4.7 Article

Microbiota associated with the large-scale outdoor cultivation of the cyanobacterium Synechococcus sp. PCC 7002

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ELSEVIER
DOI: 10.1016/j.algal.2021.102382

Keywords

Synechococcus; Cyanobacteria; Mass cultivation; Photobioreactor; Open-raceway pond; Predation

Funding

  1. U.S. Department of Energy Office of Energy Efficiency [DE-EE0005996]
  2. Air Force Office of Scientific Research
  3. AFOSR [95501410147]

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The study aimed to measure the outdoor productivity of the euryhaline cyanobacterium Synechococcus sp. PCC 7002, with promising results in flat-panel photobioreactors but challenges in open raceway ponds. Potential competitors and predators were identified through microbiota analysis, highlighting the importance of developing crop protection strategies for mass cultivation in open pond systems.
The model euryhaline cyanobacterium Synechococcus sp. PCC 7002 has potential as a platform for biotechnological applications because of its genetic amenability and fast growth rates. The purpose of this work was to measure the outdoor productivity metrics of Synechococcus sp. PCC 7002, which we tested by cultivation in 110 L flat-panel photobioreactors and 1025 L open raceway ponds at the Algae Testbed Public Private Partnership (ATP3) algae culturing testbed site at the Arizona Center for Algae Technology and Innovation (AzCATI). This work was carried out during the summer growing season when ambient temperatures and light availability are highest to support maximum photoautotrophic biomass yields. Modifications to the traditional A+ laboratory growth media for Synechococcus sp. PCC 7002 included the use of urea as a nitrogen source, and CO2 sparging for automated pH control in open ponds. Synechococcus showed robust growth in flat-panel photobioreactors, which are suited for the containment of modified organisms; however, cultivation in the open-raceway pond system was challenging due to pond crashes. We investigated the microbiota associated with Synechococcus pond cultures using Illumina amplicon sequencing of the 16S rRNA genes of bacteria and archaea and the 18S rRNA genes of microbial eukaryotes, to identify potential competitor and predator genera. These results pointed to likely predation of Synechococcus by a ciliated protozoan of the genus Euplotes. Development of crop protection strategies will be crucial for enabling the mass cultivation of Synechococcus in open pond systems. Superscript/Subscript Available

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