Journal
INTERNATIONAL JOURNAL FOR PARASITOLOGY
Volume 46, Issue 1, Pages 7-12Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ijpara.2015.09.007
Keywords
Virtual Northern; RNA-seq; Plasmodium falciparum; Malaria; Untranslated region; Transcript
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Funding
- Thailand Research Fund [RSA5780007]
- Platform-BIOTEC (Thailand) [P-12-01270]
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Accurate gene models are essential for understanding parasite biology. However, transcript structure information is lacking for most parasite genes. Here, we describe Virtual Northern analysis of the malaria parasite Plasmodium falciparum to address this issue. RNA-seq libraries were made from size fractionated RNA. Transcript sizes for 3052 genes were inferred from the read counts in each library. The data show that for almost half of the transcripts, the combined untranslated regions are more than twice the length of the open reading frame. Furthermore, we identified novel polycistronic, or gene overlapping, transcripts that suggest revisions to current gene models are needed. (C) 2015 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.
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