Article
Spectroscopy
Malgorzata Swierczynska, Daniel Slowinski, Radoslaw Michalski, Jaroslaw Romanski, Radoslaw Podsiadly
Summary: A simple thiomorpholine-based fluorescent probe was designed and synthesized for detecting hypochlorous acid (HOCl). The probe effectively quenches the fluorescence of the fluorophore through photoinduced electron transfer (PET) effect. It has high sensitivity and selectivity, reliable performance at physiological pH, and rapid fluorescence response, making it suitable for detecting HOCl produced by myeloperoxidase. The probe shows promise as a screening tool for MPO inhibitors due to its high reaction rate constant with HOCl.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Multidisciplinary
Wei Hu, Taotao Qiang, Chenchen Li, Longfang Ren, Fei Cheng, Baoshuai Wang, Mingli Li, Xinjian Song, Tony D. James
Summary: Small-molecule NIR imaging is a popular technique for tracking biological species and events due to its advantages such as deep tissue penetration, low autofluorescence, non-invasive visualization, and simple operation. However, the small Stokes shift of most NIR dyes often leads to low signal-to-noise ratio and self-quenching. In this research, a NIR-based fluorescent probe was developed for hypochlorous acid detection, which successfully addressed the issue of crosstalk between excitation and emission spectra. Imaging studies demonstrated the probe's potential for endogenous HOCl detection and imaging in living cells.
Article
Spectroscopy
Huimin Shangguan, Qianwei Liu, Yuanjuan Wang, Zixuan Teng, Rumeng Tian, Tong Wu, Lei Yang, Ling Jiang, Xingjiang Liu, Liuhe Wei
Summary: In this study, a chromenoquinoline-based ratiometric fluorescent probe was developed for the detection and quantification of hypochlorous acid (HClO). The probe exhibited a large Stokes shift, high synthetic yield, simple synthesis method, short response time, low detection limit, and low toxicity. The probe was successfully validated in live cells and zebrafish.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Applied
Lijuan Liang, Yimeng Sun, Chang Liu, Xianshun Zeng, Jianlong Zhao
Summary: A novel probe PTZ-2Cy has been developed for selective detection of ClO- through oxidation and cleavage of C??C bonds, showing low detection limit and excellent selectivity. The probe is capable of imaging HClO in L929 cells and holds promise for detecting hypochlorite ions in biological samples.
Article
Chemistry, Analytical
Songjiao Li, Peipei Wang, Ke Yang, Ying Liu, Dan Cheng, Longwei He
Summary: In order to improve the prognosis and overall survival of hepatocellular carcinoma (HCC) patients, it is important to identify and resect it at an early stage. However, developing specific fluorescent probes to detect hypochlorous acid (HClO) in liver cells is still challenging. Therefore, the development of a new probe MBH-MT that can detect HClO in biological samples and image it in cells and tissues is of great significance for the diagnosis and treatment of HCC.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Bingya Wang, Fengying Yuan, Shan Wang, Ran Duan, Wen Xiu Ren, Ji-Ting Hou
Summary: Atherosclerosis is closely related to cardiovascular and cerebrovascular diseases, being major causes of global mortality. Early diagnosis of atherosclerosis can aid in the prevention and treatment of associated diseases. A new fluorescent probe, AS-ClO, was developed for detecting mitochondrial HOCl in foam cells, showing high selectivity and sensitivity.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Chang Liu, Xiaojie Jiao, Songtao Cai, Song He, Liancheng Zhao, Xianshun Zeng
Summary: Endogenous reactive oxygen species and reductive sulphur species play crucial roles in biological functions, influencing calcium homeostasis in neurons. A novel fluorescent probe PzSe with a reversible selenium atom can detect the redox cycle of HOCl/H2S with high sensitivity and selectivity. Through three-color fluorescence changes in PC-12 cells co-stained with PzSe and Ca2+-selective Fluo-4 AM, dynamic correlations among HOCl, H2S, and Ca2+ in neurons can be studied.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Applied
Guangshuai Zhou, Shaobo Hou, Ning Zhao, Nathaniel Finney, Yali Wang
Summary: A highly sensitive ratiometric fluorescent probe, NAP-RS, was designed for measuring fluctuations of lysosomal HOCl. The probe exhibited a clear fluorescence response to HOCl at biologically-relevant concentrations, with rapid response time and ultrasensitivity. Additionally, it could be used for visualizing exogenous and endogenous lysosomal HOCl.
Article
Chemistry, Analytical
Fengying Yuan, Bingya Wang, Ji-Ting Hou, Jiali Li, Jianliang Shen, Yi Duan, Wen Xiu Ren, Shan Wang
Summary: In this study, we proposed a HOCl-specific fluorescent probe for monitoring liver fibrosis (LF) progression. The probe has the advantages of easy preparation, high selectivity, superb sensitivity, rapid response, and good membrane permeability, and can monitor delicate changes in cellular HOCl levels and discriminate different cell types. Experimental results indicate the potential of HOCl as a unique biomarker for LF detection and clinical diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Xiao-Peng Fan, Jing Huang, Tian-Bing Ren, Lin Yuan, Xiao-Bing Zhang
Summary: This study developed a novel near-infrared hemicyanines fluorophore (FJH) with good hydrophilicity and blood circulation, enabling lung imaging. The probe FJH-C showed selectivity and sensitivity towards the ALI indicator (esterase) in vitro and in living cells. This research provides a new method for real-time lung functional imaging and highlights the fluorophore structure with high lung retention.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Aishan Zheng, Han Liu, Chuanhao Peng, Xiaonan Gao, Kehua Xu, Bo Tang
Summary: CVS is a mitochondria-targeting near-Infrared fluorescent probe designed for sensing and detecting HOCl with high selectivity and low detection limit. It has been successfully applied to image HOCl in mitochondria of HepG2 cells, providing a perspective method for further study of physiological and pathological functions related to HOCl.
Article
Materials Science, Biomaterials
Weiyao Zhang, Wenhui Song, Weiying Lin
Summary: Diabetes is closely related to excess HClO induced by endoplasmic reticulum stress, leading to the design of a novel fluorescent probe for detection. High glucose levels have been found to induce oxidative stress in the endoplasmic reticulum and produce excessive HClO, with the probe successfully used for tissue imaging in diabetic mice.
JOURNAL OF MATERIALS CHEMISTRY B
(2021)
Article
Chemistry, Analytical
Yuanqiang Hao, Yintang Zhang, Qiuling Sun, Shu Chen, Zilong Tang, Rongjin Zeng, Maotian Xu
Summary: Hypochlorous acid (HOCl) is a potent reactive oxygen species essential in biological processes. A new ratiometric fluorescent probe has been designed and synthesized for sensitive and selective detection of HOCl, with advantages including rapid response, high signal to noise ratio, and utility for visualizing HOCl in cells and organisms.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
Songjiao Li, Ke Yang, Peipei Wang, Ying Liu, Dan Cheng, Longwei He
Summary: Heat stroke is a devastating pathology with high morbidity and mortality rates. Understanding the cytotoxic mechanisms of heat can help prevent or treat stroke-related diseases. The role of lysosomes in heat stroke is poorly understood due to a lack of tools, but recent studies have shown a correlation between lysosome HClO and heat stroke development. To better interpret and treat heat stroke, a reliable real-time tool for tracking HClO levels is necessary, and MB-HClO, a near-infrared probe, shows promise in rapidly detecting lysosome HClO.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Applied
Yadan Huo, Yuan Ji, Dingguo Chen, Shouzhi Pu
Summary: The study presents the design of a mitochondrial-targeted probe HDCl for fast and sensitive detection of HClO in living cells, which can be successfully applied for mitochondrial imaging.
Article
Chemistry, Analytical
Mengmeng Guo, Na Luo, Yueling Bai, Zhenggang Xue, Qingmin Hu, Jiaqiang Xu
Summary: A porous heterostructure WO3-C/In2O3 was designed and prepared for a miniature H2 sensor, which showed higher response value, lower operating temperature, fast response-recovery speed, and low limit of detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Feng Hu, Hui Hu, Yuting Li, Xiaohui Wang, Xiaowen Shi
Summary: Arsenic contamination in water bodies is a significant health risk. This study developed a chitosan-catechol modified electrode for rapid and accurate detection of trace amounts of arsenic. The modified electrode demonstrated good detection capability and resistance to ionic interference, making it suitable for in situ detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Yantao Zhang, Qian Liu, Tao Tian, Chunhua Xu, Pengli Yang, Lianju Ma, Yi Hou, Hui Zhou, Yongjun Gan
Summary: In this study, a lysosome-targeting buffering fluorogenic probe (Lyso-BFP) was designed and synthesized, demonstrating excellent photostability, pH specificity, and responsiveness to lysosomal acidification in living cells. The performance of Lyso-BFP in pH sensing was attributed to the inhibition of the photo-induced electron transfer process. Lyso-BFP allowed for wash-free imaging and long-term real-time monitoring of lysosome pH changes based on its off-on fluorescence behavior and buffer strategy.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Wei Cai, Wenbo Sun, Jiayue Wang, Xiaokui Huo, Xudong Cao, Xiangge Tian, Xiaochi Ma, Lei Feng
Summary: In this study, a near-infrared fluorescent probe HCBG was developed for imaging of alpha-GLC. HCBG exhibited excellent selectivity and sensitivity towards alpha-GLC in complex bio-samples, and showed good cell permeability for in situ real-time imaging. Through the high-throughput screening system established by HCBG, a natural alpha-GLC inhibitor was successfully isolated and identified. This study provides a novel fluorescence visualization tool for discovering and exploring the biological functions of diabetes-related gut microbiota, and a high-throughput screening approach for alpha-GLC inhibitor.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Trey W. Pittman, Xi Zhang, Chamindie Punyadeera, Charles S. Henry
Summary: Heart failure is a growing epidemic and a significant clinical and public health problem. Researchers have developed a portable and affordable diagnostic device for heart failure that can be used at the point-of-care, providing a valid alternative to current diagnostics approaches.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Anders O. Tjell, Barbara Jud, Roland Schaller-Ammann, Torsten Mayr
Summary: An optical hydrogen peroxide sensor based on catalytic degradation and the detection of produced oxygen is presented. The sensor offers higher resolution and better sensitivity at lower H2O2 concentrations. By removing O2 from the sample solution, a more sensitive O2 sensor can be used for measurement. The sensor has been successfully applied in a flow-through cell to measure H2O2 concentration in different flow rates.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Seong Jae Kim, Ji-hun Jeong, Gaabhin Ryu, Yoon Sick Eom, Sanha Kim
Summary: Surface-enhanced Raman spectroscopy (SERS) is a high-sensitivity, label-free detection method with various analytical applications. Researchers have developed a hydrophobic SERS substrate based on engineered carbon nanotube arrays (CNT-SERS) and studied the role of structural design at both micro and nanoscales. The substrate demonstrated controlled self-enrichment capability and enhanced sensitivity, with a significant increase in the SERS signal. The study also proposed a theoretical model and a concentration strategy inspired by plants for analyte deposition on microarrays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Dan Zhao, Renjun Jiang, Xiaoqiang Liu, Subbiah Alwarappan
Summary: In this study, a novel ternary composite material was constructed by assembling cerium vanadate nanorods on reduced graphene oxide-microcrystalline cellulose nanosheets, and it was used for real-time monitoring of the concentration of superoxide anions in vivo. The ternary composite showed excellent conductivity, large surface area, and abundant active sites, leading to a wider linear range, high sensitivity, low detection limit, and fast response time for superoxide anion detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Tengfei Wang, Liwen Wang, Guang Wu, Dating Tian
Summary: In this study, a covalent organic framework material TaTp-COF with porous and uniform spheres was successfully prepared via hydrothermal reaction, and it was found to significantly enhance the aggregation-induced emission (AIE) of berberine. The unique emission properties of berberine on TaTp-COF were studied and utilized for the sensitive detection of berberine.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Lin Li, Yilei Ding, Lei Xu, Shuoran Chen, Guoliang Dai, Pengju Han, Lixin Lu, Changqing Ye, Yanlin Song
Summary: In this study, a novel TTI based on a ratiometric fluorescent nanosensor is designed, which has the advantages of high accuracy and low cost. Experimental and theoretical investigations confirm its pH responsiveness and demonstrate its good sensitivity and reliability. By monitoring the total volatile basic nitrogen, this TTI can accurately predict food spoilage and can be adaptively modified for different types of food. The TTI based on this nanosensor enables visual monitoring of food quality.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Fangju Chen, Xueting Wang, Wei Chen, Chenwen Shao, Yong Qian
Summary: Lung cancer is the second most common malignant tumor worldwide. Drug resistance in lung cancer leads to treatment failure and recurrence in majority of patients. This study developed a fluorescent prodrug that can be activated in cancer cells to release drugs, and its signal can be tracked by imaging. It shows a unique autophagy-driven ferroptosis effect, indicating its potential for targeting drug-resistant cancer cells.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Weichao Li, Qiming Yuan, Zhangcheng Xia, Xiaoxue Ma, Lifang He, Ling Jin, Xiangfeng Chu, Kui Zhang
Summary: This study developed a high-performance gas sensor for formaldehyde detection by modifying ZnSnO3 with ZnO QDs and SnO2 QDs. The modified sensor showed improved sensing response and lower working temperature. The presence of ZnO QDs formed rich heterojunctions, increased surface area, and provided oxygen deficiency for formaldehyde sensing reaction, thus enhancing the sensor performance. This research provides an alternative method to enhance the sensing properties of MOS by QDs modification.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Joung-Il Moon, Eun Jung Choi, Younju Joung, Jin-Woo Oh, Sang-Woo Joo, Jaebum Choo
Summary: A novel nanoplasmonic substrate was developed for biomedical applications, which showed strong hot spots for detecting biomarkers at low concentrations. The substrate, called AuNPs@M13, was made by immobilizing 60 nm gold nanoparticles onto the surface of an M13 bacteriophage scaffold. It demonstrated higher sensitivity and lower limit of detection compared to commercially available assays.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Ning Li, Ya Zhang, Ying Xu, Xiaofang Liu, Jian Chen, Mei Yang, Changjun Hou, Danqun Huo
Summary: The molecular subtype of breast cancer guides treatment and drug selection. Invasive tests can promote cancer cell metastasis, so the development of high-performance, low-cost diagnostic tools for cancer prognosis is crucial. Liquid biopsy techniques enable noninvasive, real-time, dynamic, multicomponent, quantitative, and long-term observations at the cellular, genetic, and molecular levels. A Cu-Zr metal-organic framework (MOF) nanoenzyme with monatomic Cu attachment has been synthesized and proven to have high catalytic performance. The sensor constructed using this nanoenzyme shows potential for accurate classification of breast cancer serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Chemistry, Analytical
Jeongmin Kim, Hyemin Kim, Seunghyun Park, Hyeonaug Hong, Yong Jae Kim, Jiyong Lee, Jaeho Kim, Seung-Woo Cho, Wonhyoung Ryu
Summary: This study presents a method to fabricate independently functioning microneedle (MN) electrodes with narrow intervals for high precision electrochemical sensing. The optimized mixture of photocurable polymer and single-wall carbon nanotubes was used to mold single composite MNs, which were then attached to pre-patterned electrodes. Plasma etching and electropolymerization were performed to enhance the electrochemical activity, and Prussian blue and glucose oxidase were electrodeposited on the MNs for glucose detection. The MN electrodes showed good sensitivity and linearity, and the feasibility of glucose detection was demonstrated in an in vivo mouse study.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)