Review
Food Science & Technology
Jiawen Zhang, Haoqing Yang, Jing Li, Jiamiao Hu, Guanyuan Lin, Bee K. K. Tan, Shaoling Lin
Summary: Foodborne diseases caused by pathogens pose risks to food safety. Detecting and inactivating bacteria has been a hot topic in the field. However, bacteria can enter a viable but non-culturable state under adverse conditions, making detection challenging. This review summarizes the latest research on viable but non-culturable foodborne pathogens, including induction conditions, detection methods, formation mechanisms, and control strategies, aiming to provide ideas and methods for future research on these bacteria.
Article
Microbiology
Yang He, Junfeng Zhao, Hua Yin, Yuan Deng
Summary: The study found that beer-spoilage yeast can be induced into a viable but non-culturable state by treatment with hop bitter acids, and can be recovered to a culturable state within a short period of time by the addition of catalase. Transcriptome profiling showed that certain genes were downregulated during the viable but non-culturable state, while others were upregulated during the recovery process.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Sooyeon Song, Thomas K. Wood
Summary: Most bacteria enter sleep mode to survive stress, while a small portion of cells become dormant in response to continued stress. Under prolonged pressure, these cells become spheroid and eventually die, forming hollow, membrane-enclosed structures. The process through which cell content is lost remains unclear, but there is debate among scientists regarding whether these dead cell husks can be reborn.
ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Immunology
Leticia Silva Santos, Daise Aparecida Rossi, Raquelline Figueiredo Braz, Belchiolina Beatriz Fonseca, Micaela Guidotti-Takeuchi, Rosiane Nascimento Alves, Marcelo Emilio Beletti, Hebreia Oliveira Almeida-Souza, Larissa Prado Maia, Paula de Souza Santos, Jessica Brito de Souza, Roberta Torres de Melo
Summary: Despite being considered fragile and fastidious, Campylobacter jejuni is the most prevalent cause of foodborne bacterial gastroenteritis, especially through chicken meat transmission. Extreme stress can induce the acquisition of the viable but not culturable (VBNC) state in C. jejuni, which demonstrates its adaptability and virulence. The presence of transcripts, cell lysis, and specific metabolites in the VBNC form further emphasize the potential danger and difficulty in detection of this latent pathogen.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Corinna Lieleg, Ana Novacic, Sanja Musladin, Andrea Schmid, Goezde Gueclueler Akpinar, Slobodan Barbaric, Philipp Korber
Summary: Chromatin remodeling by ATP-dependent enzymes is crucial for genomic processes. The recruitment of remodelers, such as the SWI/SNF complex, in the removal of nucleosomes in yeast PHO gene induction is specific and may affect the outcome of remodeling.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Aurelie Lotoux, Eliane Milohanic, Helene Bierne
Summary: The VBNC state of bacteria, particularly in the case of Lm pathogens, has important implications for environmental, agricultural, and food industry settings, as well as potential risks in the host. The hypothesis that VBNC forms contribute to adaptation, persistence, and transmission of Lm between different ecological niches is put forth.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Microbiology
Hua Jiang, Kan Wang, Muxia Yan, Qian Ye, Xiaojing Lin, Ling Chen, Yanrui Ye, Li Zhang, Junyan Liu, Tengyi Huang
Summary: This study developed a rapid and highly sensitive cross-priming amplification (CPA) method for detecting important toxins in MRSA, in combination with a rapid VBNC cell detection method. The PMA-CPA detection was able to identify various toxins in MRSA in the VBNC state with an accuracy rate of 100%.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Biotechnology & Applied Microbiology
Laurens Maertens, Jean-Yves Matroule, Rob Van Houdt
Summary: Copper has antimicrobial applications in agriculture and healthcare, but can also induce bacteria to enter the VBNC state, posing a threat to plants and animals. Common observations in Cu-induced VBNC cells include specific cellular reactions and proteome reconfigurations, which may be adaptive responses to Cu toxicity.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2021)
Review
Microbiology
Nathan E. Wideman, James D. Oliver, Philip Glen Crandall, Nathan A. Jarvis
Summary: This article discusses the detection, enumeration, and virulence potential of VBNC pathogens, highlighting the importance of supplementing routine Lm testing methods with methods designed to enumerate VBNC cells. Five methods for enumerating VBNC Lm are summarized, with Live/Dead BacLight(TM) staining and CFDA-DVC staining appearing to be the most accurate. Understanding the impact of the VBNC state on the virulence of Lm and identifying environmental triggers that can cause Lm to become virulent are important for public health risk assessment.
Article
Microbiology
Sariqa Wagley, Helen Morcrette, Andrea Kovacs-Simon, Zheng R. Yang, Ann Power, Richard K. Tennant, John Love, Neil Murray, Richard W. Titball, Clive S. Butler
Summary: Members of the Proteobacteria can enter a viable but non culturable (VBNC) state as a survival strategy under stressful conditions. Using Vibrio parahaemolyticus as a model organism, distinct subpopulations in the VBNC state with different resuscitation potentials and proteomic profiles were identified. Deletion of the lactate dehydrogenase gene triggered cells to enter VBNC state, while lactate addition extended resuscitation potential. The ability to survive in VBNC state may be linked to overcoming oxidative stress.
Article
Biochemistry & Molecular Biology
Hsin-Yi Chang, Chin-Ying Gui, Tsui-Chin Huang, Yen-Con Hung, Tai-Yuan Chen
Summary: This study investigates the impact of slightly acidic electrolyzed water (SAEW) on Listeria monocytogenes and uncovers the molecular mechanisms leading to the viable but nonculturable (VBNC) state. Proteomic techniques were used to identify differentially expressed proteins and functional enrichment analysis revealed the affected pathways. The study also highlights the role of protein chlorination and its potential consequences. These findings have significant implications for food safety and provide potential strategies for controlling L. monocytogenes in the VBNC state.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Gustavo Cordero-Bueso, Ileana Vigentini, Roberto Foschino, David Maghradze, Marina Ruiz-Munoz, Francisco Benitez-Trujillo, Jesus M. Cantoral
Summary: This study focuses on the occurrence and diversity of culturable yeast in wild grape berries, identifying 49 species including the wine yeast Saccharomyces cerevisiae. It highlights the biodiversity potential in natural environments and its significance in winemaking.
Article
Environmental Sciences
Yulong Fu, Hongxi Peng, Jingqing Liu, Thanh H. Nguyen, Muhammad Zaffar Hashmi, Chaofeng Shen
Summary: This study quantified the culturability of waterborne pathogens in biofilms from a metropolitan drinking water distribution system, finding that pipe material significantly influences the culturability of pathogens and microbial communities. Stainless steel clad pipe was identified as a better choice for pathogen control compared to other metal pipes. These findings provide valuable insights into managing water quality in DWDS.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Acoustics
Su Jin Eom, Tae-Gyu Lim, Hyunjhung Jhun, Nam Hyouck Lee, Min-Cheol Kang, Kyung-Mo Song
Summary: The study developed a yeast extract capable of inhibiting melanoma cells using ultrasound technology, showing that it effectively reduces cell growth and proliferation, as well as tumor volume in mice. Optimal extraction conditions resulted in increased total extraction and 8-glucan yields, suggesting that UAEY may represent an effective anti-skin cancer agent.
ULTRASONICS SONOCHEMISTRY
(2021)
Article
Microbiology
Anusuya Debnath, Shin-ichi Miyoshi
Summary: Proteinase K can efficiently promote the recovery and growth of dormant VBNC cells at higher temperatures by decreasing the duration of the initial lag phase required for transitioning from the VBNC to recovery state and increasing the growth rate of these recovered cells.