Journal
INNATE IMMUNITY
Volume 22, Issue 6, Pages 419-432Publisher
SAGE PUBLICATIONS LTD
DOI: 10.1177/1753425916651984
Keywords
Sepsis; M2 macrophage; inflammation; Trichinella spiralis; SOCS3
Categories
Funding
- Bureau of science and Information Technology, Guangzhou [201300000160]
- Provincial Scientific Project, Guangdong [2012B031800291]
- National Key Laboratory of Trauma, Burn and Compound Wounds, The 3rd Military Medical University [SKLKF201201]
Ask authors/readers for more resources
Sepsis is a serious clinical condition of excessive systemic immune response to microbial infection. The pro-inflammatory stage of sepsis is generally launched by innate cells such as macrophages. They release inflammatory cytokines, activate other immune cells and cause severe tissue/organ damage. In this study, we have revealed that recombinant Trichinella spiralis (TS) excretory-secretory protein (rTsP53) exhibited anti-inflammatory properties and rescued mice from LPS-induced endotoxemia, which is a common model for sepsis study, potentially through the induction of M2 macrophages. rTsP53 treatment significantly decreased inflammatory cytokines (IL-6, IFN- and TNF-) and increased IL-4, IL-10, IL-13 and TGF- secretion, both in circulation and in tissues. rTsP53 also induced the activation and infiltration of F4/80(+)CD163(+) macrophages to inflammatory tissues, increased M2 macrophage-related Arg1 and Fizz1 expression, and decreased M1 macrophage-related iNOS expression. PCR array showed that rTsP53 activated several genes that involve the survival of macrophages and also anti-inflammatory genes such as SOCS3. Together, our results show that rTsP53 activates M2 macrophages, which has strong anti-inflammatory potential to prevent LPS-induced lethal sepsis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available