Article
Biotechnology & Applied Microbiology
Harshada M. Salvi, Ganapati D. Yadav
Summary: Epoxide hydrolases are enzymes widely found in nature that catalyze the hydrolysis of epoxides to diols without the need for a cofactor. A method for immobilization of epoxide hydrolase based on biomimetic mineralization has been reported, resulting in organic-inorganic hybrid nanoflowers with higher catalytic activity and stability. The nanoflowers, consisting of enzyme epoxide hydrolase and Ca2+ ions, exhibit a unique hierarchical flower-like structure and have been applied for styrene oxide hydrolysis. Factors affecting morphology, catalytic activity, and stability were studied to understand the activity recovery of the synthesized hybrid nanoflowers.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Engineering, Environmental
Miaorong Zhang, Yan Zhang, Chuankai Yang, Chunyun Ma, Jianguo Tang
Summary: Enzyme-inorganic hybrid nanoflowers (HNFs) are a novel hybrid nanomaterial with superior performances, involving classification, synthesis, functionalization, and various potential application areas. The focus of the review is on developing classification, synthesis methods and applications of enzyme-inorganic HNFs in the field of biocatalysis. Current problems and prospects for further study are proposed.
CHEMICAL ENGINEERING JOURNAL
(2021)
Review
Chemistry, Inorganic & Nuclear
Jianxiong Chen, Zitao Guo, Yu Xin, Zhenghua Gu, Liang Zhang, Xuan Guo
Summary: This review article focuses on the classification, structure-function relationship, and applications of organic-inorganic hybrid nanoflowers (HNFs) in areas such as biocatalysis, biosensing, and medical diagnosis. The authors summarize the strategy for classifying HNFs based on their organic components and discuss the influence of the synthetic process on the properties of HNFs. They also explore the development of functionalized HNFs and their applications in industrial biocatalysis, environmental remediation, antibacterial properties, and biosensing. The potential of HNF-based biosensors for medical disease diagnosis and prevention is highlighted. The article concludes with an overview of the challenges and prospects for the further development of efficient and multi-functional HNF-based biocatalysts.
COORDINATION CHEMISTRY REVIEWS
(2023)
Article
Chemistry, Physical
Shubhankar Dube, Deepak Rawtani
Summary: Organic-inorganic hybrid nanoflowers serve as a novel class of nanostructures with superior activity and reusability, acting as a host platform for immobilizing biomolecules and exhibiting enzyme mimicking activity in many cases. However, the facile synthesis involves intricacies that require in-depth analysis to fully realize its potential as an immobilization technique.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2021)
Article
Engineering, Chemical
Chen Gao, Bright Appiah, Zhi-Cheng Zou, Bo-Wei Zhang, Jin-Hui Zhou, Chen Yu, Ling-Ling Li, Can Quan, Zhong-Hua Yang
Summary: Organic-inorganic hybrid nanoflowers (hNFs) have attracted extensive attention as a novel enzyme immobilization mode due to their unique high specific surface area and distinctive microstructure. In this study, a NP1-inorganic hNF (NP1-hNF) was prepared by self-assembly co-precipitation method using CaCl2 and BSA. The NP1-hNFs showed significantly enhanced catalytic activity and improved thermal stability compared to the free enzyme. The NP1-hNFs were further entrapped in calcium alginate microbeads for improved reusability, retaining 65% of residual activity after five recycle uses and 96% after 50 days of storage.
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2023)
Article
Chemistry, Physical
Hossein Jafari-Nodoushan, Somayeh Mojtabavi, Mohammad Ali Faramarzi, Nasrin Samadi
Summary: Organic-inorganic hybrid nanoflowers (HNFs) are hierarchical flower-shaped microstructures composed of organic and inorganic constituents. They have a high surface-to-volume ratio and are mainly used for efficient biocatalysis and highly sensitive biosensing. They also have other important applications. However, there are still unknown aspects and future directions of research in this field.
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2022)
Review
Biotechnology & Applied Microbiology
Zichen Wang, Ruirui Wang, Zixin Geng, Xiuyan Luo, Jiahui Jia, Saizhao Pang, Xianwei Fan, Muhammad Bilal, Jiandong Cui
Summary: Hybrid nanomaterials have emerged as a new interface of nanobiocatalysis, with inorganic crystal nanoflowers and metal-organic frameworks (MOFs) being of particular interest for enzyme immobilization. However, there is a lack of literature reviews on enzyme@MOF and enzyme-hybrid nanoflower composites and their synthesis strategies and applications in biotechnology. This review discusses innovative synthetic strategies and biotechnological applications of enzyme@MOF and enzyme-hybrid nanoflower composites, aiming to provide insights and ideas for future research.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Multidisciplinary Sciences
Khashayar Vojdanitalab, Hossein Jafari-Nodoushan, Somayeh Mojtabavi, Mahtab Shokri, Hoda Jahandar, Mohammad Ali Faramarzi
Summary: A novel concentrated method was developed for the instant fabrication of laccase@Co-3(PO4)(2)center dot hybrid nanoflowers. The synthesized nanoflowers exhibited excellent catalytic efficiency and stability, making them promising biocatalysts and pollutant removal agents.
SCIENTIFIC REPORTS
(2022)
Article
Biotechnology & Applied Microbiology
Kotchakorn T. Sriwong, Kazuki Ogura, Muhammad Arisyi Hawari, Tomoko Matsuda
Summary: In this study, immobilization of Geotrichum candidum aldehyde dehydrogenase (GcALDH) was achieved through organic-inorganic nanocrystal formation, which resulted in improved stability and recyclability. The GcALDH nanocrystal exhibited high activity, improved thermostability and substrate promiscuity, indicating its potential use in green industries. This is the first report of ALDH-inorganic nanocrystal formation with superior properties.
ENZYME AND MICROBIAL TECHNOLOGY
(2021)
Article
Green & Sustainable Science & Technology
Yang Liu, Ziyuan Wang, Yuxiao Feng, Yi Jiao, Le Zhong, Geling Kuang, Yingjie Du, Muhammad Bilal, Shiru Jia, Jiandong Cui
Summary: Isoorotic acid-zinc phosphate hybrid nanoflowers (IA-ZP NF) using zinc (II) ions as the inorganic component and isoorotic acid as the organic component have been developed as a new kind of antibacterial material. The IA-ZP NF exhibit enhanced antibacterial activity and low toxicity due to the synergistic effects of zinc (II) release, generation of reactive oxygen species (ROS), and membrane interaction.
SUSTAINABLE MATERIALS AND TECHNOLOGIES
(2022)
Review
Biotechnology & Applied Microbiology
Pravin D. Patil, Radhika K. Kelkar, Neha P. Patil, Pradnya V. Pise, Sadhana P. Patil, Arundhatti S. Patil, Nishant S. Kulkarni, Manishkumar S. Tiwari, Ajay N. Phirke, Shamraja S. Nadar
Summary: The use of organic-inorganic hybrid nanoflowers as support material for enzyme immobilization has become popular due to their stability, ease of preparation, and enhanced catalytic activity. However, their low density and high dispersion make them difficult to handle and separate. Magnetic nanoflowers have emerged as a promising alternative platform for enzyme immobilization due to their easy separation, structural stability, and ability to enhance catalytic efficiency. This review focuses on different methods for designing magnetic nanoflowers, examples of enzymes immobilized in magnetic nanoflowers, their applications, and ways to improve material properties for enhanced catalytic activity, structural stability, and scalability.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Review
Materials Science, Biomaterials
Xiao Liang, Yong Liu, Kai Wen, Wei Jiang, Quanshun Li
Summary: Enzyme immobilization in inorganic hybrid nanoflowers is a biomimetic mineralization method with mild reaction conditions, which helps to obtain ideal biocatalysts. Immobilized enzymes in inorganic hybrid nanoflowers have been successfully used in biocatalysis, producing medical intermediates and biodiesel, as well as in developing biosensors for detecting toxic substances and disease biomarkers.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Engineering, Chemical
Fatemeh Ahmadpoor, Mahmoud Nasrollahzadeh, Mahsa Mohammad
Summary: This study successfully designed an efficient and green hierarchical copper phosphate-lignosulfonate nanoflowers using an eco-friendly self-assembly method for catalytic reduction of organic dyes in water. Lignosulfonate played a critical role in controlling the size and morphology of Cu-LS nanoflowers, which showed high stability and reusability in catalytic tests.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Review
Chemistry, Multidisciplinary
Felipe Pereira da Costa, Eliane Pereira Cipolatti, Agenor Furigo Junior, Rosana Oliveira Henriques
Summary: Enzymes are biocatalysts known for their versatility, selectivity, and favorable operating conditions compared to chemical catalysts. However, their large-scale application is limited by high costs and low stability under extreme reaction conditions. Immobilization techniques can solve these problems, and organic-inorganic hybrid nanoflowers have shown advantages such as high surface area, simplicity of synthesis, high stability, and catalytic activity in enzymatic catalysis.
Article
Biochemistry & Molecular Biology
Mostafa Mostafavi, Mahtab Beihaghi Poor, Zohreh Habibi, Mehdi Mohammadi, Maryam Yousefi
Summary: In this study, 3D flower-like structured organic-inorganic hybrid nanoflowers (hNFs) with various lipases and graphene quantum dots (GQDs) were prepared and characterized. The addition of GQDs significantly increased the activity of the lipase hNFs. The lipase@GQDs hNFs showed higher activity compared to free lipase and other hNFs without GQDs, and also exhibited improved pH and thermal stability.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Manish Salgaonkar, Shamraja S. Nadar, Virendra K. Rathod
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2018)
Article
Biochemistry & Molecular Biology
Shamraja S. Nadar, Virendra K. Rathod
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2019)
Article
Engineering, Chemical
Sarita D. Gawas, Nishat Khan, Virendra K. Rathod
BRAZILIAN JOURNAL OF CHEMICAL ENGINEERING
(2019)
Review
Chemistry, Inorganic & Nuclear
Shamraja S. Nadar, Leena Vaidya, Shefali Maurya, Virendra K. Rathod
COORDINATION CHEMISTRY REVIEWS
(2019)
Article
Biochemistry & Molecular Biology
Shamraja S. Nadar, Virendra K. Rathod
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Article
Biochemistry & Molecular Biology
Leena B. Vaidya, Shamraja S. Nadar, Virendra K. Rathod
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Review
Biochemistry & Molecular Biology
Shamraja S. Nadar, Leena Vaidya, Virendra K. Rathod
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2020)
Article
Biotechnology & Applied Microbiology
Shefali S. Maurya, Shamraja S. Nadar, Virendra K. Rathod
JOURNAL OF BIOTECHNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Shefali S. Maurya, Shamraja S. Nadar, Virendra K. Rathod
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2020)
Article
Biophysics
Leena B. Vaidya, Shamraja S. Nadar, Virendra K. Rathod
COLLOIDS AND SURFACES B-BIOINTERFACES
(2020)
Review
Food Science & Technology
Shamraja S. Nadar, Pravin D. Patil, Nanda M. Rohra
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2020)
Article
Biotechnology & Applied Microbiology
Shamraja S. Nadar, Virendra K. Rathod
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2019)
Article
Engineering, Environmental
Manish Salgaonkar, Shamraja S. Nadar, Virendra K. Rathod
JOURNAL OF ENVIRONMENTAL CHEMICAL ENGINEERING
(2019)
Article
Biotechnology & Applied Microbiology
Shamraja S. Nadar, Virendra K. Rathod
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2019)
Article
Biotechnology & Applied Microbiology
Nishat R. Khan, Sarita D. Gawas, Virendra K. Rathod
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2018)
Article
Biochemistry & Molecular Biology
Wei-Han Hui, Yen-Lin Chen, Shu-Wei Chang
Summary: Crosslinking affects the mechanical behavior and tissue metabolism of collagen-based tissues. Aging and diabetes alter the crosslinking type and density, thus changing tissue properties. High-connectivity enzymatic crosslinking and advanced-glycation end-products result in uniform deformation under daily activity, while low-connectivity enzymatic crosslinking does not. High-connectivity model displays more sliding, while AGEs induce instability in structures near the binding sites.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yao-Bin Ma, Xiao-Qiu Zhou, Wei-Dan Jiang, Pei Wu, Yang Liu, Shu-Wei Li, Ling Tang, Lu Zhang, Hai-Feng Mi, Lin Feng
Summary: Tea polyphenols (TPs) are an inexpensive feed additive that can protect the gill function of fish against Flavobacterium columnare (F. columnare) through various mechanisms, including suppression of oxidative stress, apoptosis, and inflammation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Yan-Jia Guo, Jing-Ru Pang, Yu Zhang, Zhong-Rui Li, Xiao-Lin Zi, Hong-Min Liu, Ning Wang, Li-Juan Zhao, Ya Gao, Bo Wang, Piet Herdewijn, Cheng-Yun Jin, Ying Liu, Yi-Chao Zheng
Summary: This study identifies the conjugation of NEDD8 to LSD1 and its impact on GC cells, revealing the significance of LSD1 neddylation in GC.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Geum-Jae Jeong, Fazlurrahman Khan, Nazia Tabassum, Young-Mog Kim
Summary: Vibrio species are motile gram-negative bacteria commonly found in aquatic environments. They can adapt to environmental fluctuations and develop protective mechanisms against host immune systems. Different types of sigma and anti-sigma factors play important roles in regulating gene expression and virulence in Vibrio species.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Zahra Mohammadbagheri, Behrooz Movahedi, Shaghayegh Saeedi, Abbas Rahmati
Summary: In this study, a reusable biobased composite hydrogel was successfully synthesized and used for the removal of Tl(I) from aqueous solutions. The hydrogel exhibited high adsorption capacity and thermal stability, and the adsorption process was found to be thermodynamically spontaneous. The results demonstrated that the hydrogel can be reused multiple times without significant loss of its adsorption capacity.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Iqra Batool, Muhammad Imran, Ayesha Anwar, Farhan Ahmed Khan, Afrah E. Mohammed, Ashwag Shami, Hafiz Iqbal
Summary: Enzyme immobilization on ZnO/SnO2 and ZnO/SnO2/SA nanocomposites was studied, and the catalytic and biochemical characteristics of the immobilized enzyme were investigated. The results showed that ZnO/SnO2 and ZnO/SnO2/SA nanostructures are suitable carriers for enzyme immobilization.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Elias Mosaffa, Dhruvi Patel, Nasim Amiri Ramsheh, Rishikumar Indravadan Patel, Atanu Banerjee, Hossein Ghafuri
Summary: In this study, a unique 3D bead structure of adsorbent, composed of bacterial cellulose microfilaments reinforced chitosan adorned with melamine 2D plates, was synthesized for anionic dye removal. The adsorbent showed high specific surface area and abundant functional groups, resulting in exceptional adsorption capacity. The characteristics and optimal conditions of the adsorbent were assessed and analyzed.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Carolina Gismene, Jorge Enrique Hernandez Gonzalez, Marilia de Freitas Calmon, Andrey Fabricio Ziem Nascimento, Angela Rocio Nino Santisteban, Felipe Antunes Calil, Alana Della Torre da Silva, Paula Rahal, Rejane Maira Goes, Raghuvir Krishnaswamy Arni, Ricardo Barros Mariutti
Summary: Mammaliicoccus sciuri, a bacterium of clinical and veterinary relevance, expresses the virulence factor Exfoliative Toxin C (ExhC), which binds specifically to desmoglein-1 (Dsg1) and plays a role in tissue integrity. This study investigates the necrotic activity of ExhC and identifies a variant lacking necrotic potential but retaining enzymatic activity through mutagenesis experiments and crystal structure determination.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Bibhas K. Bhunia, Ashutosh Bandyopadhyay, Souradeep Dey, Biman B. Mandal
Summary: Intervertebral disc degeneration is a major cause of chronic low back pain and disability. Researchers have developed a bioactive silk-based hybrid hydrogel system functionalized with decellularized human Wharton's jelly extracellular matrix as bioactive cues. The hydrogels mimic the rheological and mechanical properties of the native nucleus pulposus tissue and support cell viability, proliferation, and tissue maturation.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Qun Zhang, Yonggan Yan, Zhao Li, Jing Du, Kai Zhang, Liguo Zhang, Ting Wang, Alberto Bianco, Shaohua Ge, Baojin Ma
Summary: A new crosslinking strategy has been devised to construct injectable sodium alginate hydrogels, which can be regulated for injectability and have great potential in biomedical applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Seong Eun Hong, Ji-Soo Lee, Hyeon Gyu Lee
Summary: The potential of multi-layer nanoemulsions for improving the cellular uptake, antioxidant activity, and in vitro bioaccessibility of alpha-tocopherol was examined. The results showed that the multi-layer nanoemulsions significantly enhanced the cellular uptake and antioxidant activity of alpha-tocopherol compared to free coumarin 6. Furthermore, the use of chitosan and dextran sulfate as wall materials in the multi-layer nanoemulsions led to even higher cellular uptake, in vitro bioaccessibility, and antioxidant activity of alpha-tocopherol.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jingjing Yu, Yanhui Zhang, Ruoning Zhang, Yanxiang Gao, Like Mao
Summary: High internal phase emulsions (HIPEs) based on beeswax (BW) oleogels and octenyl succinic acid starch (OSA starch) were prepared by a simple one-step method. The results showed that oleogelation improved the stability and encapsulation capability of the HIPEs, which is significant for the development of novel healthy food.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Bi-Feng Yao, Xiu-Ju Luo, Jun Peng
Summary: The balanced and continuous fission and fusion of mitochondria, regulated by OPA1, are important for maintaining mitochondrial dynamics in cardiovascular disorders. Understanding the role of OPA1 in mitochondrial fusion can provide insights into the pathogenesis of cardiovascular diseases.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Review
Biochemistry & Molecular Biology
Gokul Paramasivam, Anandhavelu Sanmugam, Vishnu Vardhan Palem, Murugan Sevanan, Ananda Babu Sairam, Nachiappan Nachiappan, Buhyun Youn, Jung Sub Lee, Muthuchamy Nallal, Kang Hyun Park
Summary: Nanomaterials are being used to deliver therapeutic agents in living systems. Different nanotechnological applications such as immunosensors, immune assays, and drug delivery are being implemented using nanotechnology. Genomics plays a crucial role in selecting appropriate drugs for personalized cancer therapy. It helps in analyzing gene and protein expression, disease classification, and designing disease models. Delivering drugs to specific areas using nanoparticles is a challenging issue.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)
Article
Biochemistry & Molecular Biology
Jing Li, Wei Wang, Han Wu, Feng Peng, Hui Gao, Ying Guan
Summary: In this study, CMH-based CPF films were prepared and their thermal and barrier properties were successfully regulated. The tensile strength and water vapor barrier property of CPF films were significantly improved, making them promising for food packaging applications.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2024)