Article
Chemistry, Analytical
Fangteng Song, Chao Wang, Chunhua Wang, Jianwei Gao, Hong Liu, Yu Zhang, Lin Han
Summary: This Feature discusses the working fundamentals, advanced strategies, and future outlook and challenges of enrichment-detection integrated biosensors for exosome profiling.
ANALYTICAL CHEMISTRY
(2021)
Review
Engineering, Biomedical
Juan Li, Yanru Li, Peilong Li, Yi Zhang, Lutao Du, Yunshan Wang, Chengpeng Zhang, Chuanxin Wang
Summary: Exosomes, as nanoscale extracellular vesicles, are widely distributed in various biological fluids and can carry tumor-specific molecules. The clinical translation of exosomes remains challenging due to the requirement of large quantities of samples, insufficiency of traditional techniques, and complex isolation methods. Surface-enhanced Raman scattering (SERS) technology provides a sensitive and multiplexed detection method for exosomes without the need for large samples. In this review, SERS-based exosome detection in cancer diagnosis is discussed, providing valuable insights for early cancer detection.
ACTA BIOMATERIALIA
(2022)
Article
Chemistry, Applied
Ni Tan, Zui Ding, Can Chen, Yincheng Yang, Leqing He, Sen Liao, Lijie Liu, Duoduo Wang, Qiaorong Ye
Summary: For the first time, a new pH/redox dual stimulus-responsive formononetin imprinting material was successfully prepared by combining N,N'-bisacryloylcystamine containing disulfide bond as the cross-linking agent with molecularly imprinted technology. The material showed excellent drug-loading property and selectivity to formononetin, and had sensitivity to both pH and redox environments. The repeated regeneration experiment demonstrated its stability and reusability.
REACTIVE & FUNCTIONAL POLYMERS
(2022)
Article
Biophysics
Liang Zhao, Hong Wang, Jun Fu, Xia Wu, Xiao-ye Liang, Xue-yu Liu, Xue Wu, Liang-liang Cao, Zhen-yu Xu, Ming Dong
Summary: The study introduces an automated centrifugal microfluidic disc system combined with a novel aptamer fluorescence system to efficiently isolate and purify exosomes for detecting cancer with high accuracy in clinical samples. Compared to traditional techniques, this method is cost-effective, rapid, high in purity, sensitivity, and specificity.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Vojtech Vinduska, Caleb Edward Gallops, Ryan O'Connor, Yongmei Wang, Xiaohua Huang
Summary: The study introduced a facile method for exosome surface protein detection using quantum dot coupled with immunomagnetic capture and enrichment, which can specifically detect different surface markers on exosomes from different cancer cell lines. The clinical potential was demonstrated with pilot plasma samples using HER2-positive breast cancer as the disease model.
Article
Engineering, Biomedical
Lin Li, Lili Zhang, Katelynn C. Montgomery, Li Jiang, Christopher J. Lyon, Tony Y. Hu
Summary: Exosomes are membrane-defined extracellular vesicles (EVs) that act as important vehicles for intercellular communication and can serve as circulating biomarkers for disease diagnosis and prognosis. They play a key role in tumor metastasis, are abundant in biofluids, and stabilize the biomarkers they carry, thus improving cancer detection, treatment monitoring, and cancer staging/prognosis. However, the lack of sensitive/specific biomarkers and isolation/enrichment technologies has hindered the clinical translation of exosomes.
MATERIALS TODAY BIO
(2023)
Article
Chemistry, Applied
Brenda G. Molina, Roshan B. Vasani, Karyn L. Jarvis, Elaine Armelin, Nicolas H. Voelcker, Carlos Aleman
Summary: We have developed a flexible system that can release antibiotic and act as a pH sensor under electrostimulation. This system has dual pH and electro-responsiveness and has been tested on bacterial strains for its effectiveness.
REACTIVE & FUNCTIONAL POLYMERS
(2022)
Article
Biochemistry & Molecular Biology
Sirilak Wangngae, Kantapat Chansaenpak, Tunyawat Khrootkaew, Rung-Yi Lai, Anyanee Kamkaew
Summary: In this study, pH-sensitive and hypoxia-responsive probes based on vanillin and the indole ring (Val-Hcys) were synthesized. The fluorescence of the probes can be activated at acidic pH and significantly increased when incubated with hypoxic cells. These probes tend to accumulate in lysosomes and mitochondria, and surprisingly, improve cell viability in hypoxic conditions.
BIOORGANIC CHEMISTRY
(2022)
Article
Chemistry, Analytical
Daniela A. Oliveira, Suleiman Althawab, Eric S. McLamore, Carmen L. Gomes
Summary: A label-free and rapid electrochemical biosensor for detecting Listeria monocytogenes was developed using a one-step simultaneous sonoelectrodeposition of platinum and chitosan. The sensor showed optimized deposition on the electrode surface, leading to significantly increased electroactive surface area. Functionalized with aptamers selective for L. monocytogenes capture, the aptasensor demonstrated high selectivity and sensitivity in detecting the bacteria quickly and efficiently.
Article
Biochemistry & Molecular Biology
Nima Sayyadi, Sareh Zhand, Sajad Razavi Bazaz, Majid Ebrahimi Warkiani
Summary: The study proposes a novel strategy of using Affibody functionalized microbeads (AffiBeads) for exosome detection with a high degree of efficiency. Results showed that the Affibody probe had a detection limit of 15.6 ng exosomes per mL (~12 exosomes per AffiBead), providing a basis for early-stage cancer diagnosis by sensitive detection of low expression level markers on tumor-derived exosomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Polymer Science
Nour M. AlSawaftah, Nahid S. Awad, William G. Pitt, Ghaleb A. Husseini
Summary: Stimuli-responsive nanoparticles have shown promising potential in overcoming the limitations of conventional chemotherapy. pH-sensitive nanoparticles, in particular, have proven to be highly efficient in delivering drugs to tumors compared to conventional nanoparticles. This review summarizes the different strategies used to develop pH-sensitive nanoparticles for cancer therapy.
Article
Chemistry, Multidisciplinary
Song Zhang, Jia Lv, Peng Gao, Qiuyu Feng, Hui Wang, Yiyun Cheng
Summary: This study presented a dendrimer engineered with a high density of 4-diethylaminophenyl groups on the surface, which could efficiently assemble with cargo proteins in serum solutions, enabling intracellular protein delivery. The dendrimer exhibited pH-responsive disassembly behavior after cell internalization, successfully delivering various cargo proteins into living cells with maintained bioactivity. This provides a convenient and efficient strategy for designing polymers with high serum-tolerance in cytosolic protein delivery.
Article
Chemistry, Analytical
Samran Durrani, Zihuayuan Yang, Jie Zhang, Zihao Wang, Haiyan Wang, Faran Durrani, Fu-Gen Wu, Fengming Lin
Summary: This article reports a fluorescent sensor that can directly monitor changes in nucleus pH, which is of great significance in understanding nucleus-related biological events.
Article
Chemistry, Analytical
Sevketcan Sarikaya, Frank Gardea, Jeffrey T. Auletta, Jamshid Kavosi, Alex Langrock, David M. Mackie, Mohammad Naraghi
Summary: Polymeric artificial muscles offer high actuation stroke and lightweight nature, but many rely on large temperature changes which may cause polymer degradation. This study explores athermal pH-responsive artificial muscles made of aligned and coiled fibers to amplify actuation stroke and work capacity. The coiled pH-responsive fibers show significant improvements in contractive actuation stroke and work capacity compared to their aligned counterparts.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Physical
Chaolei Hu, Yimeng Sun, Gil van Wissen, Yitong Peng, Andrij Pich
Summary: In this study, visible light- and temperature-responsive poly(N-vinylcaprolactam) microgels decorated by donor-acceptor Stenhouse adducts (DASAs) were synthesized for the first time using a robust and straightforward post-modification synthesis route. The microgels exhibited homogeneous distributions and temperature responsiveness in aqueous solutions. The photoswitching behaviors of the microgels in different solvents were systematically investigated, and their size in aqueous solutions could be modulated by visible light irradiation. Moreover, the microgels showed potential application in the photostimulated delivery of hydrophobic drugs.
CHEMISTRY OF MATERIALS
(2022)