Automation and Expansion of EMMA Assembly for Fast-Tracking Mammalian System Engineering

EMMA-CAD: Design Automation for Synthetic Mammalian Constructs

(2022) Yisha Luo et al.   ACS Synthetic Biology

Genetic Toolkits to Design and Build Mammalian Synthetic Systems

(2021) Roberto Di Blasi et al.   TRENDS IN BIOTECHNOLOGY

Cell-Free Protein Synthesis as a Prototyping Platform for Mammalian Synthetic Biology

(2020) Margarita B. Kopniczky et al.   ACS Synthetic Biology

CRISPR-Based Synthetic Transcription Factors In Vivo: The Future of Therapeutic Cellular Programming

(2020) Matthew Pandelakis et al.   Cell Systems

Multiplexed CRISPR technologies for gene editing and transcriptional regulation

(2020) Nicholas S. McCarty et al.   Nature Communications

Automated Continuous Evolution of Proteins in Vivo

(2020) Ziwei Zhong et al.   ACS Synthetic Biology

Enabling one-pot Golden Gate assemblies of unprecedented complexity using data-optimized assembly design

(2020) John M. Pryor et al.   PLoS One

Standardizing Automated DNA Assembly: Best Practices, Metrics, and Protocols Using Robots

(2019) David I. Walsh et al.   SLAS Technology

Modular one-pot assembly of CRISPR arrays enables library generation and reveals factors influencing crRNA biogenesis

(2019) Chunyu Liao et al.   Nature Communications

Systematic Evaluation of CRISPRa and CRISPRi Modalities Enables Development of a Multiplexed, Orthogonal Gene Activation and Repression System

(2019) Andrea Martella et al.   ACS Synthetic Biology

Search-and-replace genome editing without double-strand breaks or donor DNA

(2019) Andrew V. Anzalone et al.   NATURE

A Toolkit for Rapid Modular Construction of Biological Circuits in Mammalian Cells

(2019) João Pedro Fonseca et al.   ACS Synthetic Biology

Point-of-care production of therapeutic proteins of good-manufacturing-practice quality

(2018) Rajani Adiga et al.   Nature Biomedical Engineering

Comprehensive Profiling of Four Base Overhang Ligation Fidelity by T4 DNA Ligase and Application to DNA Assembly

(2018) Vladimir Potapov et al.   ACS Synthetic Biology

EMMA: An Extensible Mammalian Modular Assembly Toolkit for the Rapid Design and Production of Diverse Expression Vectors

(2017) Andrea Martella et al.   ACS Synthetic Biology

Automated Robotic Liquid Handling Assembly of Modular DNA Devices

(2017) Luis Ortiz et al.   Jove-Journal of Visualized Experiments

CRISPR/Cas9 in Genome Editing and Beyond

(2016) Haifeng Wang et al.   Annual Review of Biochemistry

Registry in a tube: multiplexed pools of retrievable parts for genetic design space exploration

(2016) Lauren B. A. Woodruff et al.   NUCLEIC ACIDS RESEARCH

A Modular Assembly Platform for Rapid Generation of DNA Constructs

(2016) Elliot H. Akama-Garren et al.   Scientific Reports

Engineering Complex Synthetic Transcriptional Programs with CRISPR RNA Scaffolds

(2015) Jesse G. Zalatan et al.   CELL

Bricks and blueprints: methods and standards for DNA assembly

(2015) Arturo Casini et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Principles of genetic circuit design

(2014) Jennifer A N Brophy et al.   NATURE METHODS

A platform for rapid prototyping of synthetic gene networks in mammalian cells

(2014) Xavier Duportet et al.   NUCLEIC ACIDS RESEARCH

Multiplex CRISPR/Cas9-based genome engineering from a single lentiviral vector

(2014) Ami M. Kabadi et al.   NUCLEIC ACIDS RESEARCH

Multiplex genome engineering in human cells using all-in-one CRISPR/Cas9 vector system

(2014) Tetsushi Sakuma et al.   Scientific Reports

Rapid, modular and reliable construction of complex mammalian gene circuits

(2013) Patrick Guye et al.   NUCLEIC ACIDS RESEARCH

RNA-Guided Human Genome Engineering via Cas9

(2013) P. Mali et al.   SCIENCE

Multiplex Genome Engineering Using CRISPR/Cas Systems

(2013) L. Cong et al.   SCIENCE

Systematic dissection and optimization of inducible enhancers in human cells using a massively parallel reporter assay

(2012) Alexandre Melnikov et al.   NATURE BIOTECHNOLOGY

A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity

(2012) M. Jinek et al.   SCIENCE

PaR-PaR Laboratory Automation Platform

(2012) Gregory Linshiz et al.   ACS Synthetic Biology

Programming cells by multiplex genome engineering and accelerated evolution

(2009) Harris H. Wang et al.   NATURE

A Robotic Platform for Quantitative High-Throughput Screening

(2008) Sam Michael et al.   ASSAY AND DRUG DEVELOPMENT TECHNOLOGIES

A One Pot, One Step, Precision Cloning Method with High Throughput Capability

(2008) Carola Engler et al.   PLoS One