Review
Biology
Elena V. Sazonova, Svetlana V. Petrichuk, Gelina S. Kopeina, Boris Zhivotovsky
Summary: Mitotic catastrophe, a cell death mechanism linked to delayed mitosis, may result in apoptosis, necrosis, or autophagy depending on molecular perturbations. Associated with cell aging and tumor elimination, effective triggering in clinical practice requires induction of DNA damage and inhibition of molecular pathways.
Article
Cell Biology
Xin Li, Zhiguo Niu, Chen Sun, Shu Zhuo, Huijie Yang, Xiao Yang, Yun Liu, Cheng Yan, Zhongbo Li, Qi Cao, Guimei Ji, Yinlu Ding, Ting Zhuang, Jian Zhu
Summary: The tumor suppressor P53 plays a critical role in preventing breast cancer. RNF187, a ubiquitin ligase, has been found to regulate the P53 signaling pathway and promote breast cancer growth while inhibiting cell apoptosis. The expression of RNF187 is correlated with breast cancer survival only in the P53 wild-type groups. Further experiments reveal that RNF187 interacts with P53 and facilitates its degradation by promoting polyubiquitination, independent of MDM2.
CELL DEATH & DISEASE
(2022)
Article
Genetics & Heredity
Luisa Averdunk, Maxim A. Huetzen, Daniel Moreno-Andres, Reinhard Kalb, Shane McKee, Tzung-Chien Hsieh, Annette Seibt, Marten Schouwink, Seema Lalani, Eissa Ali Faqeih, Theresa Brunet, Peter Boor, Kornelia Neveling, Alexander Hoischen, Barbara Hildebrandt, Elisabeth Graf, Linchao Lu, Weidong Jin, Joerg Schaper, Jamal A. Omer, Tanguy Demaret, Nicole Fleischer, Detlev Schindler, Peter Krawitz, Ertan Mayatepek, Dagmar Wieczorek, Lisa L. Wang, Wolfram Antonin, Ron D. Jachimowicz, Verena von Felbert, Felix Distelmaier
Summary: This study identifies CRIPT gene mutations as being associated with features similar to Rothmund-Thomson syndrome (RTS). The individuals with CRIPT mutations not only exhibit symptoms of RTS, but also have neurodevelopmental delay and seizures. At a cellular level, both CRIPT and RTS mutations lead to increased cellular senescence, suggesting shared molecular mechanisms underlying the clinical phenotypes of these two diseases.
GENETICS IN MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Ting Li, Bo Yan, Xiujuan Xiao, Li Zhou, Jin Zhang, Qiang Yuan, Letian Shan, Huiling Wu, Thomas Efferth
Summary: As a major component of tea, theabrownin (TB) has shown promising anti-cancer potential. This study evaluated the anti-melanoma efficacy of TB using both in vitro and in vivo experiments. TB was found to inhibit the proliferation of melanoma cells and induce DNA damage and apoptosis. It also significantly inhibited the growth of melanoma tumors in a zebrafish xenograft model. The molecular mechanisms of TB involved the activation of DNA damage-associated pathways mediated by p53 and NF-kappa B signaling crosstalk.
Article
Biochemistry & Molecular Biology
Linnea Odborn Jonsson, Maryam Sahi, Ximena Lopez-Lorenzo, Faye Leilah Keller, Ourania N. Kostopoulou, Nikolas Herold, Lars Ahrlund-Richter, Shahrzad Shirazi Fard
Summary: In this study, a comprehensive analysis of cell cycle checkpoint activation following doxorubicin treatment was conducted in TP53 mutated ultra high-risk neuroblastoma cell lines, revealing distinct cell cycle checkpoints and potential druggable targets. The results showed dose-dependent accumulation in S- and/or G2/M-phase, as well as heterogeneous increase of cell cycle checkpoint proteins among the cell lines. Combination treatment with doxo and a small-molecule ATM inhibitor delayed regrowth in specific cell lines, indicating checkpoint dependent and independent targets.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Tzu-Jung Yu, Jen-Yang Tang, Jun-Ping Shiau, Ming-Feng Hou, Chia-Hung Yen, Fu Ou-Yang, Chung-Yi Chen, Hsueh-Wei Chang
Summary: Gin A, a compound isolated from ginger, has been found to inhibit proliferation and induce senescence of breast cancer cells through oxidative stress. It decreases cellular ATP content and cell viability, and delays the G2/M response in breast cancer cells. Additionally, Gin A induces oxidative stress and senescence responses by increasing reactive oxygen species, mitochondrial superoxide, and beta-galactosidase activity, and upregulates senescence-associated gene expressions. Gin A also induces DNA damage responses. These findings suggest the potential anticancer effects of Gin A on breast cancer cells.
Article
Cell Biology
Tadahito Yasuda, Mayu Koiwa, Atsuko Yonemura, Keisuke Miyake, Ryusho Kariya, Sho Kubota, Takako Yokomizo-Nakano, Noriko Yasuda-Yoshihara, Tomoyuki Uchihara, Rumi Itoyama, Luke Bu, Lingfeng Fu, Kota Arima, Daisuke Izumi, Shiro Iwagami, Kojiro Eto, Masaaki Iwatsuki, Yoshifumi Baba, Naoya Yoshida, Hiroto Ohguchi, Seiji Okada, Keisuke Matsusaki, Goro Sashida, Akiko Takahashi, Patrick Tan, Hideo Baba, Takatsugu Ishimoto
Summary: In the tumor microenvironment, senescent cancer-associated fibroblasts (CAFs) play a role in promoting gastric cancer (GC) peritoneal tumor formation through maintenance of the senescence-associated secretory phenotype (SASP) and JAK/STAT3 signaling. Single-cell mass cytometry analysis revealed a high expression level of senescent fibroblasts and SASP factors in the ascites of GC patients. These findings provide insights into the molecular mechanisms of inflammation-related SASP maintenance and the involvement of senescent CAFs in GC peritoneal dissemination.
Article
Plant Sciences
Lei Yu, Shuang Han, Lang Lang, Hui Song, CaiYun Zhang, Lin Dong, ShaoHua Jia, Ying Zhang, Di Xiao, Jun Liu, Ying Xu, XiaoPo Zhang
Summary: Oxocrebanine, an active aporphine alkaloid in Stephania hainanensis, exhibited strong anti-breast cancer activity by inhibiting Topo I and II alpha, leading to DNA damage and mitotic arrest in MCF-7 cells. It acted as a catalytic inhibitor and DNA intercalator, also inducing autophagy and disrupting tubulin polymerization. Oxocrebanine showed potential as a dual Topo inhibitor for effective breast cancer treatment.
Review
Cell Biology
Ruchi Kumari, Parmjit Jat
Summary: Cellular senescence is a stable cell cycle arrest triggered in response to various stimuli, characterized by dynamic changes in senescent cells. It has both positive effects on tissue repair and anti-cancer mechanisms, but can also negatively impact organismal health. Multiple pathways, including p53/p21 and p16/pRB, play central roles in regulating this process.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Oncology
Jiajing Niu, Jiamei Wang, Qi Zhang, Zhihua Zou, Yushuang Ding
Summary: The study revealed that sublethal doses of cinobufagin suppressed the viability of cancer cells by inducing oxidative stress, leading to DNA damage and cell cycle regulation that ultimately resulted in the induction of apoptosis.
CANCER CELL INTERNATIONAL
(2021)
Article
Oncology
Guosheng Lyu, Peng Sue, Xiaohe Hao, Shiming Chen, Shuai Ren, Zixiao Zhao, Yaoqin Gong, Qiao Liu, Changshun Shao
Summary: RECQL4 is highly expressed in ESCC and is associated with poor differentiation, enhanced invasion, and metastasis. It promotes cell proliferation and migration, regulates DNA damage, redox homeostasis, and cell survival.
CANCER BIOLOGY & MEDICINE
(2021)
Article
Cell Biology
Letizia Granieri, Federica Marocchi, Marine Melixetian, Neda Mohammadi, Paola Nicoli, Alessandro Cuomo, Tiziana Bonaldi, Stefano Confalonieri, Federica Pisati, Giuseppina Giardina, Giovanni Bertalot, Daniela Bossi, Luisa Lanfrancone
Summary: This study identifies USP7 as a regulator of melanoma and shows that its downregulation induces cellular senescence, inhibiting tumor growth and proliferation. Transcriptomic and proteomic analysis reveals the targets and networks affected by USP7 depletion. RRM2 is identified as a target of USP7 and is regulated by USP7 during the S phase of the cell cycle. Combination therapy using a USP7 inhibitor and a histone deacetylase (HDAC)/LSD1 inhibitor eliminates senescent cells.
Review
Oncology
Liqin Wang, Lina Lankhorst, Rene Bernards
Summary: This review discusses how senescence can be induced in cancer cells and how the distinctive features of senescent cancer cells can be exploited for selective eradication. It also explores activation of the host immune system as an attractive way to clear senescent cancer cells, and the potential benefits of a sequential treatment approach with pro-senescence therapy followed by senolytic therapy.
NATURE REVIEWS CANCER
(2022)
Article
Biochemistry & Molecular Biology
Riva Shmulevich, Valery Krizhanovsky
Summary: Senescence is a crucial biological process that can block tumorigenesis and limit tissue damage, but accumulation of senescent cells can promote age-related diseases. Recent advances have shown the significant role of cellular metabolism in regulating cell senescence, and future research should focus on elucidating metabolic mechanisms to develop new therapeutic strategies.
ANTIOXIDANTS & REDOX SIGNALING
(2021)
Review
Cell Biology
Hiroshi Ebata, Tze Mun Loo, Akiko Takahashi
Summary: This review summarizes the unique characteristics of cancer cells, such as high proliferation and aberrant DNA damage response, and the role of telomerase and ALT in cancer regulation. It focuses on their noncanonical functions beyond telomere maintenance, as well as intercellular communication in the tumor microenvironment (TME) through the cGAS-STING pathway. Understanding the functional connection between telomere-related molecules and TME regulation is crucial for developing cancer therapeutics targeting specific pathways.
Article
Reproductive Biology
Anna Z. Urbisz, Lukasz Chajec, Karol Malota, Sebastian Student, Marta K. Sawadro, Malgorzata A. Sliwinska, Piotr Swiatek
Summary: This study analyzed the distribution, morphology, and membrane potential of mitochondria in female cysts in Enchytraeus albidus using various microscopy techniques. The findings showed that mitochondria form extensive aggregates and networks in the cysts, which are classified as a dynamic hyperfusion state. This morphology allows for high activity and intensified cellular respiration. Additionally, damaged mitochondria are removed from the networks for elimination. The high activity and dynamic state of the mitochondria are attributed to serving the needs of growing oocytes in the cysts.
BIOLOGY OF REPRODUCTION
(2022)
Article
Nutrition & Dietetics
Caroline Sarah Stokes, Daniela Weber, Stefan Wagenpfeil, Wolfgang Stuetz, Maria Moreno-Villanueva, Martijn E. T. Dolle, Eugene Jansen, Efstathios S. Gonos, Juergen Bernhardt, Beatrix Grubeck-Loebenstein, Simone Fiegl, Ewa Sikora, Olivier Toussaint, Florence Debacq-Chainiaux, Miriam Capri, Antti Hervonen, P. Eline Slagboom, Nicolle Breusing, Jan Frank, Alexander Buerkle, Claudio Franceschi, Tilman Grune
Summary: Lower levels of vitamin A and D are independently associated with fair/poor self-rated health, while no significant associations were observed for alpha-tocopherol levels. Further research is needed to explore the long-term implications of these modifiable nutrients on health status.
BRITISH JOURNAL OF NUTRITION
(2022)
Article
Oncology
Jinsong Liu, Jekaterina Erenpreisa, Ewa Sikora
SEMINARS IN CANCER BIOLOGY
(2022)
Article
Cell Biology
Agnieszka Grabowska, Hanna Sas-Nowosielska, Bartosz Wojtas, Dagmara Holm-Kaczmarek, Elzbieta Januszewicz, Yana Yushkevich, Iwona Czaban, Pawel Trzaskoma, Katarzyna Krawczyk, Bartlomiej Gielniewski, Ana Martin-Gonzalez, Robert Kuba Filipkowski, Krzysztof Hubert Olszynski, Tytus Bernas, Andrzej Antoni Szczepankiewicz, Malgorzata Alicja Sliwinska, Tambudzai Kanhema, Clive R. Bramham, Grzegorz Bokota, Dariusz Plewczynski, Grzegorz Marek Wilczynski, Adriana Magalska
Summary: Spatial chromatin organization is crucial for transcriptional regulation in neurons and is influenced by activity-induced chromatin condensation, histone modifications, and rearrangements in chromosome territories and nuclear speckles. HDAC1 plays a key role in this process.
Article
Psychology, Multidisciplinary
Jennifer Nicola M. Rea, Katarzyna Milana Broczek, Elisa Cevenini, Laura Celani, Susanne Alexandra J. Rea, Ewa Sikora, Claudio Franceschi, Vita Fortunati, Irene Maeve Rea
Summary: Understanding how to age longer and age well is a priority for individuals, populations, and government policies. Nonagenarians have rich life experiences and genetic, lifestyle, and psychological information that can help us understand how to live longer and better. Research in four European countries found that nonagenarians demonstrate high positivity, resilience, and coping skills, and are supported by social networks.
FRONTIERS IN PSYCHOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Joanna Czarnecka-Herok, Malgorzata Alicja Sliwinska, Marcin Herok, Alicja Targonska, Anna Strzeszewska-Potyrala, Agnieszka Bojko, Artur Wolny, Grazyna Mosieniak, Ewa Sikora
Summary: This study investigated the cellular senescence and polyploidization of cancer cells after anticancer treatment, and found that the cells undergo temporary arrest of cell division followed by polyploidization and altered gene expression related to meiotic cell cycles, spermatogenesis, and epithelial-mesenchymal transition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Geriatrics & Gerontology
Robertina Giacconi, Patrizia D'Aquila, Marco Malavolta, Francesco Piacenza, Alexander Burkle, Maria Moreno Villanueva, Martijn E. T. Dolle, Eugene Jansen, Tilman Grune, Efstathios S. Gonos, Claudio Franceschi, Miriam Capri, Daniela Gradinaru, Beatrix Grubeck-Loebenstein, Ewa Sikora, Wolfgang Stuetz, Daniela Weber, Olivier Toussaint, Florence Debacq-Chainiaux, Antti Hervonen, Mikko Hurme, P. Eline Slagboom, Christiane Schon, Jurgen Bernhardt, Nicolle Breusing, Talbot Duncan, Giuseppe Passarino, Dina Bellizzi, Mauro Provinciali
Summary: This study found that the concentration of blood bacterial DNA (BB-DNA) was higher in the general population compared to individuals from long-living families and their spouses. BB-DNA was found to be associated with smoking, Charlson Comorbidity Index (CCI), leukocyte subsets, and some oxidative stress markers in the general population.
JOURNALS OF GERONTOLOGY SERIES A-BIOLOGICAL SCIENCES AND MEDICAL SCIENCES
(2023)
Article
Geriatrics & Gerontology
Agata Gluchowska, Dominik Cysewski, Monika Baj-Krzyworzeka, Rafal Szatanek, Kazimierz Weglarczyk, Paulina Podszywalow-Bartnicka, Piotr Sunderland, Ewa Kozlowska, Malgorzata A. Sliwinska, Michal Dabrowski, Ewa Sikora, Grazyna Mosieniak
Summary: Atherosclerosis is a common age-related disease characterized by intense immunological activity. Senescent vascular smooth muscle cells (VSMCs) secrete increased number of extracellular vesicles (senEVs) that modulate the cytokine milieu by influencing immune cell activity.
Article
Geriatrics & Gerontology
Laura Kananen, Mikko Hurme, Alexander Buerkle, Maria Moreno-Villanueva, Jurgen Bernhardt, Florence Debacq-Chainiaux, Beatrix Grubeck-Loebenstein, Marco Malavolta, Andrea Basso, Francesco Piacenza, Sebastiano Collino, Efstathios S. Gonos, Ewa Sikora, Daniela Gradinaru, Eugene H. J. M. Jansen, Martijn E. T. Dolle, Michel Salmon, Wolfgang Stuetz, Daniela Weber, Tilman Grune, Nicolle Breusing, Andreas Simm, Miriam Capri, Claudio Franceschi, Eline Slagboom, Duncan Talbot, Claude Libert, Jani Raitanen, Seppo Koskinen, Tommi Harkanen, Sari Stenholm, Mika Ala-Korpela, Terho Lehtimaki, Olli T. Raitakari, Olavi Ukkola, Mika Kahonen, Marja Jylha, Juulia Jylhava
Summary: Circulating cell-free DNA (cf-DNA) levels are associated with age, sex, smoking, physical activity, vegetable consumption, ageing phenotypes, and various biomarkers. Higher cf-DNA levels were found in men, especially middle-aged men and women who smoke, and older frail individuals. Inflammatory markers, metabolites, and antioxidant levels were correlated with cf-DNA levels. These findings have implications for future studies on the epidemiology and biological mechanisms of cf-DNA and its potential as a clinical marker.
Article
Cell Biology
Adam Krzystyniak, Malgorzata Wesierska, Gregory Petrazzo, Agnieszka Gadecka, Magdalena Dudkowska, Anna Bielak-Zmijewska, Grazyna Mosieniak, Izabela Figiel, Jakub Wlodarczyk, Ewa Sikora
Summary: By conducting experiments on aged rats, it was found that the use of senolytic agents D+Q can improve the learning and memory abilities of aged rats, reduce peripheral inflammation, and regulate the structure of hippocampal neurons and histone modifications. Moreover, the benefits of this treatment are long-lasting, even after the cessation of drug administration.
Article
Biochemistry & Molecular Biology
Kun He, Mengyi Xie, Weifeng Hong, Yonghe Li, Yaolin Yin, Xiaojin Gao, Yi He, Yu Chen, Chuan You, Jingdong Li
Summary: Centromere protein L (CENPL) is overexpressed in hepatocellular carcinoma (HCC) and is associated with poor patient prognosis. Upregulation of CENPL promotes tumor proliferation and glycolysis in HCC cells by activating the MEK1/2-ERK1/2 signaling pathway.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Yingzi Wang, Haozhong Huang, Huimin Weng, Chunsen Jia, Bin Liao, Yang Long, Fengxu Yu, Yongmei Nie
Summary: Talin protein plays a crucial role in transmitting mechanical forces by connecting the extracellular matrix to the cytoskeleton. It converts mechanical signals into biochemical signals and serves as diagnostic, therapeutic, and prognostic indicators in diseases.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Review
Biochemistry & Molecular Biology
Hema Saranya Ilamathi, Marc Germain
Summary: Mitochondria are the central metabolic hubs in cells, relying on proteins encoded by nuclear DNA and mitochondrial DNA (mtDNA) for their function. The maintenance and distribution of mtDNA are crucial for proper mitochondrial function and are associated with mitochondrial diseases. mtDNA is organized into nucleoids that dynamically move and interact with each other. The replication and distribution of mtDNA nucleoids are regulated by the complex interplay of mitochondrial dynamics, ER-mitochondria contact sites, and cytoskeletal networks.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
K. K. Sruthi, Sirisha Natani, Ramesh Ummanni
Summary: The overexpression of TPD52 is associated with the emergence of neuroendocrine prostate cancer (NEPC). TPD52 activates the NF-kappa B - STAT3 axis to induce neuroendocrine differentiation (NED) of prostate cancer cells. Therapeutic targeting of TPD52 is important for the treatment of prostate cancer.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)
Article
Biochemistry & Molecular Biology
Cui Li, Feifan Xiang, Yuqi Gong, Yi Fu, Ge Chen, Zhi Wang, Zhong Li, Daiqing Wei
Summary: Our study demonstrates the significant role of tumor-derived microparticles (T-MPs) in osteosarcoma metastasis and immune response. T-MPs promote macrophage polarization and stimulate migration and chemoresistance of osteosarcoma cells, thereby affecting the progression of osteosarcoma.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2024)