Journal
VIRUSES-BASEL
Volume 13, Issue 10, Pages -Publisher
MDPI
DOI: 10.3390/v13102020
Keywords
Crocidura paramyxovirus; novel virus discovery; next-generation sequencing; genetic characterization and diversity; potential zoonosis
Categories
Funding
- Agency for Defense Development [UE202026GD]
- National Research Foundation of Korea (NRF) - Ministry of Education [NRF-2021R1I1A2049607]
- Korea Institute of Marine Science & Technology Promotion in the Ministry of Oceans and Fisheries (MOF) of South Korea [21210466]
- Armed Forces Health Surveillance Division Global Emerging Infections Surveillance Branch (GEIS) [P0039_18_ME]
Ask authors/readers for more resources
Paramyxoviruses, a critical threat to human public health, were found in shrews in Korea, with two genetically distinct novel Henipaviruses identified. The study sheds light on the genetic characteristics, diversity, and zoonotic potential of these viruses in shrews.
Paramyxoviruses, negative-sense single-stranded RNA viruses, pose a critical threat to human public health. Currently, 78 species, 17 genera, and 4 subfamilies of paramyxoviruses are harbored by multiple natural reservoirs, including rodents, bats, birds, reptiles, and fish. Henipaviruses are critical zoonotic pathogens that cause severe acute respiratory distress and neurological diseases in humans. Using reverse transcription-polymerase chain reaction, 115 Crocidura species individuals were examined for the prevalence of paramyxovirus infections. Paramyxovirus RNA was observed in 26 (22.6%) shrews collected at five trapping sites, Republic of Korea. Herein, we report two genetically distinct novel paramyxoviruses (genus: Henipavirus): Gamak virus (GAKV) and Daeryong virus (DARV) isolated from C. lasiura and C. shantungensis, respectively. Two GAKVs and one DARV were nearly completely sequenced using next-generation sequencing. GAKV and DARV contain six genes (3 & PRIME;-N-P-M-F-G-L-5 & PRIME;) with genome sizes of 18,460 nucleotides and 19,471 nucleotides, respectively. The phylogenetic inference demonstrated that GAKV and DARV form independent genetic lineages of Henipavirus in Crocidura species. GAKV-infected human lung epithelial cells elicited the induction of type I/III interferons, interferon-stimulated genes, and proinflammatory cytokines. In conclusion, this study contributes further understandings of the molecular prevalence, genetic characteristics and diversity, and zoonotic potential of novel paramyxoviruses in shrews.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available