Investigation of the chemical characteristics and anticancer effect of plasma-activated water: The effect of liquid temperature

The liquid temperature during the preparation of plasma-activated water (PAW) seriously affects the PAW chemical characteristics and its biological effect. In this study, four different temperatures (4 degrees C, 25 degrees C, 40 degrees C, and 70 degrees C) of deionized water are selected as variable parameters to investigate the effect on PAW. The results show that the physicochemical properties and the concentration of reactive oxygen and nitrogen species of PAW are significantly reduced when the temperatures are higher than 25 degrees C; moreover, the above indexes are slightly decreased when the temperature is down to 4 degrees C. Furthermore, by comparing the results of cell viability and apoptosis, it is found that the PAW of 25 degrees C has a higher inactivation efficiency than other temperatures. Research on the temperature effect during PAW production is beneficial for tailoring the plasma-liquid system to enhance production efficiency.

Automated summary

The study found that PAW has a higher inactivation efficiency at 25 degrees Celsius, while the physicochemical properties and the concentration of reactive oxygen and nitrogen species of PAW significantly decrease when the temperature exceeds 25 degrees Celsius. Research on the temperature effect during PAW production is beneficial for optimizing production efficiency.