Article
Biochemical Research Methods
Helen Haug, Andreas T. Grasskamp, Satnam Singh, Andrea Strube, Tilman Sauerwald
Summary: This study evaluates the aroma properties of 16 whisky samples from Scotch and American origins using both chemical analysis and human sensory studies. The results show that whisky samples contain a wide range of aroma-active compounds, with eight significant aroma attributes identified. Furthermore, chemical analysis reveals over 200 mostly aroma-active volatiles in the whisky samples. A simple classification problem testing indicates that both sensory evaluation and analytical procedures are suitable for distinguishing between Scotch and American whisky.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Engineering, Environmental
Xueshu Li, Marco M. Hefti, Rachel F. Marek, Keri C. Hornbuckle, Kai Wang, Hans-Joachim Lehmler
Summary: This study investigated the distribution of PCBs and OH-PCBs in the human brain, finding higher levels of PCBs in adult brains and significant contributions of lower chlorinated PCBs to neurotoxicity in some donors.
ENVIRONMENTAL SCIENCE & TECHNOLOGY
(2022)
Article
Chemistry, Analytical
Ying Zhang, Bei Gao, Luis Valdiviez, Chao Zhu, Tara Gallagher, Katrine Whiteson, Oliver Fiehn
Summary: Stable isotope tracers are commonly used with gas chromatography coupled to mass spectrometry for enzyme activity and metabolic pathway studies. In this research, three commercially available instruments were used to analyze samples from a stable isotope labeling study on Rothia mucilaginosa metabolism. The study found that all three GC-MS instruments can be used for stable isotope tracing studies, with high-resolution GC-QTOF MS offering additional capabilities in identifying the chemical structures of unknown compounds.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
M. Cobo-Golpe, M. Ramil, R. Cela, I. Rodriguez
Summary: This article presents a fast and sensitive chiral analysis method for determining eight pharmaceuticals in wastewater and sludge samples. The compounds are separated using supercritical fluid chromatography combined with time-of-flight mass spectrometry detection. Methanol and ammonia are used as modifiers in the mobile phase. The method allows for the analysis of eight pairs of enantiomers in 15.5 minutes and has been successfully applied to the analysis of wastewater and sludge samples.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Review
Chemistry, Analytical
A. Fernez Asensio, E. Alvarez-Gonzalez, A. Rodriguez, L. M. Sierra, E. Blanco-Gonzalez
Summary: Dysregulation of TCA cycle enzymes leads to abnormal accumulation of oncometabolites, which contribute to cancer formation and progression. GC-MS and LC-MS are powerful analytical tools for accurate identification and determination of these metabolites.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemical Research Methods
Maarten Degreef, Eleanor M. Berry, Adrian Covaci, Kristof E. K. Maudens, Alexander L. N. van Nuijs
Summary: This study compares two easy-to-use screening methods for routine toxicological analysis. After analyzing 105 medico-legal case samples, it was found that both methods are effective for screening most compounds in toxicological analysis. The semi-quantitative analysis using the tMRM method with a small number of labeled internal standards yielded excellent results, while the semi-quantitative analysis using the QTOF method was more time-consuming.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2022)
Article
Food Science & Technology
Lua Vazquez, Daniel Armada, Maria Celeiro, Thierry Dagnac, Maria Llompart
Summary: The study aimed to develop a miniaturized and environmentally friendly method to obtain the phenolic profile of Galician honeys, and evaluate their total phenolic content and antioxidant activity. Liquid chromatography-tandem mass spectrometry was used to quantify 41 target phenolic compounds, allowing for classification of different honeys based on their botanical origin.
Review
Food Science & Technology
Xin-yue Zhang, Yuan Zhang, Yu Zhou, Zhi-fei Liu, Bin-bin Wei, Xue-song Feng
Summary: Melatonin (MLT) plays a crucial role in maintaining physiological functions and regulating metabolic processes. The global market share of MLT has been increasing due to its abundant functions. However, concerns arise regarding the quality issues and inappropriate or excessive application of MLT. Moreover, the detection of MLT is doubly difficult due to its photosensitivity, oxidation, complex substrate concentration, and trace levels. Therefore, it is essential to develop precise, sensitive, and stable extraction and detection methods. This study provides a comprehensive overview of the distribution, bioactivities, pretreatment, and analysis methods for MLT in food samples since 2010. Commonly used pretreatment methods include traditional techniques and novel ones such as solid-phase extraction, QuEChERS, microextraction by packed sorbent, solid phase microextraction, liquid phase microextraction, and more. Analysis methods include liquid chromatography coupled with different detectors, GC.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Chemistry, Analytical
David Izquierdo-Sandoval, David Fabregat-Safont, Leticia Lacalle-Bergeron, Juan V. Sancho, Felix Hernandez, Tania Portoles
Summary: The combination of GC with IMS-HRMS is considered a promising approach for monitoring (semi)volatile compounds in complex matrices. Utilizing soft ionization and ion mobility separation, it enables rapid, sensitive, and efficient wide-scope screening while improving (tentative) identifications. With the assessment of 264 organic pollutants, GC-APCI-IMS-HRMS demonstrated robust identification power in complex-matrix samples.
ANALYTICAL CHEMISTRY
(2022)
Article
Biochemical Research Methods
Delphine Zanella, Julien Guiot, Pierre-Hugues Stefanuto, Laurie Giltay, Monique Henket, Francoise Guissard, Beatrice Andre, Michel Malaise, Judith Potjewijd, Florence Schleich, Renaud Louis, Jean-Francois Focant
Summary: Analysis of volatile organic compounds in exhaled breath shows promise as a potential method for diagnosing systemic sclerosis, providing assistance for early diagnosis and prognosis of the disease.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Jan Beck, Martin Bruggemann, Dominik van Pinxteren, Hartmut Herrmann
Summary: In this study, a new analytical method using high resolution mass spectrometry was developed to detect complexing agents in atmospheric particles and rainwater. The method utilizes postcolumn complexation with FeCl3 to selectively detect the presence of complexing agents. The results show that the method can be applied to various matrices and can detect complexing agents at concentrations as low as 50 nM.
ANALYTICAL CHEMISTRY
(2022)
Article
Electrochemistry
Raluca-Ioana Stefan-van Staden, Bianca-Maria Tuchiu, Jacobus (Koos) Frederick van Staden, Paula Sfirloaga
Summary: A portable electrochemical platform that can detect methylprednisolone in two switchable modes, including square wave voltammetry and stochastic, has been developed. Both modes provide qualitative and quantitative analysis of the analyte. The platform showed linear concentration ranges of 1.00-1.00 x 10(3) mu mol L(-1) in square wave voltammetry mode and a much wider range of 1.00 x 10(-9)-1.00 x 10(4) mu mol L(-1) in stochastic mode. The limits of quantification were 1.00 fmol L(-1) for the stochastic mode and 1.00 mu mol L(-1) for the square wave voltammetry mode. The platform was successfully applied for the detection of methylprednisolone in real samples, with recoveries higher than 90.00%.
JOURNAL OF THE ELECTROCHEMICAL SOCIETY
(2023)
Review
Chemistry, Analytical
Albert Perez-Alcaraz, Francesc Borrull, Marta Calull, Carme Aguilar
Summary: The consumption of synthetic cathinones has been increasing in recent years due to its affordability and accessibility as an alternative to amphetamines. Developing procedures capable of detecting and monitoring their consumption is crucial, and urine has been widely used for this purpose as it is non-invasive and provides larger sample volumes than other biological matrices. Chromatographic techniques are the most reported approach for determining cathinones in urine samples.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2021)
Review
Medical Laboratory Technology
Kenichi Tamama
Summary: Drugs of abuse testing is commonly used in clinical and forensic settings, with urine being the preferred specimen type but other emerging types include saliva, sweat, hair, and meconium. Liquid chromatography-tandem mass spectrometry (LC-tandem-MS) has become the new gold standard for confirmatory drug testing due to its ability to analyze a wider range of analytes compared to gas chromatography-mass spectrometry (GC-MS). Technical advancements such as high-resolution mass spectrometry and miniature ambient ionization mass spectrometry have improved the speed and efficiency of drugs of abuse testing.
CLINICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Mark E. Jennings, Jay R. Silveira, Kimberly M. Treier, Paula B. Tracy, Dwight E. Matthews
Summary: A novel LC-MS method utilizing both reverse phase C18 column and PGC column was developed to retain both hydrophobic and hydrophilic peptides, addressing the issue of undetected hydrophilic peptides in a C18 nanocolumn setup.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Hannah E. Moore, Martin J. R. Hall, Falko P. Drijfhout, Robert B. Cody, Daniel Whitmore
Summary: Analysis of cuticular hydrocarbons (CHCs) in adult males and females of eleven species of flesh flies belonging to the genus Sarcophaga Meigen revealed species-specific and sexually dimorphic differences in CHC composition and quantity. CHC analysis can be used for identification and gender determination in insects.
SCIENTIFIC REPORTS
(2021)
Correction
Forestry
V. Deklerck, T. Mortier, N. Goeders, R. B. Cody, W. Waegeman, E. Espinoza, J. Van Acker, J. Van den Bulcke, H. Beeckman
WOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Biochemical Research Methods
Robert B. Cody
Summary: Organic acid salts have low volatility and are not ideal for DART-MS analysis, but addition of a strong acid can convert them to the free acid form to facilitate qualitative analysis.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Biochemical Research Methods
Robert B. Cody
Summary: This study identifies spurious peaks in mass spectra caused by detector oscillation or ringing, and successfully removes detector oscillation and electronic noise peaks from database spectra of pure compounds and mixtures, without loss of chemical information.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Biochemical Research Methods
Kirk R. Jensen, A. John Dane, Robert B. Cody
Summary: By combining a low-pressure gas chromatography column with the enhanced selected reaction monitoring switching speed of the short collision cell, the analysis time for pesticides can be dramatically reduced while maintaining a similar separation efficiency.
RAPID COMMUNICATIONS IN MASS SPECTROMETRY
(2022)
Article
Chemistry, Multidisciplinary
Allix M. Coon, A. John Dane, Gavin Setzen, Robert B. Cody, Rabi A. Musah
Summary: Earwax is a potential biological matrix for disease diagnostics, but its semisolid nature and high chemical complexity have made it difficult to investigate disease markers. Conventional analysis methods like GC-MS and LC-MS often yield unsatisfactory results due to the presence of many nonvolatile compounds, leading to the application of two-dimensional GC-MS for characterization without saponification. This study identified over 35 compounds in earwax for the first time and provided a foundation for future comparisons between healthy and diseased earwax.
Article
Medicine, Legal
Hannah Moore, Lena Lutz, Victoria Bernhardt, Falko P. Drijfhout, Robert B. Cody, Jens Amendt
Summary: Research has shown that social insects and Diptera have species-specific hydrocarbons on their cuticle, which can be a useful tool in Forensic Entomology. Analyzing the CHC chemical profiles of empty puparia from various fly species can help in species identification, as well as potentially indicate geographical differences. Different cuticular hydrocarbon profiles were found among the analyzed species, and even geographical locations could be determined from the chemical analysis.
INTERNATIONAL JOURNAL OF LEGAL MEDICINE
(2022)
Article
Biochemical Research Methods
Robert B. Cody, O. David Sparkman, Hannah Moore
Summary: The NIST Mass Spectral Search Program is useful for creating searchable databases of chromatograms, allowing for matching of chromatograms against the database. This method provides an alternative for managing simple chromatographic databases when commercial software is not available.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Biochemical Research Methods
Robert B. Cody
Summary: The study investigated the oxidation issue in nitrogen DART and proposed a solution to reduce oxidation phenomena by using a ceramic insulator cap with a narrow inner diameter.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Physics, Atomic, Molecular & Chemical
Mitsuo Takayama, Masaaki Ubukata, Keijiro Ohshimo, Kenji Nagatomo, Fuminori Misaizu
Summary: All observed fragment ions in the high-resolution electron ionization mass spectra of methyl stearate have been classified into three different series: methyl ester (CnHmO2), aliphatic hydrocarbon (CnHm), and acylium (CnHmO) ions. From the aliphatic hydrocarbon series, the compMcLR ion at m/z 224 and the compMcLR+1 ion at m/z 222 were identified as notable due to their intensity and rearrangement reactions. The fragmentation of the M.+ molecular ions was discussed based on the spin density evaluated by density functional theory (DFT) to determine the sites of unpaired electron and positive charge.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2023)
Article
Biochemical Research Methods
Robert B. Cody, Edgard O. Espinoza, Erin R. Price, Pamela J. McClure
Summary: By analyzing 3021 mass spectra from the ForeST database, it was found that there are significant differences in lignin peaks between hardwood angiosperms and coniferous gymnosperms. These differences can be used to accurately identify the source of finished wood products, providing support for enforcing trade laws.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Chemistry, Analytical
Krishnaja Tikkisetty, Taylor Filewood, Jeffrey Yan, Honoria Kwok, Pamela Brunswick, Robert Cody, Dayue Shang
Summary: In this study, a highly efficient oil identification method was developed using DART/TOFMS technology, which produced results similar to classical GC/FID and GC/MS/MS procedures in a much shorter time. This highlights the potential of DART/TOFMS as a new tool for oil spill forensic analysis.
ANALYTICAL METHODS
(2023)
Correction
Chemistry, Analytical
Roger W. Jones, Robert B. Cody, John F. McClelland
FORENSIC CHEMISTRY
(2021)