Article
Virology
Liyun Jiang, Yuan Liu, Wenzhe Su, Yimin Cao, Qinlong Jing, Xinwei Wu, Zhicong Yang
Summary: The prevalent DENV-2 strains identified in the Guangzhou region are related to those in Southeast Asian countries, with the Malaysia/Indian subcontinent genotype prevailing. However, there has been no apparent genotype shift over the past 20 years. Episodic positive selection was detected at one site.
Letter
Immunology
Marta Giovanetti, Luiz Augusto Pereira, Gilberto A. Santiago, Vagner Fonseca, Maria Paquita Garcia Mendoza, Carla de Oliveira, Laise de Moraes, Joilson Xavier, Stephane Tosta, Hegger Fristch, Emerson de Castro Barbosa, Evandra Strazza Rodrigues, Dana Figueroa-Romero, Carlos Padilla-Rojas, Omar Caceres-Rey, Ana Flavia Mendonca, Fernanda de Bruycker Nogueira, Rivaldo Venancio da Cunha, Ana Maria Bispo de Filippis, Carla Freitas, Cassio Roberto Leonel Peterka, Carlos Frederico Campelo de Albuquerque, Leticia Franco, Jairo Andres Mendez Rico, Jorge L. Munoz-Jordan, Vinicius Lemes da Silva, Luiz Carlos Junior Alcantara
Summary: In this study, a cosmopolitan genotype of dengue virus serotype 2 was identified in a 56-year-old male patient from the state of Goias in Brazil using nanopore sequencing and phylogenetic analysis. The emergence of this genotype in Brazil highlights the need for risk assessment and surveillance to mitigate its epidemic potential.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Virology
Hirawati Deval, Sthita P. Behera, Awdhesh Agrawal, Rajeev Singh, Brijranjan Misra, Vanka Janardhan, Gajanan Patil, Kamlesh Sah, Niraj Kumar, Ravishankar Singh, Vijay P. Bondre
Summary: During the dengue outbreaks in 2015 to 2016 in Uttar Pradesh and Bihar, only DEN-2 serotype was prevalent, with DV-2 being the main circulating serotype. Genetic analysis revealed the presence of lineages I and III of DV-2 cosmopolitan genotype in 2015, and lineage II in 2016, based on full-length envelope (E) protein sequence derived from patient's sera and DV isolates. The phylogenetic analysis showed a genetic relationship between these DV-2 strains and recent DV-2 genotypes reported in DEN outbreaks in north India, indicating the circulation of cosmopolitan genotype of DV-2 in eastern Uttar Pradesh and western Bihar.
JOURNAL OF MEDICAL VIROLOGY
(2021)
Article
Microbiology
Hua Sun, Wenwu Yao, Abubakar Siddique, Fan He, Min Yue
Summary: This study aimed to investigate the genetic diversity and molecular evolution of the dengue virus in Hangzhou, China, during and after a dengue fever epidemic. The results showed that the virus strains from 2017 were imported from Singapore, Malaysia, and Thailand, while local strains from 2018 and 2019 were closely associated with Guangzhou isolates, indicating within-country transmission. Various amino acid substitutions and recombination events were observed.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Microbiology
Marcus Vinicius de Franca Cirilo, Shahab Zaki Pour, Viviane de Fatima Benedetti, Jessica Pires Farias, Mayanna Moreira Costa Fogaca, Rafael da Conceicao Simoes, Paloma Oliveira Vidal, Alexander Birbrair, Paolo Marinho de Andrade Zanotto, Wilson Barros Luiz, Jaime Henrique Amorim
Summary: This study conducted research in two main cities of Western Bahia, Brazil, and used molecular biology assays to detect various mosquito-borne viruses, including CHIKV, DENV, and ZIKV. The results showed the co-circulation of these viruses in the region, highlighting the importance of laboratory diagnosis in monitoring disease transmission.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Virology
Lijuan Qiu, Xinwei Huang, Jia Luo, Yujiao Zhao, Shan Hong, Xiaodan Wang, Kai Feng, Yue Pan, Qiangming Sun
Summary: The study investigates the risk of cross infection and severity of dengue virus in BALB/c mice, particularly focusing on the impact of maternal immunity and heterotypic infection. The results suggest that maternal immunity enhances subsequent heterotypic infection and can lead to severe disease. This study has implications for understanding cross infection and vaccine development.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Virology
Maria Celeste Torres, Marcos Cesar Lima de Mendonca, Cintia Damasceno dos Santos Rodrigues, Vagner Fonseca, Mario Sergio Ribeiro, Ana Paula Brandao, Rivaldo Venancio da Cunha, Ana Isabel Dias, Lucy Santos Vilas Boas, Alvina Clara Felix, Maira Alves Pereira, Luzia Maria de Oliveira Pinto, Anavaj Sakuntabhai, Ana Maria Bispo de Filippis
Summary: The study found significant differences in viral population structure among different clinical classifications of Dengue virus serotype 2 circulating in Brazil, driven mainly by different infection times and selection pressures rather than clinical outcomes. However, diversity in the NS2B gene was found to be constrained, regardless of clinical outcome and infection time. High frequency of non-synonymous intrahost single-nucleotide variants were identified among cases with warning signs and severe cases, but no single variant appeared to characterize cases of greater severity independently.
Article
Virology
Priya Verma, Sayan Banerjee, Upasana Baskey, Supradip Dutta, Sagnik Bakshi, Raina Das, Sandip Samanta, Shanta Dutta, Provash C. Sadhukhan
Summary: This study aims to investigate the circulating dengue serotypes and their clinicopathological associations among pediatric patients in Kolkata, India. It was found that all four serotypes co-circulated, with DENV2 being the major strain. The study also observed changes in trends of clinical manifestations and liver function profiles during different phases of dengue fever.
JOURNAL OF MEDICAL VIROLOGY
(2022)
Article
Immunology
Su-Jhen Hung, Huey-Pin Tsai, Ya-Fang Wang, Wen-Chien Ko, Jen-Ren Wang, Sheng-Wen Huang
Summary: This study investigates the association between the detected frequencies/times of SNVs/DVGs in dengue patients and severe disease. The results show that certain SNVs and DVGs are significantly different between patients with dengue fever and those with severe dengue, and a machine learning model can successfully discriminate severe patients. These findings expand our understanding of the intrahost viral population in dengue pathogenesis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Virology
Mya Myat Ngwe Tun, Kishor Pandey, Takeshi Nabeshima, Aung Kyaw Kyaw, Mandira Adhikari, Sandra Kendra Raini, Shingo Inoue, Shyam Prakash Dumre, Basu Dev Pandey, Kouichi Morita
Summary: Dengue virus (DENV) is increasingly prevalent in Nepal, with the most common serotype being DENV-2. Genomic analysis showed multiple amino acid substitutions in the E, NS3, and NS5 genes, and DENV-2 isolates in Nepal were classified into Asian and Indian clades. Further research on different DENV serotypes is needed to understand the epidemics in Nepal.
Article
Infectious Diseases
Esther Annan, Jailos Lubinda, Jesus Trevino, William Messer, Dina Fonseca, Penghua Wang, Jurgen Pilz, Benjamin Lintner, Aracely Angulo-Molina, Ana L. Gallego-Hernandez, Ubydul Haque
Summary: Pathogen strain diversity plays a crucial role in epidemic trajectories. In this study, the impact of bioclimatic factors on the spatial distribution of dengue virus serotypes was examined in Mexico. Municipality-level environmental suitability maps were developed using climatic variables and maximum entropy modeling. The results highlighted the importance of temperature, precipitation, and humidity in determining the suitability for different DENV serotypes. Ongoing surveillance and monitoring of bioclimatic parameters are recommended to track potential changes in DENV serotype distribution.
TRANSBOUNDARY AND EMERGING DISEASES
(2023)
Article
Chemistry, Analytical
Kanaporn Poltep, Emi E. Nakayama, Tadahiro Sasaki, Takeshi Kurosu, Yoshiki Takashima, Juthamas Phadungsombat, Nathamon Kosoltanapiwat, Borimas Hanboonkunupakarn, Sarin Suwanpakdee, Hisham A. Imad, Narinee Srimark, Chiaki Kitamura, Atsushi Yamanaka, Akio Okubo, Tatsuo Shioda, Pornsawan Leaungwutiwong
Summary: The research team successfully developed serotype-specific immunochromatography systems for all four DENV serotypes, showing high specificity and sensitivity. These systems can rapidly detect DENV with good performance in laboratory and clinical validation. In DENV-endemic areas, these assay systems could accelerate both infection diagnosis and epidemiologic studies.
Article
Immunology
S. Gowri Sankar, T. Mowna Sundan, A. Alwin Prem Anand
Summary: The study aimed to identify the molecular characteristics of DENV during the 2017 outbreak in Tamil Nadu, India, and investigate the role of inflammatory cytokines in different serotypes and dengue severity. Different cytokine expression profiles were observed in various serotypes, with DENV-4 playing a significant role in disease severity as the predominant serotype.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Parasitology
Mengmeng Ma, Sean Wu, Zhenjian He, Lihong Yuan, Zhijun Bai, Liyun Jiang, John Marshall, Jiahai Lu, Zhicong Yang, Qinlong Jing
Summary: The study found that new genotype invasion was closely correlated with Dengue fever outbreaks, and replacement and co-circulation of different genotypes were also observed.
PARASITES & VECTORS
(2021)
Article
Microbiology
Pedro M. Palermo, Antonio de la Mora-Covarrubias, Jeanette Orbegozo, Jessica A. Plante, Kenneth S. Plante, Florinda Jimenez-Vega, Douglas M. Watts
Summary: Dengue fever is the most important human arboviral disease globally, and sporadic outbreaks have been reported in urban communities along the Texas-Mexico border since 1980. A recent case in Ciudad Juarez, Mexico, involved a local transmission of DENV-1, as confirmed by virus isolation and sequencing.
Article
Public, Environmental & Occupational Health
Astri Nur Faizah, Daisuke Kobayashi, Yoshihide Maekawa, Michael Amoa-Bosompem, Shifa Fauziyah, Kris Cahyo Mulyatno, Sri Subekti, Etik Ainun Rohmah, Maria Inge Lusida, Yasuko Mori, Kozue Miura, Kazuhiro Hirayama, Haruhiko Isawa, Kyoko Sawabe
Summary: In Indonesia's Tabanan Regency, Bali Island, the study found that Culex vishnui mosquitoes are the main vectors transmitting Japanese encephalitis virus, with genetic analysis revealing it to be genotype IV. This indicates that the virus continues to circulate in restricted areas between swine-mosquito vectors.
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)
Article
Microbiology
Yanzhao Zhang, Seiya Ozono, Takuya Tada, Minoru Tobiume, Masanori Kameoka, Satoshi Kishigami, Hideaki Fujita, Kenzo Tokunaga
Summary: MARCH8 is an antiviral factor that can inhibit viral envelope glycoproteins by recognizing their cytoplasmic lysine residues, resulting in lysosomal degradation. This study reveals the broad-spectrum inhibitory activity of MARCH8 against various viral envelope glycoproteins.
MICROBIOLOGY SPECTRUM
(2022)
Article
Multidisciplinary Sciences
Masahiro Urushidani, Akira Kawayoshi, Tomohiro Kotaki, Keiichi Saeki, Yasuko Mori, Masanori Kameoka
Summary: This study attempted to inactivate SARS-CoV-2 and influenza A virus adhering to environmental surfaces using dry fogging with hypochlorous acid solution and hydrogen peroxide solution. The results showed that SARS-CoV-2 was more resistant to the virucidal effects of these disinfectants than influenza A virus, requiring higher concentrations or longer contact times for inactivation.
Article
Biotechnology & Applied Microbiology
Hasriaton Padmi, Viol Dhea Kharisma, Arif Nur Muhammad Ansori, Mada Triandala Sibero, Muhammad Hermawan Widyananda, Md Emdad Ullah, Olga Gumenyuk, Svetlana Chylichcova, Natalia Bratishko, Eka Sunarwidhi Prasedya, Teguh Hari Sucipto, Rahadian Zainul
Summary: This study predicts the potential of macroalgae compounds as SARS-CoV-2 antiviral drugs through in silico approach. The results show that siphonaxanthin, a macroalgae compound, has strong binding energy to ACE2 receptor. Macroalgae compounds are predicted to have potential as SARS-CoV-2 antiviral drugs.
JOURNAL OF PURE AND APPLIED MICROBIOLOGY
(2022)
Article
Microbiology
Minori Takaichi, Kayo Osawa, Ryohei Nomoto, Noriko Nakanishi, Masanori Kameoka, Makiko Miura, Katsumi Shigemura, Shohiro Kinoshita, Koichi Kitagawa, Atsushi Uda, Takayuki Miyara, Ni Made Mertaniasih, Usman Hadi, Dadik Raharjo, Ratna Yulistiani, Masato Fujisawa, Kuntaman Kuntaman, Toshiro Shirakawa
Summary: This study confirms the increase in antibiotic resistance in non-typhoidal Salmonella enterica (NTS) in Indonesia. The NTS strains isolated from chicken meat showed high resistance to multiple antibiotics and had many virulence factors. Particularly, a serotype called S. Schwarzengrund was frequently detected as multi-antimicrobial resistant and had a high prevalence of virulence genes.
Article
Medicine, General & Internal
Soegeng Soegijanto, Siti Churrotin, Teguh H. Sucipto, Novina Aryanti
Summary: This study focused on the clinical manifestation of dengue virus infection in Surabaya, Indonesia from January 1 to June 30, 2018. The diagnosis of cases was based on the 2009 criteria set by the World Health Organization.
GAZZETTA MEDICA ITALIANA ARCHIVIO PER LE SCIENZE MEDICHE
(2022)
Article
Biochemical Research Methods
Atsushi Yamanaka, Pimploy Rattanaamnuaychai, Mami Matsuda, Ryosuke Suzuki, Jun Shimizu, Tatsuo Shioda, Kazuo Miyazaki
Summary: In this study, a rapid assay system was established to evaluate ADE activity in dengue-seropositive samples using single round infectious particles (SRIPs). Two assay protocols were introduced and both could provide valuable information regarding the risk assessment for severe diseases.
JOURNAL OF VIROLOGICAL METHODS
(2023)
Article
Multidisciplinary Sciences
Akina Nakamura, Tomohiro Kotaki, Yurie Nagai, Shunta Takazawa, Kenzo Tokunaga, Masanori Kameoka
Summary: Replicon RNA (repRNA) vaccine is a promising vaccine platform that can self-replicate and act as an adjuvant, generating robust immunity. This study successfully constructed and evaluated a repRNA vaccine that induced high levels of T-cell response but had minimal impact on antibody response. Future work should focus on optimizing vaccine administration methods and improving S protein expression to enhance the immune-inducing ability of the repRNA vaccine.
Article
Immunology
Tomohiro Kotaki, Yurie Nagai, Atsushi Yamanaka, Eiji Konishi, Masanori Kameoka
Summary: This study focused on developing a Japanese encephalitis (JE) vaccine that does not induce antibody-dependent enhancement (ADE) of dengue virus (DENV) infection. By introducing a mutation at the 106th amino acid residue of the JEV E protein, the modified JE vaccine was able to significantly reduce the production of cross-reactive ADE-prone antibodies against DENV.
Article
Immunology
Siti Qamariyah Khairunisa, Ni Luh Ayu Megasari, Shuhei Ueda, Tomohiro Kotaki, Afif Nurul Hidayati, Nasronudin Nasronudin, Masanori Kameoka
Summary: This study investigated the distribution of HIV-1 subtypes and the emergence of drug resistance mutations (DRMs) in Makassar, South Sulawesi, Indonesia. CRF01_AE was identified as the predominant circulating recombinant form (CRF) in this region, along with subtype B and recombinant viruses containing CRF02_AG and/or subtype B gene fragments. DRMs against non-nucleoside reverse transcriptase inhibitors were found among ART-experienced individuals, while no transmitted drug resistance was observed in ART-naive individuals.
AIDS RESEARCH AND HUMAN RETROVIRUSES
(2023)
Article
Agriculture, Dairy & Animal Science
Eduardus Bimo Aksono, Kania Rifa Iradatya, Teguh Hari Sucipto, Nur Syamsiatul Fajar, Wiwik Misaco Yuniarti
Summary: This study conducted a comprehensive analysis of viruses in cats and found a relationship between SARS-CoV-2 and FECV, a virus that infects cats. This is an important finding for cat owners. However, further laboratory studies are needed to support this finding.
Article
Immunology
Chie Aoki-Utsubo, Puguh Indrasetiawan, Kento Fukano, Masamichi Muramatsu, Nina Artanti, Muhammad Hanafi, Hak Hotta, Masanori Kameoka
Summary: In this study, a plant-derived polyphenolic bioflavonoid called amentoflavone was identified as a new anti-HBV compound. It demonstrated dose-dependent inhibition of HBV infection in susceptible cells by targeting the viral entry process.
MICROBIOLOGY AND IMMUNOLOGY
(2023)
Article
Infectious Diseases
Budi Utomo, Chow Khuen Chan, Ni Made Mertaniasih, Soedarsono Soedarsono, Shifa Fauziyah, Teguh Hari Sucipto, Febriana Aquaresta, Dwinka Syafira Eljatin, I. Made Dwi Mertha Adnyana
Summary: This study examines the recent epidemiology of tuberculosis and COVID-19 in East Java Province, Indonesia in 2020. The results reveal a high incidence rate of tuberculosis and a relatively lower incidence rate of COVID-19. The search for active cases and preventive promotion was incomplete, highlighting the need for inter-sectoral collaboration.
TROPICAL MEDICINE AND INFECTIOUS DISEASE
(2022)
Meeting Abstract
Public, Environmental & Occupational Health
Astri Nur Faizah, Daisuke Kobayashi, Maekawa Yoshihide, Michael Amoa-Bosompem, Shifa Fauziyah, Kris Cahyo Mulyatno, Sri Subekti, Etik Ainun Rohmah, Maria Inge Lusida, Yasuko Mori, Kozue Miura, Kazuhiro Hirayama, Haruhiko Isawa, Kyoko Sawabe
AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE
(2021)
