Erythromycin stimulates rather than inhibits methane production in anaerobic digestion of antibiotic fermentation dregs

Erythromycin fermentation dregs (EFD) as one kind of organic-rich biosolid was of great potential for methane production. However, the influence of residual erythromycin (ERY) on the anaerobic digestion process of EFD remains unclear. In this study, a batch test was conducted with different ERY concentrations to investigate its effects on methanogenesis. The antibiotic resistance genes and microbial community composition were analyzed to explore the potential mechanism. The results showed that more than 80% of ERY was removed after 30 days digestion. Furthermore, 100, 200 and 300 mg/L of ERY presented no significant effect on the performance of anaerobic digestion. Instead, a high concentration of ERY (500 mg/L) increased 13% rather than inhibited the methane yields. Moreover, the proliferation of the methylase gene (e.g., ermA/T) was promoted under the high pressure of ERY. The relative abundance of acetogenic bacteria (Sedimentibacter) and mixotrophic archaea (Methanosarcina) were enhanced, indicating that their syntrophic association would play the dominant role in the stimulating effects of methanogenesis. (c) 2021 Elsevier B.V. All rights reserved.

Automated summary

This study found that a high concentration of erythromycin contributes to an increase in methane production, while concentrations of 100, 200, and 300 mg/L had no significant impact on anaerobic digestion performance. Additionally, under high pressure, the proliferation of the methylase gene was promoted, and the relative abundance of acetogenic bacteria and mixotrophic archaea increased.