Journal
NUCLEIC ACIDS RESEARCH
Volume 50, Issue 3, Pages 1687-1700Publisher
OXFORD UNIV PRESS
DOI: 10.1093/nar/gkab1264
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Funding
- Department of Biotechnology (DBT), India [BT/COE/34/SP15219/2015]
- Ramalingaswami Fellowship from DBT, India [BT/RLF/Re-entry/23/2013]
- IISc-DBT partnership program
- Department of Science and Technology, India [DST/INT/POL/P-47/2020]
- PI grant
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This study identified type III TA systems belonging to the ToxIN family in different Escherichia coli strains and found them to be clustered into at least five distinct clusters. The crystal structure of the first E. coli ToxIN complex revealed the overall assembly of the protein-RNA complex, and isothermal titration calorimetry experiments showed that toxin forms a high-affinity complex with antitoxin RNA.
Toxin-antitoxin (TA) systems are proposed to play crucial roles in bacterial growth under stress conditions such as phage infection. The type III TA systems consist of a protein toxin whose activity is inhibited by a noncoding RNA antitoxin. The toxin is an endoribonuclease, while the antitoxin consists of multiple repeats of RNA. The toxin assembles with the individual antitoxin repeats into a cyclic complex in which the antitoxin forms a pseudoknot structure. While structure and functions of some type III TA systems are characterized, the complex assembly process is not well understood. Using bioinformatics analysis, we have identified type III TA systems belonging to the ToxIN family across different Escherichia coli strains and found them to be clustered into at least five distinct clusters. Furthermore, we report a 2.097 angstrom resolution crystal structure of the first E. coli ToxIN complex that revealed the overall assembly of the protein-RNA complex. Isothermal titration calorimetry experiments showed that toxin forms a high-affinity complex with antitoxin RNA resulting from two independent (5 ' and 3 ' sides of RNA) RNA binding sites on the protein. These results further our understanding of the assembly of type III TA complexes in bacteria.
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