Article
Food Science & Technology
Hanaa M. M. Elhaddad, Hala M. Hammoda, Doaa A. Ghareeb, Fatma A. A. Mahmoud, Ahmed Hussein, Mokhtar Ibrahim Yousef, Reham S. Darwish, Eman Shawky
Summary: This study aimed to investigate the chemistry and anti-inflammatory activity of different fractions of olibanum species extracted by various procedures. Chemical profiling of the fractions was conducted using UPLC/MS/MS coupled with chemometric analysis. The anti-inflammatory activity of the extracts was evaluated on LPS-stimulated WBCs, and the efficacy-directed markers were identified using multivariate analysis. The results revealed that the composition of olibanum constituents was influenced more by the geographical source of the resin rather than the extraction procedure, and different fractions showed strong correlations with the inhibition of specific pro-inflammatory markers.
Article
Pharmacology & Pharmacy
Yin Yuan, Fei-Xue Dong, Xu Liu, Hong-Bin Xiao, Zhong-Guang Zhou
Summary: This study revealed that Magnoflorine has an intervention effect on prostatitis, inhibiting its development by regulating metabolic pathways and biomarkers. The results suggest that alkaloids could be an effective therapy for prostatitis, with specific metabolic pathways closely related to the condition identified.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Medicine, Research & Experimental
Huan Li, Liang Wang, Jing Zhu, Jialing Xiao, Huining Yang, Huanyue Hai, Jiarui Hu, Lin Li, Yi Shi, Man Yu, Ping Shuai, Yuping Liu, Xueming Ju, Gang Wu, Yu Zhou, Bolin Deng, Bo Gong
Summary: The study aimed to find reliable serum biomarkers for diagnosing ankylosing spondylitis (AS). Serum samples from 20 AS patients and 20 healthy controls (HCs) were analyzed using UPLC-MS/MS. Differential metabolites between AS patients and HCs were identified, and pathway analysis and heat map were conducted. Predictive and diagnostic models were established using random forest (RF) and LASSO regression. A total of 61 differential metabolites were identified, and RF model showed 95% predictive accuracy and a high AUC. A diagnostic model consisting of nine metabolites was generated, capable of distinguishing HCs from AS with a high AUC. UPLC-MS/MS analysis proved to be a powerful tool for identifying AS metabolite profiles, and the identified biomarkers had diagnostic value for AS.
CLINICAL AND EXPERIMENTAL MEDICINE
(2023)
Article
Medical Laboratory Technology
Zheng Zhong, Yukai Huang, Qidang Huang, Shaoling Zheng, Zhixiang Huang, Weiming Deng, Tianwang Li
Summary: This study investigated the serum metabolic profiling of gout patients compared to healthy controls using UPLC-Q-TOF/MS, revealing significant differences in metabolites between the two groups. Bilirubin was identified as a potential biomarker for gout, and primary bile acid biosynthesis was suggested as a novel metabolic pathway.
CLINICA CHIMICA ACTA
(2021)
Article
Food Science & Technology
Dina A. Selim, Eman Shawky, Doaa A. Ghareeb, Shaymaa A. Abdulmalek, Rasha M. Abu El-Khair
Summary: This study aimed to analyze the chemical constituents and anti-inflammatory activity of two widely distributed Salsola species using UHPLC-QqQ-MS. The results showed that S. imbricata contained phytosterols, while S. jordanicola contained phenylalkylamines. Additionally, S. imbracta reduced TNF-alpha levels, and the petroleum ether and n-butanol fraction of S. jordanicola were more effective than piroxicam in reducing IL-6 gene expression.
Article
Biochemistry & Molecular Biology
Fengfeng Xie, Liba Xu, Hua Zhu, Yaling Chen, Yinlan Li, Lizhen Nong, Yanfang Zeng, Sijie Cen
Summary: This study found that ellagic acid can regulate immune response, inhibit inflammatory reaction, and reduce body temperature, thus demonstrating its antipyretic and anti-inflammatory effects.
Article
Rheumatology
Yiyi Gong, Panpan Zhang, Zheng Liu, Jieqiong Li, Hui Lu, Yujie Wang, Bintao Qiu, Mu Wang, Yunyun Fei, Hua Chen, Linyi Peng, Jing Li, Jiaxin Zhou, Qun Shi, Xuan Zhang, Min Shen, Xiaofeng Zeng, Fengchun Zhang, Wen Zhang
Summary: Metabolomic profiling of IgG4-RD patients revealed significant alterations in plasma metabolites compared to healthy controls and post-treated patients. Certain metabolites, such as UTP, UDP-Glc/UDP-Gal, and LysoPC (18:1), showed high sensitivity and specificity in diagnosing IgG4-RD. The study suggested that metabolomic profiling could offer valuable insights into the pathophysiology of IgG4-RD and serve as potential biomarkers for diagnosis.
Article
Plant Sciences
Huanhuan Wang, Liying Tang, Shaowei Hu, Xixian Kong, Yi Ouyang, Dong Zhang, Yi Zhang, Shihuan Tang, Hongwei Wu, Hongjun Yang
Summary: This study revealed the ingredients, tissue distribution, and pharmacokinetic characteristics of Shengmai injection (SMI). The study found that ginsenosides were the main active compounds in SMI and had high exposure in different tissues. The pharmacokinetic evaluation showed different elimination rates for different types of ginsenosides.
JOURNAL OF ETHNOPHARMACOLOGY
(2024)
Article
Food Science & Technology
Xinxin Cao, Mengmeng Liu, Yajie Hu, Qiang Xue, Fan Yao, Jing Sun, Liwei Sun, Yujun Liu
Summary: This study revealed that ripe Pu-erh tea and raw Pu-erh tea have similar but lower chemical components and antioxidant activities, and metabolites play a significant role in distinguishing different types of teas and fermentation processes.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Agricultural Engineering
Safa M. Shams Eldin, Eman Shawky, Shaimaa M. Sallam, Nefertiti El-Nikhely, Samah M. El Sohafy
Summary: This study utilized a metabolomic approach to investigate the chemical constituents in the roots and herbs of three Echinacea species and identified immunomodulatory biomarkers. The roots of E. angustifolia were separate from the other samples due to specific secondary metabolites. While the roots of Echinacea species exhibited similar immunomodulatory responses, the herbs were less efficient as immunomodulators.
INDUSTRIAL CROPS AND PRODUCTS
(2021)
Article
Plant Sciences
Fan He, Yu-Feng Huang, Wei Dai, Xian-You Qu, Jing-Guang Lu, Chi-Chou Lao, Wen-Hui Luo, Dong-Mei Sun, Mei Wei, Sheng-Yuan Xiao, Ying Xie, Liang Liu, Hua Zhou
Summary: In this study, the TOF-SIMS image analysis technology was used for the first time to detect Chinese herbal medicine. Taking Coptis rhizome as an example, the distribution of nine active compounds in the cross-section of Coptis rhizome was studied. The results showed that these compounds had different distribution patterns in the cross-section and their contents increased with the growth years.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Linlin Liu, Xueying Han, Guoshun Shan, Li Fu, Deqiang Dou
Summary: This study investigated the different mechanisms between different natures of ginseng medicines and their qi-tonifying action, as well as the nature of black ginseng. The results showed that ginseng medicines could treat hyperthyroidism by regulating the hypothalamus pituitary thyroid (HPT) axis, with black ginseng exhibiting a mild-warm nature. The study also revealed the involvement of HPA and HPG axes in the qi-tonifying effects of ginseng medicines.
JOURNAL OF ETHNOPHARMACOLOGY
(2024)
Article
Chemistry, Applied
Xi Juan Zhao, Peng Mei Guo, Wen Hui Pang, Tao Tan, Yao Hai Zhang, Bi Ning Jiao
Summary: This study investigated the secondary metabolites of Jindou kumquat and identified the dominant characteristic metabolites. The findings provide a basis for further evaluation and utilization of Fortunella germplasms.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Plant Sciences
Shiqin Jiang, Xiuting Shen, Shenxin Xuan, Bao Yang, Qingfeng Ruan, Hui Cui, Zhongxiang Zhao, Jing Jin
Summary: Croton crassifolius Geisel (CCG) extract has shown therapeutic potential against ulcerative colitis (UC) by modulating metabolic pathways and reducing inflammation, offering a new strategy for UC management.
Article
Chemistry, Analytical
Yu Yang, Zhe Chen, Guangli Yan, Ling Kong, Le Yang, Hui Sun, Ying Han, Jie Zhang, Xijun Wang
Summary: This study evaluated the sedative and hypnotic effects of Naoling Pian (NLP) in treating insomnia and found that it can improve neurotransmitter levels related to sleep in rat brain tissue. Metabolomics analysis also revealed the regulatory effects of NLP on potential biomarkers of insomnia.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Analytical
Mohammed Hamed Alqarni, Mohamad Ayman Salkini, Prawez Alam, Mazen Talal Alanazi, Maged Saad Abdel-Kader, Samah M. El Sohafy
Summary: Plants' secondary metabolites undergo qualitative and quantitative variations due to environmental and growth factors. Selecting the appropriate time for collection of medicinal plants is crucial to obtain the maximum content of active components and ensure maximum efficacy. The study revealed considerable variations in the level of oleuropein, the main active component of Olive leaves, throughout the year. Two points of high levels of oleuropein were detected prior to flowering stage and fruit ripening.
ACTA CHROMATOGRAPHICA
(2022)
Article
Biochemical Research Methods
Hend M. Dawood, Eman Shawky, Hala M. Hammoda, Aly M. Metwally, Reham S. Ibrahim
Summary: Aromatase is an enzyme that converts androgens into oestrogens, and inhibiting its activity is a target in post-menopausal breast cancer therapy. The TLC-bioautography technique was employed to evaluate aromatase inhibitory activity for the first time. The study aimed to develop a new method for determining aromatase inhibitory activity in plant extracts and compared two quantitation methods to attain reliable results.
PHYTOCHEMICAL ANALYSIS
(2022)
Article
Agricultural Engineering
Reham S. Darwish, Marina A. A. Fares, Hala M. A. Hammoda, Abdelrahman M. Yassin, Doaa A. Ghareeb, Fathallah M. Harraz, Eman Shawky
Summary: Mesquite (Prosopis juliflora) is utilized in various industries for its medicinal purposes. Chemical profiling of different parts of mesquite showed distinct variations in their chemical compositions and anti-inflammatory activities. Stems extracts exhibited significant inhibition of pro-inflammatory markers, suggesting the potential use of mesquite as a source of functional components.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Chemistry, Applied
Dina A. Selim, Eman Shawky, Rasha M. Abu El-Khair
Summary: In this study, the metabolic profiling of seven Sri Lankan tea grades was conducted using UPLC-MS/MS combined with chemometrics. Different chemical markers were identified for different types and grades of tea, and variations in metabolites were observed between different grades.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Chemistry, Applied
Reham S. Darwish, Hala M. Hammoda, Doaa A. Ghareeb, Ali S. A. Abdelhamid, Fathallah M. Harraz, Eman Shawky
Summary: The study identified several compounds in Platycladus orientalis leaves with anti-inflammatory properties, such as afzelin, myricetin, apigenin-7-O-hexoside, quercetrin, and hyperoside. Main inflammation-related targets included IL2, VEGFA, AKT1, AKT2, CREB1, IL5, RPS6KB1, and TNF. Quercetrin and hyperoside demonstrated anti-inflammatory activity, indicating strong synergistic interactions with inflammation and immunity-related targets and pathways.
NATURAL PRODUCT RESEARCH
(2022)
Article
Chemistry, Multidisciplinary
Dina S. Ghallab, Eman Shawky, Ali M. Metwally, Ismail Celik, Reham S. Ibrahim, Mohamed M. Mohyeldin
Summary: In this study, novel pharmacologically active chemical entities were discovered from Egyptian propolis using computer-aided molecular docking and MD simulations. These compounds showed inhibitory effects on xanthine oxidase (XO) and exhibited synergistic effects when combined with allopurinol or febuxostat. This research is important for developing new therapeutic options for hyperuricemia.
Article
Food Science & Technology
Yasmin A. Mahgoub, Eman Shawky, Moustafa Eldakak, Mohammed Bahey-El-Din, Fikria A. Darwish, Nadia A. El Sebakhy, Amr El-Hawiet
Summary: This study successfully authenticated German Chamomile using DNA barcoding and chemical profiling, and discriminated it from related toxic adulterants. The combination of genetic and metabolomic fingerprinting proved to be an effective tool in authenticating German Chamomile and distinguishing it from its adulterants, providing a comprehensive approach for quality control in the food industry.
Article
Biochemistry & Molecular Biology
Yaohan Li, Shengzhi Liu, Eman Shawky, Minglei Tao, Amin Liu, Kaisa Sulaiman, Jingkui Tian, Wei Zhu
Summary: This study revealed the response mechanism of Morus alba leaves to UV-B radiation and dark incubation through quantitative proteomic analysis. Proteins related to photosynthesis, amino acid biosynthesis, and tocopherol biosynthesis were significantly altered in the UVB group, while proteins related to the biosynthesis of phenolic compounds were significantly altered in the UVD group. Additionally, the abundances of proteins involved in the ubiquitin-proteasome system (UPS) were significantly increased in both UVB and UVD groups, suggesting that UPS and secondary defense mechanism participate in the resistance to UV-B radiation and dark incubation.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2022)
Article
Agriculture, Multidisciplinary
Nihal M. El Newehy, Mohammad M. Abd-Alhaseeb, Gamal A. Omran, Fathallah M. Harraz, Eman Shawky
Summary: This study utilized metabolomics analysis to investigate different parts of pomegranate fruit, revealing their potential as sources of bioactive compounds. The findings highlight the importance of utilizing the full resources of pomegranate fruit and its waste.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2022)
Article
Agricultural Engineering
Seif A. Abouheif, Shaimaa M. Sallam, Samah M. El Sohafy, Fahima F. Kassem, Eman Shawky
Summary: This study found that the use of natural deep eutectic solvents (NADES) significantly improves the bioavailability of curcuminoids, with choline chloride: propylene glycol (DES 3) being the most efficient extract. The bioavailability of curcuminoids extracted using DES 3 was 450.4% higher than ethanol extracts, indicating a significant increase in absorption.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Plant Sciences
Asmaa Mahana, Hala M. Hammoda, Asmaa A. Khalifa, Samar S. Elblehi, Fathallah M. Harraz, Eman Shawky
Summary: This study explored the effects of different organs of the Physalis pruinosa plant on diabetes in rat models and identified the potential bioactive compounds. The results showed that the leaves of P. pruinosa had significant hypoglycemic and insulin-secreting effects. This research provides theoretical support for the treatment of diabetes.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Food Science & Technology
Dina S. Ghallab, Eman Shawky, Doaa A. Ghareeb, Shaymaa A. Abdulmalek, Soad M. Mohy El-Din, Mohamed M. Mohyeldin, Reham S. Ibrahim
Summary: This study provides a comprehensive strategy to identify potential anti-inflammatory biomarkers in marine algae using bioinformatics, bio-guided fractionation, and metabolomics. The results suggest that marine algae have multi-targeted synergistic functions and can effectively mitigate inflammation.
Article
Food Science & Technology
Dina A. Selim, Eman Shawky, Doaa A. Ghareeb, Shaymaa A. Abdulmalek, Rasha M. Abu El-Khair
Summary: This study aimed to analyze the chemical constituents and anti-inflammatory activity of two widely distributed Salsola species using UHPLC-QqQ-MS. The results showed that S. imbricata contained phytosterols, while S. jordanicola contained phenylalkylamines. Additionally, S. imbracta reduced TNF-alpha levels, and the petroleum ether and n-butanol fraction of S. jordanicola were more effective than piroxicam in reducing IL-6 gene expression.
Article
Chemistry, Analytical
Yasmin A. Mahgoub, Eman Shawky, Doaa A. Ghareeb, Fikria A. Darwish, Nadia A. El Sebakhy, Amr M. El-Hawiet
Summary: This study investigates the accumulation dynamics of different components of silymarin in different organs of purple and white flowers of Silybum marianum at different developmental stages, and finds that the fruit extract of purple flowers genotype has the most prominent anti-inflammatory activity. Furthermore, specific silymarin components are found in the flowers and leaves at the post-fruiting early drying stage.
MICROCHEMICAL JOURNAL
(2023)
Article
Engineering, Electrical & Electronic
Eman Shawky, Moustafa H. H. Aly, Mohamed El-Shimy
Summary: In this paper, we discuss an underwater optical wireless communication system that utilizes visible light communication for its wide spectrum, high data rate, and high accuracy. The novelty lies in improving the channel estimation between transmitter and receiver using Kalman Filter (KF), which shows better performance than traditional methods. The scenario involves transmitting data through underwater harbor and coastal channels, with two channel models and two modulation techniques. The simulation results demonstrate that ACO-OFDM with CEAPF and KF achieves the lowest bit error rate compared to other channel estimation methods.
OPTICAL AND QUANTUM ELECTRONICS
(2023)
