Journal
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
Volume 93, Issue -, Pages 1410-1419Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.ijbiomac.2016.05.024
Keywords
Cryogel; Hyaluronic acid; Chondroitin sulfate; Cartilage tissue engineering; Poly (ethylene glycol) diacrylates
Funding
- Ministry of Health and Welfare of Korea [HI13C1789]
- International Research & Development Program of the National Research Foundation of Korea (NRF) - Ministry of Science, ICT & Future Planning [2014K1A3A1A21001365]
- National Research Foundation of Korea [2015H1A2A1029321, 2014K1A3A1A21001365] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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In this study, we investigated various highly porous extracellular matrix (ECM)-based cryogels for cartilage tissue engineering. For the fabrication of ECM-based cryogels, either methacrylated chondroitin sulfate (MeCS) or methacrylated hyaluronic acid (MeHA) were cross-linked along with poly (ethylene glycol) diacrylates (PEGDA) via free radical polymerization under freezing conditions. This procedure induces ice crystallization (used as a porogen) prior polymer crosslinking in which, after complete cryopolymerization, a thawing process transforms the ice crystals into a unique interconnected macroporous structure within ECM-cryogels. The developed ECM-cryogels exhibited an average macroporosity of 75% and supported the infiltration of chondrocyteds. When rabbit chondrocytes were cultured on ECM-cryogels, MeCS-based cryogels stimulated aggrecan gene expression and GAG accumulation, whereas MeHA-based cryogels stimulated type II collagen gene expression and collagen accumulation. These results demonstrate that design of ECM-based cryogels can play an important role in promoting specific ECM proteins secretion for cartilage tissue engineering. (C) 2016 Elsevier B.V. All rights reserved.
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