Article
Biotechnology & Applied Microbiology
Jing Liu, Keli Gao, Dingwei Li, Yang Zeng, Xueyang Chen, Xiongyan Liang, Chun Fang, Yufang Gu, Chunfeng Wang, Yuying Yang
Summary: This study demonstrated that the recombinant invasive Lactobacillus plantarum NC8 expressing Gp85 protein of ALV-J could enhance the immune responses in chickens, leading to increased protection against ALV-J infection. This provides a novel method for exploring live vaccines against ALV-J.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Liqin Liao, Zhiqiang Wu, Weiguo Chen, Huanmin Zhang, Aijun Li, Yiming Yan, Zi Xie, Hongxin Li, Wencheng Lin, Jingyun Ma, Xinheng Zhang, Qingmei Xie
Summary: Control of ALV-J in chicken breed is a serious issue that needs more attention. The elimination of vertical transmission of ALV-J is not yet well understood, and effective vaccines or drugs have not been developed. This study found that anti-CD81 antibody can inhibit viral infection and prevent vertical transmission of ALV-J in an animal model.
VETERINARY MICROBIOLOGY
(2022)
Article
Microbiology
Sheng Chen, Yiming Yan, Liguo Gao, Shuang Gao, Keyu Feng, Hongxin Li, Xinheng Zhang, Weiguo Chen, Feng Chen, Qingmei Xie
Summary: In this study, proteomic analysis revealed that 53 cellular proteins are incorporated into mature ALV-J virions, with potential functions in the viral life cycle and tumorigenesis. These findings provide new insights into the interaction between ALV-J and its host, and shed light on the cellular requirements for ALV-J infection.
VETERINARY MICROBIOLOGY
(2023)
Article
Veterinary Sciences
Moru Xu, Fusen Hang, Kun Qian, Hongxia Shao, Jianqiang Ye, Aijian Qin
Summary: This report describes a disease characterized by hepatomegaly and splenomegaly in chickens that is closely associated with a novel strain of ALV-J. Other viruses were ruled out as the cause of the disease, highlighting the importance of an ALV-J eradication program.
BMC VETERINARY RESEARCH
(2022)
Article
Agriculture, Dairy & Animal Science
Jun Zhang, Li Ma, Tuofan Li, Luyuan Li, Qiuqi Kan, Xiaohui Yao, Quan Xie, Zhimin Wan, Hongxia Shao, Aijian Qin, Jianqiang Ye
Summary: The study revealed that co-infection of CIAV and ALV-J caused significant body weight loss in infected chickens, with CIAV increasing the viral load of ALV-J in tissues. Furthermore, both CIAV and ALV-J significantly inhibited the humoral immunity response to specific viruses in infected chickens.
Article
Virology
Liqin Liao, Weiguo Chen, Xiangyu Zhang, Huanmin Zhang, Aijun Li, Yiming Yan, Zi Xie, Hongxing Li, Wencheng Lin, Jingyun Ma, Xinheng Zhang, Qingmei Xie
Summary: This study discovered a novel means of vertical transmission for ALV-J by utilizing host semen extracellular vesicles, and demonstrated the feasibility of this transmission method.
Article
Microbiology
Kunmei Yang, Jianhao Yang, Defang Zhou, Mingjun Zhu, Xusheng Du, Jing Zhou, Shenglong Liu, Ziqiang Cheng
Summary: In this study, the molecular mechanisms contributing to Avian leukosis virus subgroup J (ALV-J) infection were elucidated. It was found that ALV-J hijacked MIF through its p10 and p27 subunit proteins to facilitate infection. MIF overexpression and knockdown experiments as well as treatment with the MIF antagonist ISO-1 confirmed that MIF significantly promoted ALV-J internalization and replication. Additionally, the interaction between the subunit proteins p10 and p27 of Gag and MIF in the cytoplasm was observed. These findings provide insights into the roles of p10/p27 and the host factor MIF in ALV-J infection.
VETERINARY MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Yuan Chen, Hong-wei Li, Feng Cong, Yue-xiao Lian
Summary: This study used a nontargeted metabolomics approach to identify differential metabolites in plasma samples from ALV-J-infected chickens. The results showed significant alterations in metabolite levels in infected chickens compared to healthy controls, suggesting potential biomarkers for ALV-J-induced metabolic changes. These findings provide valuable insights into the metabolic effects of ALV-J infection.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Veterinary Sciences
Ning Cui, Xuezhi Cui, Qinghua Huang, Shaohua Yang, Shuai Su, Chuantian Xu, Jianhe Li, Wenfeng Li, Chao Li
Summary: The study isolated an ALV-J strain LH20180301 from broiler breeder chickens, exhibiting new genomic characters in terms of pathogenicity and oncogenicity, and branching with locally prevalent ALV-J strains in yellow chickens in South China.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Agriculture, Dairy & Animal Science
X. H. Wu, Z. Q. Yao, Q. Q. Zhao, S. Chen, Z. Z. Hu, Z. Xie, L. Y. Chen, J. Ji, F. Chen, X. H. Zhang, Q. M. Xie
Summary: In this study, a real-time fluorescent RT-RAA method was developed for rapid and specific detection of ALV-J in poultry. The method showed high sensitivity, good repeatability, and could complete the test within a short time. Moreover, there was no cross-reactivity with other avian leukosis virus subgroups. The study results indicated that this method is simple, easy to use, and suitable for clinical and laboratory diagnosis, providing technical support for the prevention and control of ALV-J.
Article
Virology
Dandan Qiao, Qian He, Xiaowei Cheng, Yongxiu Yao, Venugopal Nair, Hongxia Shao, Aijian Qin, Kun Qian
Summary: This study investigated the role of the Wnt/beta-catenin signaling pathway during avian leukosis virus subgroup J (ALV-J) infection. The results showed that activation of this pathway increased viral replication, while inhibition significantly reduced virus titer and gene expression.
Article
Virology
Qiaomu Deng, Min Li, Chengwei He, Qiaoe Lu, Yanli Gao, Qiuhong Li, Mengya Shi, Peikun Wang, Ping Wei
Summary: Avian leukosis virus subgroup J (ALV-J) has caused significant economic losses in China by infecting various birds. In-depth analysis of the genetic diversity of ALV-J revealed that current Chinese strains are mainly in Clade 1.3, while Chinese and Egyptian chicken flocks are encountering emerging Clade 2 viruses. The proposed unified classification system for ALV-J will facilitate future studies on epidemiology, genetic evolution, and sequence comparison worldwide.
Article
Veterinary Sciences
Lan Wu, Youjun Li, Xueyang Chen, Yuxin Yang, Chun Fang, Yufang Gu, Jing Liu, Xiongyan Liang, Yuying Yang
Summary: This study characterized the genome of the ALV-J strain HB2020 and found it to be a recombinant strain with mutations in the gag gene compared to the prototype strain HPRS-103. Animal experiments showed that HB2020 could cause various neoplastic lesions in chickens.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Agriculture, Dairy & Animal Science
Houkun Wang, Xueyang Chen, Lilin Zhu, Xiaowei Fang, Keli Gao, Chun Fang, Jing Liu, Yufang Gu, Xiongyan Liang, Yuying Yang
Summary: This study successfully prepared a monoclonal antibody specific for ALV-J and identified a new epitope of ALV-J Gp85. This finding may assist in the development of specific ALV-J detection methods.
Article
Microbiology
Ahmed Kheimar, Romina Klinger, Luca D. Bertzbach, Hicham Sid, You Yu, Andele M. Conradie, Benjamin Schade, Brigitte Boehm, Rudolf Preisinger, Venugopal Nair, Benedikt B. Kaufer, Benjamin Schusser
Summary: This study demonstrates that genetically modified chickens with a mutation of the chNHE1 receptor are resistant to highly pathogenic ALV-J infection, providing a potential strategy to combat this pathogen and improve animal health.
Article
Infectious Diseases
Gen Li, Xusheng Du, Defang Zhou, Chengui Li, Libo Huang, Qiankun Zheng, Ziqiang Cheng
TRANSBOUNDARY AND EMERGING DISEASES
(2018)
Article
Virology
Jing Zhou, Guo-Liang Zhao, Xiao-Man Wang, Xu-Sheng Du, Shuai Su, Chen-Gui Li, Venugopal Nair, Yong-Xiu Yao, Zi-Qiang Cheng
Article
Virology
Shuhai He, Gaoying Zheng, Defang Zhou, Gen Li, Mingjun Zhu, Xusheng Du, Jing Zhou, Ziqiang Cheng
Article
Infectious Diseases
Defang Zhou, Jingwen Xue, Ya Zhang, Guihua Wang, Yongsheng Feng, Liping Hu, Yingli Shang, Ziqiang Cheng
TRANSBOUNDARY AND EMERGING DISEASES
(2019)
Article
Immunology
Dongjin Zhu, Defang Zhou, Jie Liu, Xiaojing Hao, Ziqiang Cheng
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
(2019)
Article
Cell Biology
Mingjun Zhu, Jing Zhou, Yanfei Liang, Venugopal Nair, Yongxiu Yao, Ziqiang Cheng
JOURNAL OF LEUKOCYTE BIOLOGY
(2020)
Article
Infectious Diseases
Yisong Zhu, Junhong Liu, Kaiyan Guo, Jianhua Qiu, Ziqiang Cheng, Faxiao Liu, Yuqing Zhao, Dingxiu Zhang, Huijun Guo, Hongmei Li
Summary: This study reports an outbreak of a new disease caused by Staphylococcus pseudintermedius in raccoon dogs in a breeding farm in Guan County, Shandong Province, China in August 2019. The pathogen was identified as S. pseudintermedius, leading to skin and soft tissue infections in affected dogs, with some cases resulting in death.
TRANSBOUNDARY AND EMERGING DISEASES
(2021)
Article
Microbiology
Shiyu Yuan, Tianxing Yan, Libo Huang, Xiaojing Hao, Manda Zhao, Shicheng Zhang, Defang Zhou, Ziqiang Cheng
Summary: The study established chicken and mouse models of GyV3 infection, showing that GyV3 induced persistent infections with characteristics of viremia, aplastic anemia, immunosuppression, and systematic lymphocytic inflammation.
VETERINARY MICROBIOLOGY
(2021)
Article
Immunology
Xusheng Du, Defang Zhou, Jing Zhou, Jingwen Xue, Ziqiang Cheng
Summary: Co-infection of Marek's disease virus (MDV) and reticuloendotheliosis virus (REV) synergistically enhances disease progression via the RIOK3-Akt pathway, leading to increased replication of both viruses.
Article
Cell Biology
Defang Zhou, Longying Ding, Menglu Xu, Xiaoyao Liu, Jingwen Xue, Xinyue Zhang, Xusheng Du, Jing Zhou, Xiyao Cui, Ziqiang Cheng
Summary: In this study, it was found that ALV-J and REV synergistically promoted mutual replication, suppressed cellular senescence, and activated epithelial-mesenchymal transition (EMT) in vitro. The mechanism involves the activation of Musashi-1 (MSI1) by the structural proteins of ALV-J and REV, which inhibits the maturation of miR-147, relieving the inhibition of NF-KB/KIAA1199/EGFR signaling and leading to cellular senescence suppression and EMT activation.
Article
Virology
Jingwen Xue, Defang Zhou, Jing Zhou, Xusheng Du, Xinyue Zhang, Xiaoyang Liu, Longying Ding, Ziqiang Cheng
Summary: Co-infection of avian leukosis virus subgroup J (ALV-J) and reticuloendotheliosis virus (REV) in poultry flocks induces synergistic replication, which worsens immunosuppression, growth inhibition, and increased mortality. MicroRNA-155 (miR-155) targets a dual pathway, PRKCI-MAPK8 and TIMP3-MMP2, which interacts with the U3 region of ALV-J and REV to promote synergistic replication between ALV-J and REV.
JOURNAL OF VIROLOGY
(2023)
Article
Veterinary Sciences
Shuhai He, Gaoying Zheng, Defang Zhou, Li Huang, Jianguo Dong, Ziqiang Cheng
Summary: Congenital avian leukosis virus subgroup J (ALV-J) infection induces persistent immunotolerance in chickens by increasing the frequency and activation of CD4(+)CD25(+) Tregs, which express inhibitory factors to suppress B cell functions. The infection also leads to immune organ dysplasia and significant fluctuations in cytokine levels, contributing to the maintenance of immunotolerance in ALV-J-infected chickens.
VETERINARY RESEARCH
(2021)
Article
Veterinary Sciences
Gen Li, Defang Zhou, Manda Zhao, Qing Liu, Xiaojing Hao, Tianxing Yan, Shiyu Yuan, Shicheng Zhang, Ziqiang Cheng
Summary: Gyrovirus 3 (GyV3) induces persistent infection in SPF chickens, leading to diarrhea, aplastic anemia, immunosuppression, and persistent systemic lymphocytic inflammation. Clinically, infected chickens show symptoms like ruffled feathers, diarrhea, anemia, and weight loss.
VETERINARY RESEARCH
(2021)
Article
Veterinary Sciences
Ling Li, Pingping Zhuang, Ziqiang Cheng, Jie Yang, Jianmin Bi, Guihua Wang
JOURNAL OF VETERINARY SCIENCE
(2020)