses are able to control host cell apoptosis, metabolic state, and evade immune responses, ultimately achieving persistent infection.
Article
Biochemistry & Molecular Biology
Willie Elliott, Maheedhara R. Guda, Swapna Asuthkar, Narasaraju Teluguakula, Durbaka V. R. Prasad, Andrew J. Tsung, Kiran K. Velpula
Summary: The evolution of variants of SARS-CoV-2 presents challenges to vaccine efficacy, necessitating the development of pharmacotherapeutics specific to the pathophysiology of the virus. Peptidylarginine deiminases (PADs) and their inhibitors may play a crucial role in reducing thrombotic complications in COVID-19 patients.
Article
Biophysics
Mohd Azhar, Rhythm Phutela, Manoj Kumar, Asgar Hussain Ansari, Riya Rauthan, Sneha Gulati, Namrata Sharma, Dipanjali Sinha, Saumya Sharma, Sunaina Singh, Sundaram Acharya, Sajal Sarkar, Deepanjan Paul, Poorti Kathpalia, Meghali Aich, Paras Sehgal, Gyan Ranjan, Rahul C. Bhoyar, Khushboo Singhal, Harsha Lad, Pradeep Kumar Patra, Govind Makharia, Giriraj Ratan Chandak, Bala Pesala, Debojyoti Chakraborty, Souvik Maiti
Summary: FELUDA is a new rapid detection method for DNA/RNA pathogenic sequences or variants, which can accurately detect single nucleotide variants (SNVs) including heterozygous carriers with 100% accuracy. It is semi-quantitative, adaptable to multiple signal detection platforms, and can be used for various applications such as molecular diagnosis during infectious disease outbreaks like COVID-19.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Infectious Diseases
Jorge Hernandez Lopez, Alvaro Santos Romo, Daniel Coronado Molina, Gerardo Alvarez Hernandez, Angel Benjamin Gutierrez Cureno, Magali Aviles Acosta, Carlos Andres Aviles Gaxiola, Marcos Jose Serrato Felix, Teresa Gollas Galvan
Summary: The study successfully detected the SARS-CoV-2 virus using a 0.22 mm pore filter, indicating the presence of the virus in contaminated areas in trapped particulate matter near patients.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Biology
Zaigham Abbas Rizvi, Rajdeep Dalal, Srikanth Sadhu, Akshay Binayke, Jyotsna Dandotiya, Yashwant Kumar, Tripti Shrivastava, Sonu Kumar Gupta, Suruchi Aggarwal, Manas Ranjan Tripathy, Deepak Kumar Rathore, Amit Kumar Yadav, Guruprasad R. Medigeshi, Amit Kumar Pandey, Sweety Samal, Shailendra Asthana, Amit Awasthi
Summary: Using a hamster model, researchers found that early SARS-CoV-2 infection leads to acute inflammatory response and lung pathology, while late-stage infection causes cardiovascular complications characterized by ventricular wall thickening and interstitial coronary fibrosis. Molecular profiling showed elevated levels of cardiac troponin I, cholesterol, low-density lipoprotein, and long-chain fatty acid triglycerides in the infected hamsters' serum. Metabolomics profiling identified N-acetylneuraminate as a common metabolic marker between infected hamsters and COVID-19 patients. The study suggests that hamsters are a suitable animal model for studying cardiovascular complications associated with post-COVID sequelae.
Article
Environmental Sciences
Nallin Sharma, Chia-Hung Chi, Deepak Dabur, Andrew Chi-Chang Tsai, Hui-Fen Wu
Summary: This article discusses the association between SARS-CoV2 and ACE2, and the relation of ACE I/D polymorphism with the spread of the virus. It also presents the synthesis of a unique SnO2-xNx tpod nanostructure, which efficiently attaches to both ACE1 and ACE2, and can be used for detecting SARS-CoV2.
ENVIRONMENTAL RESEARCH
(2023)
Article
Acoustics
Jorge Alvarez-Troncoso, Luis Ramos-Ruperto, Pelayo Fernandez-Cidon, Elena Trigo-Esteban, Yale Tung-Chen, Carmen Busca-Arenzana, Manuel Quintana-Diaz, Antonio Buno-Soto, Francisco Arnalich-Fernandez, Carmen Fernandez-Capitan
Summary: In this study, 26.5% of COVID-19 patients were found to have VTD, with age, D-dimer levels, and detection of DVT and PE being associated with patient mortality. Screening based on age-adjusted D-dimer and POCUS showed high sensitivity and specificity in detecting VTD, providing valuable insights for early intervention in COVID-19 patients.
JOURNAL OF ULTRASOUND IN MEDICINE
(2022)
Article
Immunology
Christian Herr, Sebastian Mang, Bahareh Mozafari, Katharina Guenther, Thimoteus Speer, Martina Seibert, Sanjay Kumar Srikakulam, Christoph Beisswenger, Felix Ritzmann, Andreas Keller, Rolf Mueller, Sigrun Smola, Dominic Eisinger, Michael Zemlin, Guy Danziger, Thomas Volk, Sabrina Hoersch, Marcin Krawczyk, Frank Lammert, Thomas Adams, Gudrun VVagenpfeil, Michael Kindermann, Constantin Marcu, Zuhair Wolf Dietrich Ataya, Marc Mittag, Konrad Schwarzkopf, Florian Custodis, Daniel Grandt, Harald Schaefer, Kai Eltges, Philipp M. Lepper, Robert Bals
Summary: This study evaluated the biomarkers in patients with COVID-19, pneumonia, and control groups, identifying specific proteins associated with disease severity and mortality. Different models were developed to predict mortality based on baseline measurements of protein markers. The use of established assay technologies allows for rapid translation into clinical practice.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Article
Multidisciplinary Sciences
Gijsbert J. Jansen, Marit Wiersma, Willem J. B. van Wamel, Inge D. Wijnberg
Summary: A new method using saliva samples for detecting SARS-CoV-2 can distinguish between the presence and activity of the virus, potentially providing valuable information in the qualitative results of the virus.
Article
Medicine, General & Internal
Maria N. Rayo, Irene Fernandez-Buhigas, Emilia Ferrer, Maria Arrebola, Maria M. Gil, Belen Santacruz
Summary: This study evaluated the clinical implementation of a preventive COVID-19 protocol in an obstetric outpatient service, focusing on the re-organization of appointments and documented infections among healthcare workers. The results showed that the implementation of the preventive protocol reduced the percentage of healthcare workers affected by SARS-CoV2.
FRONTIERS IN MEDICINE
(2022)