Article
Entomology
Cong Zhang, Wenfeng Hu, Zhuo Yu, Xian Liu, Jing Wang, Tianrong Xin, Zhiwen Zou, Bin Xia
Summary: This study investigated the transcriptional level of chitin synthase A (DcCHSA) in Diaphorina citri Kuwayama and its role in nymph development and eclosion. The findings revealed that DcCHSA was critical for the molting and survival of D. citri nymphs. This study provides valuable insights for the management of D. citri in China.
Article
Agronomy
Huan Wang, Xiaoshan Yang, Jin Liu, Jingwei Xu, Ruimin Zhang, Junnan Zheng, Bo Shen, Yan Sun, Dan Zhou
Summary: This study found that inhibiting the expression of Cpchsa gene can significantly reduce the reproductive capacity of female mosquitoes and affect the structure of their eggshells, providing a new approach for mosquito control.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agronomy
Jun Hu, He Cui, Mingsheng Hong, Yuxian Xia, Wenqing Zhang
Summary: This study constructed transgenic strains of Metarhizium anisopliae and demonstrated that the expression of dsRNA targeting the chitin synthase gene greatly enhanced the fungal virulence against the brown planthopper Nilaparvata lugens.
Article
Entomology
Runa Zhao, Chengxu Wu, Yingqin He, Chun Yu, Jianfeng Liu, Taisheng Li, Changyong Zhou, Wenlong Chen
Summary: The brown citrus aphid, Toxoptera citricida, is a major citrus pest causing significant damage. Host plant resistance is crucial for managing aphids, and this study assessed T. citricida resistance on seven common hosts. Results showed that the feeding behavior of T. citricida varied significantly among different host plants, with Eureka lemon exhibiting resistance. This study provides a theoretical basis for exploring aphid-resistant fruit tree resources.
Article
Biochemistry & Molecular Biology
Lin-Hong Jiang, Li-Li Mu, Lin Jin, Ahmad Ali Anjum, Guo-Qing Li
Summary: Chitin synthase 1 (HvCHS1) is responsible for chitin biosynthesis during ecdysis in Henosepilachna vigintioctopunctata. It is a potential amenable target gene and young larvae are more susceptible to dsRNA. Knockdown of HvCHS1 impairs larval development and transition stages, leading to stunting prepupae, deformed pupae, and misshapen adults, while also damaging gut integrity and trachea system development.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ardavan Farhadi, Ligang Lv, Jun Song, Yin Zhang, Shaopan Ye, Ning Zhang, Huaiping Zheng, Shengkang Li, Yueling Zhang, Mhd Ikhwanuddin, Hongyu Ma
Summary: By using RNA sequencing, the researchers identified the genetic basis underlying the eyestalk abnormality in novel mud crab hybrids, which is related to the differential expression of genes involved in chitin and cuticle synthesis.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Entomology
Meifeng Ren, Junjiao Lu, Daqi Li, Jing Yang, Yuying Zhang, Jinming Dong, Yanbing Niu, Xuguo Zhou, Xianhong Zhang
Summary: The black cutworm, Agrotis ipsilon, is a migratory insect that can cause significant economic damage to vegetables and grain crops worldwide. This study identified two key enzymes, AiCHS1 and AiCHS2, involved in chitin synthesis, which plays a crucial role in the growth and development of A. ipsilon.
JOURNAL OF ECONOMIC ENTOMOLOGY
(2023)
Article
Entomology
Bin Zeng, Fu-Rong Chen, Hao Sun, Yan Liu, Shun-Fan Wu, Chris Bass, Cong-Fen Gao
Summary: In this study, it was found that chitin synthase 1 and 2 (CsCHS1 and CsCHS2) play vital roles in the growth and development of the rice stem borer, Chilo suppressalis, suggesting their potential as insecticidal targets.
Article
Biochemistry & Molecular Biology
Dan Zhao, Zhao-rui Liu, Han Wu, Chao-ran Fu, Ya-zi Li, Xiu-jun Lu, Wei Guo
Summary: Chitin deacetylases are crucial for insect survival by catalyzing chitin N-deacetylation to produce chitosan, making them important targets for insecticidal drug development. The study identified putative Group I chitin deacetylase genes in Holotrichia parallela and demonstrated their essential role in cuticle organization, providing a basis for potential new control strategies.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Entomology
Gui-Jun Long, Xuan-Zheng Liu, Huan Guo, Meng-Qi Zhang, Lang-Lang Gong, Yun-Feng Ma, Youssef Dewer, Wu-Jia Mo, Ling-Wen Ding, Qin Wang, Ming He, Peng He
Summary: This study identified two chitin synthase genes (BgCHS1 and BgCHS2) in B. germanica for the first time and found that silencing these genes resulted in high mortality and abnormal development. These findings highlight their potential as gene targets for controlling urban health pests.
JOURNAL OF PEST SCIENCE
(2023)
Article
Entomology
Qingling Chen, Mingxu Sun, Hanyang Wang, Xiaohan Liang, Mingliang Yin, Tong Lin
Summary: Heortia vitessoides Moore is a leaf-eating pest that can cause the death of Aquilaria sinensis trees and economic losses. The chitin synthase B gene (Chsb) is crucial for the synthesis of chitin in the midgut peritrophic membrane of H. vitessoides. Inhibiting the expression of HvChsb leads to abnormal growth and development, as well as increased mortality rate. These findings provide insights for gene manipulation-based pest prevention and control.
Article
Entomology
Qiong Yao, Lin-Fa Quan, Shu Xu, Yi-Zhi Dong, Wen-Jing Li, Bing-Xu Chen
Summary: DFB is a potential ovicide for the control of Conopomorpha sinensisis, as it interferes with insect molting and egg hatching by affecting the expression of genes involved in chitin biosynthesis pathways. The altered gene expression leads to abnormal development, reduced chitin content, and modulated chitin distribution in newly hatched larvae.
Article
Agronomy
Shan Yang, Zhiwen Zou, Tianrong Xin, Shiyu Cai, Xi Wang, Huijie Zhang, Ling Zhong, Bin Xia
Summary: This study successfully targeted and silenced hexokinase gene in the Asian citrus psyllid using RNAi technology, resulting in impaired chitin synthesis, insect mortality, and abnormal phenotypes. These findings support the potential of using HK genes as a new target for citrus pest control.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Min Zhou, Qida Shen, Shasha Wang, Guoyong Li, Yan Wu, Caidi Xu, Bin Tang, Can Li
Summary: This study investigated the regulation of trehalose-6-phosphate synthase (TPS) in chitin metabolism in the brown planthopper. The results showed that inhibition of TPS expression affected the metabolism of trehalose and chitin in the insect. These findings provide a theoretical basis for pest control.
INSECT MOLECULAR BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Han Wu, Dan Zhao, Xiao-Chang Guo, Zhao-Rui Liu, Rui-Jun Li, Xiu-Jun Lu, Wei Guo
Summary: Chitin deacetylase (CDA) can accelerate the conversion of chitin to chitosan, influencing the mechanical properties and permeability of the cuticle structures and the peritrophic membrane (PM) in insects. Putative Group V CDAs were identified and characterized from beet armyworm larvae. The expression of these CDAs was found to be regulated by 20-hydroxyecdysone and juvenile hormone analog. Silencing of Group V CDAs resulted in compacted and evenly distributed intestinal wall cell layer, fragmented or disappeared vesicles, scarce PM structure, and loose and chaotic chitin microfilament structure. These results indicate the essential role of Group V CDAs in the growth and structuring of the midgut in beet armyworm larvae.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)