Article
Plant Sciences
Yu Zhou, Longlong Wang, Maria Carmen Rubio, Carmen Perez-Rontome, Yumiao Zhou, Yongmei Qi, Tao Tian, Weiqing Zhang, Qiuling Fan, Manuel Becana, Deqiang Duanmu
Summary: Legume nodules produce large amounts of heme for the synthesis of leghemoglobin and other hemoproteins. However, the mechanisms of heme homeostasis in nodules are still unknown.
Review
Plant Sciences
Jacob Banuelos, Esperanza Martinez-Romero, Noe Manuel Montano, Sara Lucia Camargo-Ricalde
Summary: Folates play multifunctional roles in plants, essential for cell division, nucleic acids and amino acid synthesis, as well as having a relevant role during symbiotic nitrogen fixation.
PHYSIOLOGIA PLANTARUM
(2021)
Review
Biochemistry & Molecular Biology
Nicholas J. Booth, Penelope M. C. Smith, Sunita A. Ramesh, David A. Day
Summary: Legumes form a symbiosis with rhizobia to access atmospheric nitrogen for plant growth. The process is energetically demanding, with a high demand for carbon supplied as sucrose. Transport pathways for malate and other organic acids to support nitrogen-fixing bacteroids have been identified, but further research is needed.
Article
Agronomy
Charlotte L. Thurston, Julie M. Grossman, Rebecca Fudge, Jude E. Maul, S. Mirsky, Nick Wiering
Summary: This study investigated the effects of cold temperatures on legume cover crop growth and symbiotic nitrogen fixation (SNF). The results showed that lower temperatures negatively affected legume cover crop growth and nodulation parameters, and the optimum temperature for SNF fell in the range of 15°C to 20°C. Winter conditions also had a negative impact on legume cover crop productivity, with hairy vetch showing the best growth response to spring warming.
Article
Biochemistry & Molecular Biology
Qinyi Ye, Fugui Zhu, Fanghao Sun, Tai-Cheng Wang, Jiale Wu, Peng Liu, Chen Shen, Jiangli Dong, Tao Wang
Summary: This study revealed the differentiation trajectories and interconnection of cells in root nodules using single-cell RNA sequencing. A spatial and functional cellular map was constructed, and it was found that apical meristematic cells in the nodules develop into symbiotic and un-symbiotic fate cells. In addition, the study discovered the specific functions and interrelationships of different cell clusters in the nodules.
Editorial Material
Plant Sciences
Philip S. Poole, Raphael Ledermann
Summary: Pinitol is transported passively across the symbiosome membrane in Lotus japonicus by the energy-independent polyol transporter LjPLT11. Its accumulation is crucial for maintaining osmotic balance in nodules. The disruption of symbiosome membranes, reduced nitrogen fixation, and increased production of reactive oxygen species in LjPLT11 RNAi lines indicate the importance of LjPLT11 in nodules.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Review
Plant Sciences
Samuel Minguillon, Manuel A. Matamoros, Deqiang Duanmu, Manuel Becana
Summary: This article discusses the roles of reactive oxygen, nitrogen, and sulfur species, as well as antioxidants, in the metabolic regulation and signaling of legume nodules. The article also raises several key questions, such as the need for new methodologies to detect and quantify these reactive molecules, the regulation of redox-active proteins, and the potential crosstalk between reactive oxygen, nitrogen, and sulfur species in nodules.
Article
Plant Sciences
Laurent Sauviac, Antoine Remy, Emeline Huault, Melanie Dalmasso, Theophile Kazmierczak, Marie-Francoise Jardinaud, Ludovic Legrand, Corentin Moreau, Bryan Ruiz, Anne-Claire Cazale, Sophie Valiere, Benjamin Gourion, Laurence Dupont, Veronique Gruber, Eric Boncompagni, Eliane Meilhoc, Pierre Frendo, Florian Frugier, Claude Bruand
Summary: This study identified hundreds of plant and bacterial genes associated with nodule senescence through dual plant-bacteria RNA sequencing, providing a valuable resource for exploring the mechanisms of nodule senescence. The study revealed that genes related to both plant and bacterial cell cycle and stress responses play important roles in nodule senescence, suggesting complex regulatory networks involved in this process.
PLANT CELL AND ENVIRONMENT
(2022)
Article
Microbiology
Quentin Nicoud, Florian Lamouche, Anais Chaumeret, Thierry Balliau, Romain Le Bars, Mickael Bourge, Fabienne Pierre, Florence Guerard, Erika Sallet, Solenn Tuffigo, Olivier Pierre, Yves Dessaux, Francoise Gilard, Bertrand Gakiere, Istvan Nagy, Attila Kereszt, Michel Zivy, Peter Mergaert, Benjamin Gourion, Benoit Alunni
Summary: The study reveals a low symbiotic efficiency between Bradyrhizobium diazoefficiens USDA110 and Aeschynomene afraspera, with a disconnection of terminal bacteroid differentiation features. This unique symbiotic relationship results in reduced bacterial viability under high stress conditions.
Article
Microbiology
Wen Feng Chen, Xiang Fei Meng, Yin Shan Jiao, Chang Fu Tian, Xin Hua Sui, Jian Jiao, En Tao Wang, Sheng Jun Ma
Summary: The development of bacteroids in the nodules of peanut and Sophora flavescens was investigated by using a single rhizobial strain, Bradyrhizobium arachidis. Bacteroids in peanut nodules were swollen and had a higher nitrogen fixation efficiency compared to nonswollen bacteroids in Sophora flavescens nodules. Transcriptome analysis revealed similar gene expression patterns in terms of nitrogen fixation and motility, but differences in urease activity and peptidoglycan biosynthesis.
MICROBIOLOGY SPECTRUM
(2023)
Article
Plant Sciences
Dhiraj Dokwal, Trevor B. Romsdahl, Daniel A. Kunz, Ana Paula Alonso, Rebecca Dickstein
Summary: Phosphorus (P) is an essential macronutrient for symbiotic nitrogen fixation (SNF) in legumes, and its deficiency can impair SNF activity and affect the accumulation of phosphate in roots and nodules. Under P deficiency conditions, membrane lipids in nodules exhibit changes, with alterations in the distribution of phospholipids (PL) species. Specific phosphatidylcholine (PC) species in nodules may play different roles in response to P stress, leading to heterogeneous distribution within nodules.
Article
Biochemistry & Molecular Biology
Hong-Rui Cao, Wen-Ting Peng, Miao-Miao Nie, Shuang Bai, Chun-Qu Chen, Qian Liu, Zi-Long Guo, Hong Liao, Zhi-Chang Chen
Summary: Symbiotic nitrogen fixation relies on the exchange of nutrients between legumes and rhizobia. This study reveals that magnesium plays a crucial role in this process, showing a positive correlation with carbon import and nitrogen export in soybean nodules. The researchers also identified two magnesium transporter genes that control the import of magnesium and the transport of carbon and nitrogen in the nodules.
Article
Plant Sciences
Ahmed Idris Hassen, Francina L. Bopape, Ansa van Vuuren, Abe Shegro Gerrano, Liesl Morey
Summary: This study aims to investigate the nodulation compatibility and symbiotic performance of twelve landraces of Bambara groundnut with five different Rhizobia species. Significant differences were observed among individual bambara genotypes and rhizobia strains in terms of nodule numbers and dry weight, as well as plant biomass. The findings of this study provide insights into the selection and development of appropriate low-cost rhizobia inoculants for sustainable production.
SOUTH AFRICAN JOURNAL OF BOTANY
(2023)
Review
Biochemistry & Molecular Biology
David A. Day, Penelope M. C. Smith
Summary: This review focuses on how iron is transported across the symbiosome membrane and accessed by the bacteroids within root nodules of legumes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Wen-Jing Cui, Biliang Zhang, Ran Zhao, Li-Xue Liu, Jian Jiao, Ziding Zhang, Chang-Fu Tian
Summary: This study utilized gene editing to improve compatibility between rhizobium and soybean. Transcriptomics revealed consistent lineage-dependent transcriptional profiles of core pathways, predating the divergence of legumes and rhizobia. Additionally, low-efficiency nodules exhibited impaired antioxidant activity and energy status, restricting nitrogen fixation activity.
Review
Plant Sciences
Manuel A. Matamoros, Manuel Becana
Summary: Legumes can reduce the demand for nitrogen fertilizers and contribute to sustainable agriculture by fixing nitrogen in symbiotic root nodules. However, global changes may increase exposure to abiotic stresses and negatively impact crop production. The complex regulation of whole plant physiology and nitrogen fixation in legumes under abiotic stress involves redox-based protein post-translational modifications, which play critical roles in plant acclimation and stress tolerance.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Biochemistry & Molecular Biology
Stefania Abbruzzetti, Alex J. Barker, Irene Villar, Carmen Perez-Rontome, Stefano Bruno, Giulio Cerullo, Cristiano Viappiani, Manuel Becana
Summary: The MtGlb1-2 gene produces four different protein splice forms, with very fast ligand binding rates and high reactivity. The hemoglobin encoded by this gene has remarkably high rates of binding to oxygen, nitric oxide, and nitrite.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Johan Quilbe, Leo Lamy, Laurent Brottier, Philippe Leleux, Joel Fardoux, Ronan Rivallan, Thomas Benichou, Remi Guyonnet, Manuel Becana, Irene Villar, Olivier Garsmeur, Barbara Hufnagel, Amandine Delteil, Djamel Gully, Clemence Chaintreuil, Marjorie Pervent, Fabienne Cartieaux, Mickael Bourge, Nicolas Valentin, Guillaume Martin, Loic Fontaine, Gaetan Droc, Alexis Dereeper, Andrew Farmer, Cyril Libourel, Nico Nouwen, Frederic Gressent, Pierre Mournet, Angelique D'Hont, Eric Giraud, Christophe Klopp, Jean-Francois Arrighi
Summary: Aeschynomene spp. utilize a unique symbiotic process for nitrogen-fixing nodulation, independent of traditional Nod factors and infection threads. Genetic studies in A. evenia reveal specific mechanisms in early and late nodulation steps, with key receptor-like kinase and symbiotic signaling genes necessary for triggering root and stem nodulation. This research highlights the importance of A. evenia as a model for understanding rhizobium-legume symbiosis.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Longlong Wang, Jianjun Liang, Yu Zhou, Tao Tian, Baoli Zhang, Deqiang Duanmu
Summary: In this study, 13 carbonic anhydrase (CA) genes were identified in the model legume Lotus japonicus, with three of them further characterized for their expression patterns during nodule development. Knockout experiments using CRISPR/Cas9 technology showed that the knocked-out genes were not essential for nitrogen fixation under normal symbiotic conditions. The diverse expression patterns and distributions of these genes suggest their potential functions in symbiotic nitrogen fixation, awaiting further investigation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Irene Villar, Maria C. Rubio, Laura Calvo-Begueria, Carmen Perez-Rontome, Estibaliz Larrainzar, Michael T. Wilson, Niels Sandal, Luis A. Mur, Longlong Wang, Brandon Reeder, Deqiang Duanmu, Toshiki Uchiumi, Jens Stougaard, Manuel Becana
Summary: Legumes express two major types of hemoglobins, symbiotic and non-symbiotic, with the latter categorized into three classes according to phylogeny and biochemistry. Knockout mutants show that all three phytoglobin classes are required for optimal development of Lotus japonicus. Mutants of two class 1 phytoglobins display different phenotypes, while the absence of a non-nodulated plant lacking LjGlb2-1 leads to delayed growth and changes in leaf metabolome. These results suggest the importance of phytoglobins in plant growth and development.
JOURNAL OF EXPERIMENTAL BOTANY
(2021)
Article
Microbiology
Hai-Feng Xu, Guo-Zheng Dai, Yu-Jie Wang, Chao Cheng, Jin-Long Shang, Ren-Han Li, Ke Liu, Deqiang Duanmu, Bao-Sheng Qiu
Summary: Through genome analysis of a subaerial desert cyanobacterium, it was found that it contains various phytochromes and cyanobacteriochromes that can sense multiple wavelengths of light. Additionally, a CBCR capable of acclimating to weak light environments at dawn was identified.
ENVIRONMENTAL MICROBIOLOGY
(2022)
Review
Plant Sciences
Samuel Minguillon, Manuel A. Matamoros, Deqiang Duanmu, Manuel Becana
Summary: This article discusses the roles of reactive oxygen, nitrogen, and sulfur species, as well as antioxidants, in the metabolic regulation and signaling of legume nodules. The article also raises several key questions, such as the need for new methodologies to detect and quantify these reactive molecules, the regulation of redox-active proteins, and the potential crosstalk between reactive oxygen, nitrogen, and sulfur species in nodules.
Article
Biochemistry & Molecular Biology
Zheng Li, Ahmed R. Henawy, Asmaa A. Halema, Qiuling Fan, Deqiang Duanmu, Renliang Huang
Summary: Rice domestication has improved agronomic traits but reduced genetic diversity. Dongxiang common wild rice, a wild rice species from northern China, provides a valuable genetic resource for rice breeding. In this study, a Burkholderia strain (BRDJ) isolated from the root of Dongxiang wild rice promoted rice growth and increased grain yield. The strain also showed differential effects on different rice varieties under nitrogen supply conditions. Genome analysis revealed genes related to nitrogen fixation and phytohormone and volatiles biosynthesis in BRDJ, which may explain its growth-promoting effects. BRDJ has the potential to be used as a biofertilizer for enhancing nitrogen use efficiency and overall growth in rice.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Plant Sciences
Longlong Wang, Tao Tian, Jianjun Liang, Runhui Li, Xian Xin, Yongmei Qi, Yumiao Zhou, Qiuling Fan, Guogui Ning, Manuel Becana, Deqiang Duanmu
Summary: LjNAC094 is involved in regulating nitrate-induced nodule senescence by controlling the expression of related genes. The study reveals the molecular mechanism of nodule senescence and provides a potential strategy to improve nitrogen fixation and stress tolerance of legumes.
Article
Plant Sciences
Yu Zhou, Longlong Wang, Maria Carmen Rubio, Carmen Perez-Rontome, Yumiao Zhou, Yongmei Qi, Tao Tian, Weiqing Zhang, Qiuling Fan, Manuel Becana, Deqiang Duanmu
Summary: Legume nodules produce large amounts of heme for the synthesis of leghemoglobin and other hemoproteins. However, the mechanisms of heme homeostasis in nodules are still unknown.
Article
Biotechnology & Applied Microbiology
Xue-Mei Yang, Jing-Hao Zhao, Xiao-Yu Xiong, Zhang-Wei Hu, Ji-Fen Sun, Hao Su, Yan-Jing Liu, Ling Xiang, Yong Zhu, Jin-Lu Li, Sadam Hussain Bhutto, Guo-Bang Li, Shi-Xin Zhou, Chi Li, Mei Pu, He Wang, Zhi-Xue Zhao, Ji-Wei Zhang, Yan-Yan Huang, Jing Fan, Wen-Ming Wang, Yan Li
Summary: The Arabidopsis RPW8.1 gene enhances disease resistance but compromises plant growth. It has been found that RPW8.1 constitutively enhances the expression of WRKY51 transcription factor and activates salicylic acid and ethylene signaling pathways. WRKY51, in turn, suppresses RPW8.1 expression, forming a feedback regulation loop.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Hannes Claeys, Eveline Neyrinck, Lies Dumoulin, Anne Pharazyn, Arne Verstichele, Laurens Pauwels, Michael L. Nuccio, Frederic Van Ex
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Melanie Ormancey, Bruno Guillotin, Camille Ribeyre, Clemence Medina, Nathanael Jariais, Helene San Clemente, Patrice Thuleau, Serge Plaza, Martina Beck, Jean-Philippe Combier
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Edgar Perez-Matas, Diego Hidalgo-Martinez, Elisabeth Moyano, Javier Palazon, Mercedes Bonfill
Summary: In this study, the production of paclitaxel was enhanced by overexpressing the bottleneck genes BAPT and DBTNBT in Taxus baccata cells. The transgenic cells showed significantly higher expression of these genes and improved taxane production compared to the wild type. Transcriptional profiling revealed that the GGPPS, TXS, and DBAT genes were most responsive to DBTNBT overexpression and the dual elicitation treatment.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Biying Dong, Dong Meng, Zhihua Song, Hongyan Cao, Tingting Du, Meng Qi, Shengjie Wang, Jingyi Xue, Qing Yang, Yujie Fu
Summary: This study identified the involvement of the citrate transporter CcMATE35 and the long noncoding RNA CcLTCS in aluminum stress response in pigeon pea. These findings suggest that the modules CcNFYB3-CcMATE35 and CcLTCS-CcCS jointly regulate the efflux and synthesis of citrate to enhance the resistance of pigeon pea under aluminum stress.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Review
Biotechnology & Applied Microbiology
Tien V. Vu, Ngan Thi Nguyen, Jihae Kim, Jong Chan Hong, Jae-Yean Kim
Summary: Prime editing technology uses an extended guide RNA to direct a fusion peptide to a specific location in the genome, enabling precise gene editing. This groundbreaking tool has potential in improving crop varieties, but efficiency limitations exist in certain plants.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Yan Xu, Jinyi Tan, Junxing Lu, Yuelin Zhang, Xin Li
Summary: Sclerotinia sclerotiorum causes white mold in economically important plants, and the discovery of SsGAP1 and SsRAS1/SsRAS2 genes as essential regulators of fungal development and virulence provides potential targets for controlling the disease.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Xiaoyang Chen, Chen Liu, Hailin Wang, Qi Liu, Yaping Yue, Yuhang Duan, Zhaoyun Wang, Lu Zheng, Xiaolin Chen, Yaohui Wang, Junbin Huang, Qiutao Xu, Yuemin Pan
Summary: This study identified a secreted protein, Uv1809, as a key virulence factor in Ustilaginoidea virens-rice interactions. Uv1809 inhibits rice immunity and promotes infection by targeting and enhancing rice histone deacetylase OsSRT2-mediated histone deacetylation. CRISPR-Cas9 edited ossrt2 mutants showed broad-spectrum resistance to rice pathogens, indicating its potential as a genetic resource for breeding disease resistance.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Weishuai Bi, Jing Liu, Yuanyuan Li, Ziwei He, Yongming Chen, Tingting Zhao, Xiangxiu Liang, Xiaodan Wang, Xiangzong Meng, Daolong Dou, Guangyuan Xu
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Shengjie You, Yu Wu, Wen Li, Xiaofeng Liu, Qinlan Tang, Fengkun Huang, Yan Li, Hsihua Wang, Mingchun Liu, Yang Zhang
Summary: In this study, a transcription factor called SlERF.G3-Like in tomato was found to play a role in regulating ethylene synthesis, cell wall degradation, and the flavonoid pathway. The researchers also discovered the interaction between SlERF.G3-Like and the master ripening regulator SlRIN, and increased the production of resveratrol derivatives through genetic manipulation. These findings provide insights into the coordination of fruit maturation and metabolic changes in tomatoes, and have implications for metabolic engineering.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
Article
Biotechnology & Applied Microbiology
Thomas R. Aalders, Mara de Sain, Fleur Gawehns, Nina Oudejans, Yoran D. Jak, Henk L. Dekker, Martijn Rep, Harrold A. van den Burg, Frank L. W. Takken
Summary: The TPL1 and TPL2 genes in tomato are closely associated with susceptibility to Fusarium wilt disease, and mutating these genes can enhance plant resistance to the disease.
PLANT BIOTECHNOLOGY JOURNAL
(2024)
