Article
Biochemistry & Molecular Biology
Kamil Rozniakowski, Krystian Galecki, Joanna Wietrzyk, Beata Filip-Psurska, Justyna Fraczyk, Zbigniew J. Kaminski, Beata Kolesinska
Summary: The study aimed to find N-methylated analogs that inhibit amylin aggregation. N-methylated peptides showed varying abilities to inhibit aggregation in different amylin fragments.
CHEMISTRY & BIODIVERSITY
(2021)
Article
Biochemistry & Molecular Biology
Andrzej Fraczyk, Lukasz Janczewski, Joanna Wasko, Kamil Rozniakowski, Krystian Galecki, Zbigniew J. Kaminski, Beata Kolesinska
Summary: The study identified a cyclic fragment (1-7) H-KCNTATC-OH with a disulfide bond as an inhibitor capable of inhibiting the aggregation of amyloidogenic cores of the hormone related to diabetes mellitus. Additionally, this peptide was found to inhibit insulin hot spot aggregation, indicating its universal potential in inhibiting the aggregation process of hormones regulating carbohydrate metabolism. Ongoing research is focusing on the extensive use of this cyclic fragment as a peptide inhibitor in the polypeptide/protein aggregation process.
CHEMISTRY & BIODIVERSITY
(2021)
Article
Materials Science, Multidisciplinary
Katarzyna Mitura, Joanna Kornacka, Elzbieta Kopczynska, Jacek Kalisz, Ewa Czerwinska, Maciej Affeltowicz, Witold Kaczorowski, Beata Kolesinska, Justyna Fraczyk, Totka Bakalova, Lucie Svobodova, Petr Louda
Summary: Carbon-based nanomaterials are widely used in biomedical applications and play an important role in bactericidal activity against food pathogens. Research has shown that the properties and bactericidal effects of carbon nanomaterials are influenced by factors such as grain size and surface modification.
Article
Biochemistry & Molecular Biology
Lukasz Janczewski, Dorota Kregiel, Beata Kolesinska
Summary: Thirty-three alkyl and aryl isothiocyanates, as well as their derivatives, were successfully synthesized with satisfactory yields using a one-pot, two-step procedure in the presence of organic base and carbon disulfide. The antibacterial activity of these compounds was evaluated, with ITC 9e showing the highest activity against E. coli and S. aureus bacterial strains.
Article
Biochemistry & Molecular Biology
Dawid Maliszewski, Agnieszka Wrobel, Beata Kolesinska, Justyna Fraczyk, Danuta Drozdowska
Summary: A series of new analogs of nitrogen mustards containing the 1,3,5-triazine ring substituted with dipeptide residue were synthesized and evaluated for the inhibition of both acetylcholinesterase (AChE) and beta-secretase (BACE1) enzymes. The most active compounds against both AChE and BACE1 were identified as compounds A and 4a, with considerable inhibitory concentrations.
Article
Pharmacology & Pharmacy
Malgorzata Matusiak, Beata P. Rurarz, Slawomir Kadlubowski, Marian Wolszczak, Urszula Karczmarczyk, Michal Maurin, Beata Kolesinska, Piotr Ulanski
Summary: Radiation crosslinking was used to create nanocarriers based on poly(acrylic acid) for targeted delivery of radioactive isotopes. The internally crosslinked hydrophilic macromolecules-nanogels were conjugated with bombesin-derivative-oligopeptide and tetraazacyclododecane-1,4,7,10-tetraacetate for selective radioligand transport. The efficiency of radiolabeling nanocarriers with Lu-177 was chromatographically tested, showing promise for theranostic applications.
Article
Biochemistry & Molecular Biology
Lukasz Banach, Lukasz Janczewski, Jakub Kajdanek, Katarzyna Milowska, Joanna Kolodziejczyk-Czepas, Grzegorz Galita, Wioletta Rozpedek-Kaminska, Ewa Kucharska, Ireneusz Majsterek, Beata Kolesinska
Summary: Eight dipeptides containing antifibrinolytic agents were successfully synthesized using a three-step process. Hemolysis tests and clotting tests showed that these compounds had no significant effects on blood components and clotting time. The clot formation and fibrinolysis assay revealed that two of the compounds exhibited high activity in the plasma coagulation cascade and fibrinolytic efficiency. The cytotoxicity and genotoxicity evaluation demonstrated that the synthesized dipeptides caused minimal damage to cells and DNA.
Article
Biochemistry & Molecular Biology
Jolanta Kryczka, Jakub Kryczka, Lukasz Janczewski, Anna Gajda, Andrzej Fraczyk, Joanna Boncela, Beata Kolesinska, Ewa Brzezianska-Lasota
Summary: One of the main challenges in the treatment of non-small-cell lung cancer is the development of resistance to cisplatin-based chemotherapy. This study investigates the potential of isothiocyanates (ITCs) and the ALDH inhibitor disulfiram (DSF) as adjuvants to cisplatin chemotherapy. The results show that certain ITCs and DSF can reduce the cisplatin tolerance of resistant lung cancer cells, suggesting their potential in overcoming drug resistance.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Marcin Kolasa, Katarzyna Czerczak, Justyna Fraczyk, Lukasz Szymanski, Slawomir Lewicki, Anna Bednarowicz, Nina Tarzynska, Dominik Sikorski, Grzegorz Szparaga, Zbigniew Draczynski, Szczepan Cierniak, Urszula Brzoskowska, Grzegorz Galita, Ireneusz Majsterek, Dorota Bociaga, Paulina Krol, Beata Kolesinska
Summary: New scaffold materials composed of biodegradable components are of great interest in regenerative medicine. In this study, peptide-polysaccharide conjugates were analyzed as scaffolds for regenerative medicine. The conjugates containing calcium alginate and the ternary nonwoven material were found to be the most supportive of muscle tissue regeneration. Peptides 1 and 2 exhibited the best biological properties and showed high antibacterial activity against Staphylococcus aureus and Klebsiella pneumoniae. These findings suggest that polysaccharide conjugates with peptides 1 and 2 have potential applications in regenerative medicine.
Article
Biochemistry & Molecular Biology
Dominik Sikorski, Piotr Rosiak, Lukasz Janczewski, Marek J. Potrzebowski, Dorota Kregiel, Slawomir Kazmierski, Damian Neubauer, Beata Kolesinska, Justyna Fraczyk, Anna Adamczyk, Zbigniew Draczynski
Summary: This work investigates the use of chitosan films containing ciprofloxacin on their surface. The results show that ciprofloxacin undergoes a crystalline-to-amorphous transition when applied on the chitosan film, leading to improved bioavailability and antimicrobial efficiency. Furthermore, the study confirms the correlation between the concentration of ciprofloxacin and the antibacterial activity of the chitosan films.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Kamila Baran, Joanna Wasko, Jakub Kryczka, Joanna Boncela, Slawomir Jablonski, Beata Kolesinska, Ewa Brzezianska-Lasota, Jacek Kordiak
Summary: A comparative analysis using high-performance liquid chromatography coupled to mass spectrometry was conducted to identify key proteins in serum exosomes that play a crucial role in the development and metastasis of non-small lung cancer. The study identified 150 NSCLC proteins involved in osmoregulation, cell adhesion, cell motility, and differentiation, with three proteins showing significant involvement in cancer-associated fibroblasts and tumor-associated macrophages infiltration processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Krystian Galecki, Agnieszka Kowalska-Baron, Katarzyna E. Nowak, Anna Gajda, Beata Kolesinska
Summary: This study demonstrates the utility of time-resolved fluorescence spectroscopy in detecting subtle changes in the microenvironment of tryptophan chromophore in AOT reverse micelles. The results show evident changes in spectral characteristics and fluorescence decay kinetics when tryptophan and its peptides are inserted into micelles.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Angelika Becht, Justyna Fraczyk, Joanna Wasko, Elzbieta Menaszek, Jakub Kajdanek, Katarzyna Milowska, Beata Kolesinska
Summary: The aim of this research was to select and assess the potential usefulness of the fragments that make up the outer layer of the collagen IV (COL4A6) protein for regenerative medicine. Cellulose-immobilized protein fragment libraries treated with polyclonal anti-collagen IV antibodies were used to select the peptides forming the outer sphere of collagen IV. The selected fragments were found to be non-toxic and further research is ongoing on their potential uses in regenerative medicine.
JOURNAL OF PEPTIDE SCIENCE
(2023)
Article
Engineering, Biomedical
Anna M. Tryba, Malgorzata Krok-Borkowicz, Michal Kula, Natalia Piergies, Mateusz Marzec, Erik Wegener, Justyna Fraczyk, Rainer Jordan, Beata Kolesinska, Dieter Scharnweber, Czeslawa Paluszkiewicz, Elzbieta Pamula
Summary: A degradable PLGA membrane modified with cell adhesive RGD motifs was designed for the treatment of bone tissue defects. The modified PLGA membrane showed improved bone cell adhesion, proliferation, and viability compared to non-modified PLGA membranes.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2022)
Article
Chemistry, Multidisciplinary
Lukasz Janczewski, Dariusz Zielinski, Beata Kolesinska
Summary: In this work, a novel method for the synthesis of ester and amide derivatives containing furan rings under mild synthetic conditions using microwave radiation was presented. The reactions were optimized for parameters such as reaction time, solvent, and substrate amounts, resulting in good yields after isolation. The method also allowed for the synthesis of compounds with monoamide, ester, diamides, and diester bonds.