4.7 Review

Pathogens and disease vectors/hosts monitoring in aquatic environments: Potential of using eDNA/eRNA based approach

Journal

SCIENCE OF THE TOTAL ENVIRONMENT
Volume 796, Issue -, Pages -

Publisher

ELSEVIER
DOI: 10.1016/j.scitotenv.2021.148810

Keywords

Environmental DNA; Environmental RNA; eDNA; eRNA; Infectious diseases; Vector-borne diseases; Monitoring; Aquatic environments

Funding

  1. Japan Society for the Promotion of Science [19K21985, 20K20635]
  2. Kitasato University Graduate School of Medical Sciences
  3. School of Allied Health Sciences Special Re-search Grant [2020-1001]

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This article discusses the spread of infectious diseases in various geographical regions and the impact of environmental factors. It suggests the use of environmental DNA and RNA to monitor pathogens and vectors/hosts, and summarizes their application and advantages in aquatic samples.
Infectious diseases are spreading in to previously unreported geographical regions, and are reappeared in regions 75 or 100 years after their last reported case, as a result of environmental changes caused by anthropogenic activities. A pathogen, vector/host monitoring methodology is therefore indispensable in identifying potential transmission sites, providing early warnings and evaluating the human health risks of these infectious diseases in a given area. Recently, environmental DNA (eDNA) and environmental RNA approach (eRNA) have become widespread in monitoring organisms in the environment due to advantages like lower cost, time, and labour requirements. However, eDNA/eRNA based monitoring of pathogens and vectors/hosts using aquatic samples is limited to very few studies. In this review, we summarized the currently available eDNA/eRNA based human and non-human pathogens and vectors/hosts detection studies in aquatic samples. Species-specific shedding, transport, and decay of eDNA/eRNA in aquatic environments which is essential in estimating the abundance of pathogen, vectors/host in focus is also summarized. We also suggest the usage of eDNA/eRNA approach in urban aquatic samples like runoff in identifying the disease vectors/hosts inhabiting in locations which are not accessible easily. (c) 2021 Elsevier B.V. All rights reserved.

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