4.7 Article

Rapid color-fading colorimetric sensing of Hg in environmental samples: regulation mechanism from DNA dimension

Journal

MICROCHIMICA ACTA
Volume 189, Issue 2, Pages -

Publisher

SPRINGER WIEN
DOI: 10.1007/s00604-022-05177-w

Keywords

Color-fading colorimetric sensor; Mercury detection; DNA dimension; Real environmental media; Sensing mechanism

Funding

  1. National Natural Science Foundation of China [21605018]
  2. Natural Science Basic Research Project of Shaanxi Province of China [2020JM-528, 2021JZ-52]

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The dimension change of aptamer DNA was found to significantly weaken the mimicking activity of gold nanozyme, contrary to previous research. Based on this, a rapid colorimetric method for detecting low concentrations of mercury in environmental media was developed. The method was fast, sensitive, and reliable in practical application.
It was found that dimension change of aptamer DNA significantly weakened the mimicking activity of gold nanozyme, which was contrary to previous research. Based on this, a rapid colorimetric method for the detection of low concentrations of mercury in environmental media was fabricated. It was observed that 40 nM Hg2+ causes color changes in solution. The detection limit of absorbance measurements was estimated to be 9.3 x 10(-11) M. The assay was fast and could complete a single test in half an hour. The detection results for real environment samples confirmed the reliability of the colorimetric analysis in practical application. The proposed assay provides an alternative method for real-time monitoring of mercury in the environment. In particular, the charge effect on the affinity of nanozyme consummated the DNA regulation mechanism for the simulated enzyme activity.

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