Metatranscriptomics reveals different features of methanogenic archaea among global vegetated coastal ecosystems

Vegetated coastal ecosystems (VCEs; i.e., mangroves, saltmarshes, and seagrasses) represent important sources of natural methane emission. Despite recent advances in the understanding of novel taxa and pathways associated with methanogenesis in these ecosystems, the key methanogenic players and the contribution of different substrates to methane formation remain elusive. Here, we systematically investigate the community and activity of methanogens using publicly available metatranscriptomes at a global scale together with our in-house metatranscriptomic dataset. Taxonomic profiling reveals that 13 groups of methanogenic archaea were transcribed in the investigated VCEs, and they were predominated by Methanosarcinales. Among these VCEs, methanogens exhibited all the three known methanogenic pathways in some mangrove sediments, where methylotrophic methanogens Methanosarcinales/Methanomassiliicoccales grew on diverse methyl compounds and coexisted with hydrogenotrophic (mainly Methanomicrobiales) and acetoclastic (mainly Methanothrix) methanogens. Contrastingly, the predominant methanogenic pathway in saltmarshes and seagrasses was constrained to methylotrophic methanogenesis. These findings reveal different archaeal methanogens in VCEs and suggest the potentially distinct methanogenesis contributions in these VCEs to the global warming. (c) 2021 Elsevier B.V. All rights reserved.

Automated summary

The study investigated methanogens in vegetated coastal ecosystems and found that mangroves have more diverse methane-producing archaea compared to saltmarshes and seagrasses, where methylotrophic methanogenesis is predominant. The research identified 13 groups of methanogenic archaea being transcribed in VCEs, with Methanosarcinales being the most predominant. This suggests potential distinct methane production contributions in different VCEs to global warming.