Journal
THERIOGENOLOGY
Volume 179, Issue -, Pages 155-161Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.theriogenology.2021.11.023
Keywords
Sheep; Cry2; Insertion; deletion (InDel); Litter size; Marker-assisted selection (MAS)
Categories
Funding
- Col-lege of Animal Science and Technology of Northwest AF Univer-sity [JS20210411001, JS20200507001]
- Tianjin Municipal Key Research and Development Program [20YFZCSN00720]
- Tianjin Municipal Science and Technology Plan Project [21ZYCGSN00540]
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This study investigates the polymorphisms of the Cry2 gene in Australian White ewes and finds two novel deletion mutations that are significantly associated with litter size. These findings may contribute to molecular breeding strategies in sheep.
Cryptochrome 2 (Cry2) gene regulates circadian rhythm and affects reproduction and pregnancy. Therefore, this study aimed to explore polymorphisms of the Cry2 gene and their associations with litter size at different parity in Australian White (AuW) ewes. Five putative insertion or deletion mutations within the Cry2 gene were selected to study their association with litter size. Two novel deletion mutations were identified in intronic region of Cry2 gene and were genotyped by agarose gel electrophoresis and DNA sequencing. The polymorphism information content (PIC) indicated that both mutations were low polymorphism in tested groups. Statistical analysis revealed that the P1-Del-6-bp was significantly correlated with litter size at third parity (P = 0.010), in which individuals with insertion/deletion (ID) genotype had larger litter size than insertion/insertion (II) genotype (P < 0.05). Whereas, the P2-Del-6-bp was significantly correlated with litter size at first parity (P = 0.036), in which individuals with insertion/ insertion (II) genotype had larger litter size than insertion/deletion (ID) genotype (P < 0.05). Collectively, these findings may provide new insights to expedite molecular breeding in sheep through marker assisted selection strategies (MAS).(c) 2021 Elsevier Inc. All rights reserved.
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