Optimization of T-DNA configuration with UBIQUITIN10 promoters and tRNA–sgRNA complexes promotes highly efficient genome editing in allotetraploid tobacco

Optimizing the CRISPR/Cas9 system for genome editing in grape by using grape promoters

(2021) Chong Ren et al.   Horticulture Research

Rational Improvement of Rice Yield and Cold Tolerance by Editing the Three Genes OsPIN5b, GS3, and OsMYB30 With the CRISPR–Cas9 System

(2020) Yafei Zeng et al.   Frontiers in Plant Science

Development of a Highly Efficient Multiplex Genome Editing System in Outcrossing Tetraploid Alfalfa (Medicago sativa)

(2020) Tezera W. Wolabu et al.   Frontiers in Plant Science

Transcriptional silencing of 35S driven-transgene is differentially determined depending on promoter methylation heterogeneity at specific cytosines in both plus- and minus-sense strands

(2019) Wataru Matsunaga et al.   BMC PLANT BIOLOGY

Optimization of T-DNA architecture for Cas9-mediated mutagenesis in Arabidopsis

(2019) Baptiste Castel et al.   PLoS One

Efficient Targeted Mutagenesis in Apple and First Time Edition of Pear Using the CRISPR-Cas9 System

(2019) Aurélie Charrier et al.   Frontiers in Plant Science

A simple and reliable method for creating PCR-detectable mutants in Arabidopsis with the polycistronic tRNA–gRNA CRISPR/Cas9 system

(2019) Liangliang Hui et al.   ACTA PHYSIOLOGIAE PLANTARUM

High efficacy full allelic CRISPR/Cas9 gene editing in tetraploid potato

(2019) Ida Elisabeth Johansen et al.   Scientific Reports

Expression characterization of the herbicide tolerance gene Aryloxyalkanoate Dioxygenase (aad-1) controlled by seven combinations of regulatory elements

(2018) Delkin O. Gonzalez et al.   BMC PLANT BIOLOGY

CRISPOR: intuitive guide selection for CRISPR/Cas9 genome editing experiments and screens

(2018) Jean-Paul Concordet et al.   NUCLEIC ACIDS RESEARCH

Optimized paired-sgRNA/Cas9 cloning and expression cassette triggers high-efficiency multiplex genome editing in kiwifruit

(2018) Zupeng Wang et al.   PLANT BIOTECHNOLOGY JOURNAL

Simultaneous site-directed mutagenesis of duplicated loci in soybean using a single guide RNA

(2018) Yuhei Kanazashi et al.   PLANT CELL REPORTS

Characterization of a strong and constitutive promoter from the Arabidopsis serine carboxypeptidase-like gene AtSCPL30 as a potential tool for crop transgenic breeding

(2018) Pingping Jiang et al.   BMC BIOTECHNOLOGY

Optimization of CRISPR/Cas9 genome editing in cotton by improved sgRNA expression

(2018) Lu Long et al.   Plant Methods

How introns enhance gene expression

(2017) Orit Shaul   INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY

CRISPR-P 2.0: An Improved CRISPR-Cas9 Tool for Genome Editing in Plants

(2017) Hao Liu et al.   Molecular Plant

Synthetic introns help identify sequences in the 5′ UTR intron of the Glycine max polyubiquitin (Gmubi) promoter that give increased promoter activity

(2017) Trudi N. L. Grant et al.   PLANTA

High-efficiency CRISPR/Cas9 multiplex gene editing using the glycine tRNA-processing system-based strategy in maize

(2016) Weiwei Qi et al.   BMC BIOTECHNOLOGY

Efficiency and Inheritance of Targeted Mutagenesis in Maize Using CRISPR-Cas9

(2016) Jinjie Zhu et al.   Journal of Genetics and Genomics

CRISPR/Cas9 Platforms for Genome Editing in Plants: Developments and Applications

(2016) Xingliang Ma et al.   Molecular Plant

AnAgrobacterium-delivered CRISPR/Cas9 system for high-frequency targeted mutagenesis in maize

(2016) Si Nian Char et al.   PLANT BIOTECHNOLOGY JOURNAL

High efficiency of targeted mutagenesis in arabidopsis via meiotic promoter-driven expression of Cas9 endonuclease

(2016) Ayman Eid et al.   PLANT CELL REPORTS

RNAi-mediated suppression of dihydroflavonol 4-reductase in tobacco allows fine-tuning of flower color and flux through the flavonoid biosynthetic pathway

(2016) Sun-Hyung Lim et al.   PLANT PHYSIOLOGY AND BIOCHEMISTRY

Utilizing modified ubi1 introns to enhance exogenous gene expression in maize (Zea mays L.) and rice (Oryza sativa L.)

(2016) Yang-yang PAN et al.   Journal of Integrative Agriculture

Targeted genome modifications in soybean with CRISPR/Cas9

(2015) Thomas B Jacobs et al.   BMC BIOTECHNOLOGY

High-Efficiency Genome Editing in Arabidopsis Using YAO Promoter-Driven CRISPR/Cas9 System

(2015) Liuhua Yan et al.   Molecular Plant

Analyses of Ca2+dynamics using a ubiquitin-10 promoter-driven Yellow Cameleon 3.6 indicator reveal reliable transgene expression and differences in cytoplasmic Ca2+responses in Arabidopsis and rice (Oryza sativa) roots

(2015) Smrutisanjita Behera et al.   NEW PHYTOLOGIST

Development of germ-line-specific CRISPR-Cas9 systems to improve the production of heritable gene modifications inArabidopsis

(2015) Yanfei Mao et al.   PLANT BIOTECHNOLOGY JOURNAL

Efficient targeted mutagenesis in potato by the CRISPR/Cas9 system

(2015) Shaohui Wang et al.   PLANT CELL REPORTS

A multiplex CRISPR/Cas9 platform for fast and efficient editing of multiple genes in Arabidopsis

(2015) Zhengjing Zhang et al.   PLANT CELL REPORTS

Within leaf variation is the largest source of variation in agroinfiltration of Nicotiana benthamiana

(2015) Hany Bashandy et al.   Plant Methods

Comparison of CRISPR/Cas9 expression constructs for efficient targeted mutagenesis in rice

(2015) Masafumi Mikami et al.   PLANT MOLECULAR BIOLOGY

The enduring mystery of intron-mediated enhancement

(2015) Jenna E. Gallegos et al.   PLANT SCIENCE

Boosting CRISPR/Cas9 multiplex editing capability with the endogenous tRNA-processing system

(2015) Kabin Xie et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Targeted mutagenesis in soybean using the CRISPR-Cas9 system

(2015) Xianjun Sun et al.   Scientific Reports

CasOT: a genome-wide Cas9/gRNA off-target searching tool

(2014) A. Xiao et al.   BIOINFORMATICS

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases

(2014) S. Bae et al.   BIOINFORMATICS

A CRISPR/Cas9 toolkit for multiplex genome editing in plants

(2014) Hui-Li Xing et al.   BMC PLANT BIOLOGY

Simultaneous editing of three homoeoalleles in hexaploid bread wheat confers heritable resistance to powdery mildew

(2014) Yanpeng Wang et al.   NATURE BIOTECHNOLOGY

CRISPR/Cas9-Mediated Targeted Mutagenesis in the Liverwort Marchantia polymorpha L.

(2014) Shigeo S. Sugano et al.   PLANT AND CELL PHYSIOLOGY

Efficient Gene Editing in Tomato in the First Generation Using the Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated9 System

(2014) C. Brooks et al.   PLANT PHYSIOLOGY

Site-directed mutagenesis in Arabidopsis thaliana using dividing tissue-targeted RGEN of the CRISPR/Cas system to generate heritable null alleles

(2014) Youbong Hyun et al.   PLANTA

Progressive 35S promoter methylation increases rapidly during vegetative development in transgenic Nicotiana attenuata plants

(2013) Arne Weinhold et al.   BMC PLANT BIOLOGY

Targeted mutagenesis in rice using CRISPR-Cas system

(2013) Jin Miao et al.   CELL RESEARCH

Targeted Mutagenesis in Zea mays Using TALENs and the CRISPR/Cas System

(2013) Zhen Liang et al.   Journal of Genetics and Genomics

Multiplex and homologous recombination–mediated genome editing in Arabidopsis and Nicotiana benthamiana using guide RNA and Cas9

(2013) Jian-Feng Li et al.   NATURE BIOTECHNOLOGY

Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease

(2013) Vladimir Nekrasov et al.   NATURE BIOTECHNOLOGY

Targeted genome modification of crop plants using a CRISPR-Cas system

(2013) Qiwei Shan et al.   NATURE BIOTECHNOLOGY

Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in Arabidopsis, tobacco, sorghum and rice

(2013) Wenzhi Jiang et al.   NUCLEIC ACIDS RESEARCH

Targeted genome modification technologies and their applications in crop improvements

(2013) Kunling Chen et al.   PLANT CELL REPORTS

Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system

(2013) Khaoula Belhaj et al.   Plant Methods

GoldenBraid 2.0: A Comprehensive DNA Assembly Framework for Plant Synthetic Biology

(2013) A. Sarrion-Perdigones et al.   PLANT PHYSIOLOGY

The 5′UTR-intron of the Gladiolus polyubiquitin promoter GUBQ1 enhances translation efficiency in Gladiolus and Arabidopsis

(2012) Kathryn Kamo et al.   BMC PLANT BIOLOGY

Transcription Activator-Like Effector Nucleases Enable Efficient Plant Genome Engineering

(2012) Y. Zhang et al.   PLANT PHYSIOLOGY

Switchgrass (Panicum virgatum L.) polyubiquitin gene (PvUbi1 and PvUbi2) promoters for use in plant transformation

(2011) David GJ Mann et al.   BMC BIOTECHNOLOGY

Comparative and functional analysis of intron-mediated enhancement signals reveals conserved features among plants

(2011) G. Parra et al.   NUCLEIC ACIDS RESEARCH

A ubiquitin-10 promoter-based vector set for fluorescent protein tagging facilitates temporal stability and native protein distribution in transient and stable expression studies

(2010) Christopher Grefen et al.   PLANT JOURNAL

Promoter diversity in multigene transformation

(2010) Ariadna Peremarti et al.   PLANT MOLECULAR BIOLOGY

High frequency targeted mutagenesis in Arabidopsis thaliana using zinc finger nucleases

(2010) F. Zhang et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Analysis of promoters in transgenic barley and wheat

(2009) Agnelo Furtado et al.   PLANT BIOTECHNOLOGY JOURNAL

Strategies to mitigate transgene-promoter interactions

(2009) Loreta Gudynaite-Savitch et al.   PLANT BIOTECHNOLOGY JOURNAL