Article
Agriculture, Dairy & Animal Science
Zheng Pan, Aiqian Ye, Anant Dave, Karl Fraser, Harjinder Singh
Summary: This study investigated the effect of UHT treatment and pH values on the physical properties of sheep skim milk. It was found that significant sedimentation occurred in UHT-treated milk at natural pH and pH 7.0, while lower sedimentation was observed at pH values of 6.7 to 6.9. Additionally, UHT treatment resulted in decreased pH and ionic calcium level of the milk.
JOURNAL OF DAIRY SCIENCE
(2023)
Article
Food Science & Technology
Simon Schiffer, Eva Scheidler, Tim Kiefer, Ulrich Kulozik
Summary: The study found that adding low amounts of calcium at low temperatures can reduce the casein content in milk serum, while adjusting pH separately has a minor effect on casein concentration and composition in the serum.
Article
Agriculture, Dairy & Animal Science
Zheng Pan, Aiqian Ye, Anant Dave, Karl Fraser, Harjinder Singh
Summary: This study characterized the interactions among proteins in sheep skim milk during heat treatments and their effects on the size and structure of casein micelles. The results showed that the denaturation and association of whey proteins with casein micelles increased with increasing heating temperature and time. The size of casein micelles also increased depending on the heating conditions and the level of association with denatured whey proteins. The aggregation of casein micelles and the association of whey proteins with micelles occurred simultaneously during heating.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Food Science & Technology
Dominik Guggisberg, Sonja Loosli, Carola Blaser, Rene Badertscher, Remo Schmidt
Summary: This study evaluated the impact of storage conditions (time, 0-48 h; temperature, 4-16 ?) of raw cheese milk on coagulation properties using diffusion wave spectroscopy. The results showed that storage time and temperature had significant effects on rennet coagulation time (RCT) and curd firmness. Different temperature conditions resulted in different changes in RCT and curd firmness with varying storage times.
INTERNATIONAL DAIRY JOURNAL
(2022)
Article
Chemistry, Applied
N. Callaghan-Patrachar, F. Peyronel, D. A. Pink, A. G. Marangoni, C. P. Adams
Summary: The study using neutron scattering techniques revealed the fractal structures with properties similar to swollen, randomly-branched polymers induced by rennet in commercial milk. Calf rennet resulted in a larger fractal dimension in skim milk, while fungal rennet resulted in smaller structural units in milk with a slightly larger fractal dimension of 2.5. These measurements serve as an important foundation for further structural studies of commercial cheese using scattering techniques.
FOOD HYDROCOLLOIDS
(2021)
Article
Agriculture, Dairy & Animal Science
Dimuthu Bogahawaththa, Biljana Trajkovska, Tatijana Markoska, Todor Vasiljevic
Summary: Heating, pressurization, and shearing can modify native milk proteins, affecting their stability and dissociation. Pressure treatment and evaporative concentration can lead to dissociation and aggregation of casein micelles under different temperatures.
JOURNAL OF DAIRY SCIENCE
(2021)
Article
Agriculture, Multidisciplinary
Mathias Jaeser, Ulrike Moeckel, Kati Weigel, Thomas Henle
Summary: The lysozyme content in the milk of volunteering mothers was quantified using RP-HPLC-UV-ESI-Q-TOF, and it was found to be mainly bound to casein micelles. The enzymatic activity of lysozyme was similar for both micelle-bound and free protein, indicating that the micellar structure does not affect its antibacterial activity. These results suggest that lysozyme is an integral component of casein micelles in human milk.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Applied
Jielong Zhang, Dasong Liu, Xiumei Tao, Jun Tang, Xiaoyu Peng, Thom Huppertz, Xiaoming Liu, Peng Zhou
Summary: The effect of enzymatic dephosphorylation on the structure of casein micelles in caprine micellar casein concentrate was studied. The results showed that with increasing dephosphorylation, the molar mass decreased, the radii decreased, the hydration increased, and the internal protein inhomogeneity disappeared.
FOOD HYDROCOLLOIDS
(2024)
Article
Engineering, Chemical
Arissara Phosanam, Jayani Chandrapala, Thom Huppertz, Benu Adhikari, Bogdan Zisu
Summary: The physicochemical characteristics of whey protein and casein powders were studied under different temperature and humidity conditions during storage. It was found that the moisture content, glass transition temperature, and browning were all dependent on temperature and humidity.
Article
Agriculture, Dairy & Animal Science
S. Touhami, A. Marciniak, A. Doyen, G. Brisson
Summary: Mechanical and physicochemical treatments of milk can modify the structure of casein micelles and impact their techno-functional properties. Alkalinization and ultra-high-pressure homogenization have the potential to improve the functionality of dairy ingredients.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Chun-Chi Chen, Liang-Yu Chen, Wen-Tai Li, Ken-Lin Chang, Meng- Kuo, Chao-Jung Chen, Jung-Feng Hsieh
Summary: The study investigated the effects of chymosin on the physicochemical and hydrolysis characteristics of casein micelles and individual caseins. Chymosin led to coagulation and decreased particle size of the casein micelles, as well as hydrolyzed beta-CN, kappa-CN, and alpha(s)-CN but not beta-LG. Additionally, it was found that chymosin hydrolyzed kappa-CN before beta-CN and alpha(s)-CN.
Article
Chemistry, Physical
Julien Bauland, Mathieu Leocmach, Marie-Helene Famelart, Thomas Croguennec
Summary: One of the first steps in cheese making is to destabilize casein micelles and initiate milk gelation through enzymatic hydrolysis. The enzymatic milk gel is then cut to promote syneresis and expulsion of the soluble phase. This study investigates the non-linear properties and yielding behavior of enzymatic milk gels through creep, fatigue, and stress sweep tests. The results highlight the importance of the nanoscale organization of casein micelles for the macroscopic mechanical properties of the gel.
Article
Food Science & Technology
Giuseppe Natrella, Aristide Maggiolino, Pasquale De Palo, Marina Mefleh, Michele Faccia
Summary: The effect of ultrafiltration on the cheesemaking properties of donkey milk was assessed. The main limiting factor for coagulation was found to be the milk protein concentration, and the addition of EPS-producing starter yielded the best results.
INTERNATIONAL DAIRY JOURNAL
(2024)
Article
Chemistry, Applied
Julien Bauland, Marie-Helene Famelart, Marc Faiveley, Thomas Croguennec
Summary: Casein micelles are colloids composed of milk proteins and minerals. The composition and properties of casein micelles can vary with changes in the physicochemical conditions of milk. This study investigates the effect of cation partitioning in casein micelles on the rheology of enzymatic milk gel. The results show that the rheological properties of the gel are closely correlated with the types and content of cations in casein micelles.
FOOD HYDROCOLLOIDS
(2022)
Article
Chemistry, Applied
Davor Daniloski, Noel A. McCarthy, Tatijana Markoska, Martin J. Auldist, Todor Vasiljevic
Summary: This study highlights differences in conformational and physicochemical characteristics of bovine skim milk and micellar casein from cows of different beta-casein phenotypes, with FTIR and NMR spectroscopies used for analysis. Results showed significant variations in the secondary structure of milk proteins among genetic variants, which were slightly affected by temperature.
FOOD HYDROCOLLOIDS
(2022)
Article
Chemistry, Applied
Shuangshuang Wang, Zhaojun Liu, Yuxuan Song, Yan Zhang, Lili Zhao, Lei Zhang, Xin Lue, Haiyan Wang, Ximei Zhang, Jiaying Zhang, Wupeng Ge
Summary: This study characterized the lipid compositions of human and ewe colostra using a quantitative lipidomics approach. It identified 173 significantly different lipids and selected four potential lipid biomarkers. These different lipids were involved in 44 metabolic pathways.
Article
Clinical Neurology
Yating Jian, Heying Wang, Lili Zhao, Tao Li, Lei Zhang, Xiaoya Wang, Yiheng Zhang, Ye Li, Meijuan Dang, Ziwei Lu, Jialiang Lu, Yuxuan Feng, Yang Yang, Guilian Zhang
Summary: This study aims to evaluate the impact of metformin treatment on the prognosis of patients with acute ischemic stroke (AIS) and found that patients who received continuous metformin treatment before stroke onset and after admission had improved functional outcomes at 90 days.
NEUROLOGICAL SCIENCES
(2023)
Article
Neurosciences
Tao Li, Lili Zhao, Hong Fan, Zhiyang Chen, Ye Li, Meijuan Dang, Ziwei Lu, Jialiang Lu, Qiao Huang, Heying Wang, Shengxi Wu, Guilian Zhang, Fang Kuang
Summary: This study investigated the neuroinflammatory response in rats following injection of ioversol during angiography. The results showed that ioversol induced neuroinflammation and oxidative stress in the brain, resulting in cell toxicity and activation of microglia. Furthermore, the study demonstrated that administration of an antioxidant drug could effectively inhibit the neuroinflammatory response caused by ioversol.
NEUROTOXICITY RESEARCH
(2023)
Article
Cell Biology
Xue-Ming Zhu, Lin Li, Jian-Dong Bao, Jiao-Yu Wang, Shuang Liang, Li-Li Zhao, Chang-Li Huang, Jiong-Yi Yan, Ying-Ying Cai, Xi-Yu Wu, Bo Dong, Xiao-Hong Liu, Daniel J. Klionsky, Fu-Cheng Lin
Summary: This study identified a new VASt domain-containing protein, MoVast2, and uncovered its regulatory mechanism in M. oryzae. MoVast2 interacted with MoVast1 and MoAtg8, and colocalized at the PAS, leading to inappropriate autophagy progress in the Delta Movast2 mutant. Additionally, MoVast2 maintained lipid homeostasis and autophagy balance by regulating TOR activity in M. oryzae.
Correction
Gastroenterology & Hepatology
Ling Mei, Ying Ma, Lili Zhao, Qingling Chen, Li Zhou, Hang Yang, Jie Liu, Jia Li
BMC GASTROENTEROLOGY
(2023)
Article
Engineering, Environmental
Xiaoting Zhang, Lili Zhao, Yu Qiu, Yan Wang, Yueyao Fan, Xueru Liu, Bin Cui
Summary: In this study, a core-shell structure was designed by coating SrZrO3-BiMg0.5Sn0.5O3 (SZ-BMS) on the surface of Na0.4K0.1Bi0.5TiO3 (NKBT) and then coating SiO2 glass to prepare NKBT@(SZ-BMS)@SiO2 ceramics. The SZ-BMS phase played multiple roles in alleviating interfacial polarization, domain reconstruction, and defect regulation, while the outer SiO2 layer benefited grain size refinement and defect control. The ceramics achieved high breakdown strength of 301 kV/cm and polarization of 29.1 mu C/cm² when the BMS amount was 15 mol%. Ultimately, large recoverable energy density of 3.94 J/cm³ with high efficiency of 87.1% were obtained, along with ultra-wide temperature stability and excellent thermal endurance.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Pharmacology & Pharmacy
Xihua Yang, Shuaina Zhang, Jiao He, Lili Zhao, Lixia Chen, Yongming Yang, Jing Wang, Lei Yan, Tingting Zhang
Summary: This study investigated the mechanisms of brazilin-induced cell death in T24 bladder cancer cells and found that brazilin induced necrosis and upregulated the expression of necroptosis-related genes and proteins. Brazilin also repressed caspase 8 expression and decreased mitochondrial membrane potentials. These findings suggest that brazilin induces cell death in bladder cancer cells through necroptosis and may be explored as an anticancer agent.
CLINICAL AND EXPERIMENTAL PHARMACOLOGY AND PHYSIOLOGY
(2023)
Article
Microbiology
Yiling Zuo, Chao He, Dongdong Zhang, Lili Zhao, Xueli He, Xiang Sun
Summary: In this study, the changes of soil microbial community structure and function in an extremely arid desert were determined by examining the microbial composition and catabolic metabolism. The results showed that regardless of plant species and seasonal patterns, the microbial community was mostly dominated by gram-negative bacteria, and the main carbon sources for desert microbial metabolism were carbohydrates, amino acids, and polymers. The study also found that soil factors driven by desert shrubs and climate factors were the main influences on the seasonal patterns of soil microbial community and metabolic function in the desert.
MICROBIOLOGICAL RESEARCH
(2023)
Article
Neurosciences
Yunsong Zhang, Yinying Shen, Li Zhao, Qian Zhao, Lili Zhao, Sheng Yi
Summary: Nerve injury-induced Schwann cell dedifferentiation promotes axon growth by creating a favorable microenvironment. Transcription factor BCL11A plays a crucial role in Schwann cell reprogramming during peripheral nerve regeneration. Silencing Bcl11a reduces Schwann cell viability, proliferation, migration rates, and debris clearance ability. Bcl11a deficiency leads to restricted axon elongation and myelin wrapping, resulting in failed recovery. Mechanistically, BCL11A regulates Schwann cell activity by binding to the Nr2f2 promoter and modulating Nr2f2 expression. In conclusion, BCL11A is essential for Schwann cell activation and peripheral nerve regeneration, making it a potential therapeutic target for peripheral nerve injury treatment.
MOLECULAR NEUROBIOLOGY
(2023)
Article
Pharmacology & Pharmacy
Qingling Chen, Lili Zhao, Ling Mei, Xiaotong Zhao, Ping Han, Jie Liu, Chao Meng, Ruifang Li, Rui Zhong, Kai Wang, Jia Li
Summary: This study explored the intervention effect and mechanism of vitamin C (VC) and vitamin D-3 (VD3) on metabolic-associated fatty liver disease (MAFLD) using a high-fat diet-induced obese mouse model. The results showed that VC and VD3 improved gut-liver interactions by modulating gut microbiota composition and bile acid metabolism, leading to the amelioration of MAFLD.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Optics
Hai Wang, Juntao Tian, Lili Zhao, Zhiyong Li, Rongqing Tan
Summary: This study investigates the output characteristics of a ZGP optical parametric oscillator (OPO) over a wide temperature range. The temperature-dependent Sellmeier equation of the ZGP crystal was determined, and the temperature-tuned curves of the ZGP OPO at different phase matching angles were calculated. A tunable long-wave laser was obtained with a total tuning range of 1.39 μm. The laser has a wide tunable range and compact mechanical configuration, which is important for the development of long-wave lasers with a wide tuning range.
OPTICS COMMUNICATIONS
(2023)
Article
Robotics
Lili Zhao, Yangshan Tang, Leilei Wang
Summary: Based on camera calibration in the detection area, this study proposes an improved method of time interpolation for vehicle speed identification. The calibration of camera parameters is performed through preset rectangular detection area in photogrammetry. Key frames are extracted from video images, and target vehicle feature points and their image coordinates are identified. The actual coordinates of the characteristic points are determined through coordinate conversion. Finally, the improved time interpolation method, considering the acceleration between frames, is used to identify the vehicle speed, thereby improving the accuracy of the measurement. The results demonstrate that the vehicle speed calculated using the improved time interpolation method is more reliable, with a reduced error of 0.7%.
INTERNATIONAL JOURNAL OF ADVANCED ROBOTIC SYSTEMS
(2023)
Article
Cardiac & Cardiovascular Systems
Jichun Tang, Xuan Li, Lili Zhao, Jiajun Hui, Ning Ding
Summary: The study aimed to explore the biological functions and regulatory mechanism of circ_0006220 in non-small cell lung cancer (NSCLC). It was found that circ_0006220 was highly expressed in NSCLC tissues and cells. Silencing circ_0006220 inhibited the proliferation, migration, invasion, and angiogenesis of NSCLC cells, and induced cell apoptosis. Circ_0006220 acted as a sponge for miR-342-3p and upregulated the expression of GOT2 by sponging miR-342-3p, promoting the malignant behaviors of NSCLC cells.
ANNALS OF THORACIC AND CARDIOVASCULAR SURGERY
(2023)
Article
Biochemical Research Methods
Weijie Zhang, Yichu Shan, Lili Zhao, Zhen Liang, Chao Liu, Lihua Zhang, Yukui Zhang
Summary: Chemical cross-linking combined with mass spectrometry (CXMS) is a well-established method for profiling protein-protein interactions (PPIs). However, current structure-based analysis software faces challenges in large-scale analysis and lacks global measurement of dynamic structure mapping results. ComMap is a software that integrates CXMS data with existing structures to perform large-scale mapping, enabling fast distance calculation of PPIs and providing scores for testable hypothetical structural dynamism through a global view.
Article
Medicine, Research & Experimental
Yutao Zhang, Yongming Yang, Likun Zan, Jing Wang, Lei Yan, Lili Zhao, Lixia Chen, Yanfeng Xi, Wenqi Bai, Xihua Yang
Summary: This study successfully established a colorectal cancer PDX model and verified its applicability. Hematoxylin and eosin staining and immunohistochemical staining were used to evaluate the model. The established PDX model was used to study the efficacy of capecitabine in treating colorectal cancer.
IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES
(2023)