Journal
SCIENCE
Volume 374, Issue 6572, Pages 1247-1252Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.abj2327
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Funding
- NSF [IOS-1934388, IOS 1445025, IOS-1930101, PGRP-23020]
- NIH [R35GM136362]
- Human Frontier Science Program Organization [LT -000972/2018]
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A cell-resolution map of maize root was generated using single-cell RNA sequencing, revealing an alternative configuration of the tissue formative transcription factor SHR. The study shows that the SHR pathway controls the expansion of cortical tissue, impacting the anatomical complexity of plants.
Most plant roots have multiple cortex layers that make up the bulk of the organ and play key roles in physiology, such as flood tolerance and symbiosis. However, little is known about the formation of cortical layers outside of the highly reduced anatomy of Arabidopsis. Here, we used single-cell RNA sequencing to rapidly generate a cell-resolution map of the maize root, revealing an alternative configuration of the tissue formative transcription factor SHORT-ROOT (SHR) adjacent to an expanded cortex. We show that maize SHR protein is hypermobile, moving at least eight cell layers into the cortex. Higher-order SHR mutants in both maize and Setaria have reduced numbers of cortical layers, showing that the SHR pathway controls expansion of cortical tissue to elaborate anatomical complexity.
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