A rapid detection of tomato yellow leaf curl virus using recombinase polymerase amplification-lateral flow dipstick assay

Tomato yellow leaf curl disease which is caused by Tomato yellow leaf curl virus (TYLCV) is economically important and a widely spread tomato disease in China. Rapid and accurate detection methods are important in the control TYLCV. Here, a rapid method was developed to identify TYLCV on the basis of recombinase polymerase amplification (RPA) that can be visualized in 5 min using lateral flow dipsticks. The sensitivity and the specificity of this method were evaluated. This method can detect 0 center dot 5 pg DNA after 30 min at 37 degrees C without any expensive instrumentation. In addition, it showed higher sensitivity than a PCR method when purified DNA was used. Moreover, the TYLCV was specifically detected, whereas other viruses infecting tomato produced negative results. The crude tomato extracts used in this assay has potential application in minimally equipped plant clinic laboratories. This method will facilitate the early and rapid detection of TYLCV for the timely application of control measures.

Automated summary

A rapid detection method for Tomato yellow leaf curl virus (TYLCV) has been developed, based on recombinase polymerase amplification (RPA) technology, with high sensitivity and specificity, visual results in 5 minutes without expensive equipment, showing potential application in minimally equipped plant clinic laboratories.