Letter
Immunology
Himali S. Jayasinghearachchi, Vaithehi R. Francis, Harindra D. Sathkumara, Shivankari Krishnananthasivam, Jayanthi Masakorala, Thilini Muthugama, Aruna D. De Silva, Enoka M. Corea
Summary: A cluster of melioidosis cases occurred in eastern Sri Lanka, with four infections caused by Burkholderia pseudomallei isolates of sequence type 594. Whole-genome analysis revealed genetic diversity among the isolates and showed that the cluster was nonclonal.
EMERGING INFECTIOUS DISEASES
(2021)
Review
Immunology
Vanitha Mariappan, Kumutha Malar Vellasamy, Muttiah Barathan, A. S. Smiline Girija, Esaki M. Shankar, Jamuna Vadivelu
Summary: B. pseudomallei infection causes melioidosis with varying severity, diverse pathological manifestations, complex mechanisms of latency and relapse, and the crucial role of immune response in eradicating the pathogen. However, the question of why some individuals are asymptomatic while others are life-threatening remains unanswered.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Microbiology
Kasturi Selvam, Muhammad Fazli Khalid, Khairul Mohd Fadzli Mustaffa, Azian Harun, Ismail Aziah
Summary: Melioidosis is a severe disease caused by Burkholderia pseudomallei, mainly affecting immunocompromised populations such as patients with type 2 diabetes mellitus. Laboratory diagnosis is challenging due to non-specific clinical manifestations and the imperfect sensitivity of culture methods.
Article
Microbiology
Delaney Burnard, Gemma Robertson, Andrew Henderson, Caitlin Falconer, Michelle J. Bauer, Kyra Cottrell, Ian Gassiep, Robert Norton, David L. Paterson, Patrick N. A. Harris
Summary: Cefiderocol, a cephalosporin designed to treat multidrug-resistant Gram-negative infections, exhibits strong in vitro activity against Burkholderia pseudomallei isolates. With promising results in laboratory testing, this compound shows potential as a treatment option for melioidosis in endemic regions.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Multidisciplinary Sciences
Sandra Appelt, Anna-Maria Rohleder, Daniela Jacob, Heiner Von Buttlar, Enrico Georgi, Katharina Mueller, Ulrich Wernery, Joerg Kinne, Marina Joseph, Shantymol V. Jose, Holger C. Scholz
Summary: This study developed a core genome-based Multi Locus Sequence Typing Assay (cgMLST) for the typing and identification of the etiological agent of glanders, Burkholderia mallei. The assay has high resolution and can distinguish between strains from different geographic regions and epidemiologically related strains based on allele differences. It is robust and reliable, and can be used for outbreak investigations and epidemiological studies.
Article
Infectious Diseases
Yanshuang Wang, Xuemiao Li, David A B Dance, Han Xia, Chen Chen, Nini Luo, Anyang Li, Yanmei Li, Qiao Zhu, Qinghui Sun, Xingyong Wu, Yingfei Zeng, Lin Chen, Shen Tian, Qianfeng Xia
Summary: The study evaluated the clinical potential of Burkholderia pseudomallei phages isolated from Hainan, China, for treating melioidosis. Results showed that the phage exhibited promising lytic properties and stability, significantly improving cell and nematode survival rates.
INFECTIOUS DISEASES OF POVERTY
(2022)
Article
Microbiology
Daniel Tapia, Javier I. Sanchez-Villamil, Heather L. Stevenson, Alfredo G. Torres
Summary: The study introduces an elegant multicomponent glycoconjugate vaccine formulation capable of eliciting both humoral and cell-mediated responses against lethal B. pseudomallei challenge.
Article
Immunology
Quyen T. L. Tran, Phuc H. Phan, Linh N. H. Bui, Ha T. Bui, Ngoc T. B. Hoang, Dien M. Tran, Trung T. Trinh
Summary: Within 8 months, 3 children from 1 family in northern Vietnam died from melioidosis caused by Burkholderia pseudomallei. The same sequence type, 541, was isolated from clinical samples, borehole water, and garden and rice field soil.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Microbiology
Christopher P. P. Klimko, Jennifer L. L. Shoe, Nathaniel O. O. Rill, Melissa Hunter, Jennifer L. L. Dankmeyer, Yuli Talyansky, Lindsey K. K. Schmidt, Caitlyn E. E. Orne, David P. P. Fetterer, Sergei S. S. Biryukov, Mary N. N. Burtnick, Paul J. J. Brett, David DeShazer, Christopher K. K. Cote
Summary: Researchers have found that combining current vaccine strategies and co-trimoxazole regimens can provide near uniform protection against melioidosis. This combinatorial strategy shows significant improvement in several antibiotic regimens and works similarly with both protein subunit and live attenuated vaccines.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Microbiology
Muhammad Shafiq, Bixia Ke, Xin Li, Mi Zeng, Yumeng Yuan, Dongmei He, Xiaoling Deng, Xiaoyang Jiao
Summary: The study identified high genetic diversity among 8 clinical Burkholderia pseudomallei isolates, which were susceptible to commonly used antibiotics for melioidosis treatment. Various antibiotic-resistant genes and mutation factors, as well as 165 virulence factors were discovered, indicating different pathogens responsible for various disorders. Phylogenetic analysis showed diverse strains with implications for disease management.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Anna Di Fiore, Viviana De Luca, Emma Langella, Alessio Nocentini, Martina Buonanno, Simona Maria Monti, Claudiu T. Supuran, Clemente Capasso, Giuseppina De Simone
Summary: This paper presents a detailed characterization of BpsyCA, a y-carbonic anhydrase from Burkholderia pseudomallei, highlighting its catalytic activity, crystal structure, and the role of residues in its catalytic mechanism and ligand recognition. These findings provide an important starting point for the development of new anti-melioidosis drugs targeting BpsyCA.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Review
Immunology
Nicole M. Bzdyl, Clare L. Moran, Justine Bendo, Mitali Sarkar-Tyson
Summary: Burkholderia pseudomallei, a soil saprophyte, is responsible for melioidosis, a prevalent disease in Southeast Asia and northern Australia. This review highlights the various mechanisms employed by B. pseudomallei to survive and spread within host cells.
Article
Veterinary Sciences
Himali S. Jayasinghearachchi, Thilini A. Muthugama, Jayanthi Masakorala, Upeksha S. Kulasekara, Kumari Jayaratne, D. A. Dasun N. Jayatunga, Aruna D. De Silva, Enoka M. Corea
Summary: This study provides evidence for the presence of Burkholderia pseudomallei in soil and water in Sri Lanka. It highlights the risk of melioidosis for populations using contaminated water sources and cultivating crops in these areas. Identifying positive sites can help raise public awareness and facilitate remediation efforts.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Infectious Diseases
Cindy Shuan Ju Teh, Polly Soo Xi Yap, Noorfatin Jihan Zulkefli, Prasanna Subramaniam, Pik San Sit, Zhi Xian Kong, Chun Wie Chong, Kumutha Malar Vellasamy, Vanitha Mariappan, Sasheela Ponnampalavanar, Jamuna Vadivelu
Summary: The study conducted MLST analysis on B. pseudomallei strains in Malaysia, revealing a high diversity of sequence types and indicating localized evolution giving rise to new STs, with host and environmental factors playing a crucial role in the evolutionary changes of B. pseudomallei.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Medicine, General & Internal
Noorah Alsowayeh, Aqel Albutti
Summary: This study developed a multi-epitope-based vaccine against Burkholderia pseudomallei using advanced bacterial pan-genome analysis. The constructed vaccine showed a high population coverage of 99.74% and potential binding efficacy with human toll-like receptors-5. Molecular dynamics simulation also suggested a stable immunological response within the host body. This in-silico vaccine design could be valuable for formulating a highly effective vaccine for B. pseudomallei.
FRONTIERS IN MEDICINE
(2022)
Review
Neurosciences
Sudipta Patra, Vandana Kalwaje Eshwara, Aparna Ramakrishna Pai, Muralidhar Varma, Chiranjay Mukhopadhyay
Summary: Neurobrucellosis is a rare but clinically significant disease characterized by fever, headache, neurological symptoms, and altered sensorium. Early and accurate diagnosis is crucial for preventing complications associated with neurobrucellosis.
INTERNATIONAL JOURNAL OF NEUROSCIENCE
(2022)
Review
Pharmacology & Pharmacy
Prashant Chandra, M. K. Unnikrishnan, Vandana Ke, Chiranjay Mukhopadhyay, Dinesh U. Acharya, Surulivel M. Rajan, V Rajesh
Summary: Antimicrobial resistance (AMR) is a complex issue with catastrophic impacts on humans, livestock, the environment, and the biosphere. Initiatives and action plans to prevent AMR are poorly implemented in India. It is essential for healthcare professionals, hospitals, and the general public to cooperatively implement the 'One Health approach' of judicious antibiotic use.
EXPERT OPINION ON DRUG SAFETY
(2021)
Letter
Immunology
Yamuna Devi Bakthavatchalam, Chiranjay Mukhopadhyay, Balaji Veeraraghavan
INDIAN JOURNAL OF MEDICAL MICROBIOLOGY
(2022)
Article
Infectious Diseases
Rahul Garg, Chaitanya Tellapragada, Tushar Shaw, Vandana Kalwaje Eshwara, Vishal Shanbhag, Shwethapriya Rao, Harjeet S. Virk, Muralidhar Varma, Chiranjay Mukhopadhyay
Summary: The study aimed to elucidate the epidemiology of sepsis in a South Indian university hospital, focusing on common bacterial causes, MDR infection proportions, and risk factors for 28-day mortality. Male gender, hypertension, SOFA > 9, and elevated creatinine values were identified as predictors of mortality. Nearly half of cases did not have a detected infectious cause using routine microbiology culture methods, highlighting the need for increased awareness of early sepsis diagnosis in community settings.
INFECTIOUS DISEASES
(2022)
Article
Public, Environmental & Occupational Health
Omika Katoch, Sonal Katyal, Sharad Srivastav, Camilla Rodrigues, Priscilla Rupali, Arunaloke Chakrabarti, Pallab Ray, Vibhor Tak, Manisha Biswal, Reema Nath, Chiranjay Mukhopadhyay, Sanjay Bhattacharya, Kanne Padmaja, Vijayshri Deotale, Vimala Venkatesh, Chand Wattal, Thirunarayan Ma, Vijaya Lakshmi Nag, Raja Ray, Bijayini Behera, Sujata Baveja, Tadepalli Karuna, Sanjeev K. Singh, Bashir Fomda, Sulochana Khumanthem Devi, Padma Das, Neeta Khandelwal, Prachi Verma, Rajni Gaind, Prithwis Bhattacharyya, Puneet Kumar, Ashutosh Srivastava, Jyoti Iravane, Tapan Majumdar, Rajni Sharma, Dhanapaul Sankara, Vandana Kalwaje Eshwara, Muralidhar Varma, Shaista Nazir, Swagata Tripathy, Satyajeet Mishra, Anupam Dey, Premkumar Thangavelu, Lata Kapoor, Rajesh Malhotra, Kamini Walia, Randeep Guleria, Purva Mathur
Summary: The IPC practices across the network of Indian Hospitals were assessed using the IPCAF tool, with 13% having basic practices, 28% intermediate practices, and 59% advanced practices. There is room for improvement in quality improvement training for IPC nurses and healthcare professionals.
AMERICAN JOURNAL OF INFECTION CONTROL
(2022)
Article
Public, Environmental & Occupational Health
Michael G. Walsh, Amrita Pattanaik, Navya Vyas, Deepak Saxena, Cameron Webb, Shailendra Sawleshwarkar, Chiranjay Mukhopadhyay
Summary: This study investigated the landscape epidemiology of Japanese encephalitis virus (JEV) outbreaks in India from 2010 to 2020. The results show that outbreak risk is influenced by the habitat suitability of ardeid birds, pig and chicken density, and the shared landscapes between rain-fed agriculture and river and freshwater marsh wetlands.
INTERNATIONAL JOURNAL OF EPIDEMIOLOGY
(2022)
Article
Infectious Diseases
Chaitanya Tellapragada, Karin Andersson Ydsten, Anders Ternhag, Christian G. Giske
Summary: This study investigated the concordance between the Unyvero Hospitalized Pneumonia application and quantitative culture in detecting bacterial pathogens from consecutive lower respiratory tract specimens. The false positive rate of the HPN application was 29% and 10% in the two evaluation approaches, respectively. Additional pathogens detected by the HPN application were confirmed by culture positivity in previous or subsequent samples.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2022)
Article
Immunology
Madhura Punekar, Manas Kshirsagar, Chaitanya Tellapragada, Kanchankumar Patil
Summary: This review discusses the current landscape of antiviral drug repurposing for COVID-19 and its impact on the nervous system, highlighting the potential neurological toxicity associated with specific antiviral therapy.
MICROBIAL PATHOGENESIS
(2022)
Review
Infectious Diseases
Ruby Benson, Mazhuvancherry Kesavan Unnikrishnan, Shilia Jacob Kurian, Saleena Ummer Velladath, Gabriel Sunil Rodrigues, Raghu Chandrashekar Hariharapura, Anju Muraleedharan, Dinesh Bangalore Venkateshiah, Barnini Banerjee, Chiranjay Mukhopadhyay, Aieshel Serafin Johnson, Murali Munisamy, Mahadev Rao, Benson Mathai Kochikuzhyil, Sonal Sekhar Miraj
Summary: Infections are becoming more difficult to treat due to microbes producing biofilms. Vitamin D deficiency is associated with decreased antimicrobial peptides, increased inflammatory cytokines, and biofilm formation, making deficient individuals more susceptible to infection. Adjuvant therapy with vitamin D can help manage infections caused by biofilm-producing microbes due to its immunomodulatory role in inducing antimicrobial peptides and reducing proinflammatory cytokines.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2023)
Article
Biotechnology & Applied Microbiology
Thripthi Ananda, Ankita Modi, Vishwanath Managuli, Chiranjay Mukhopadhyay
Summary: The importance of the hospital environment in infection prevention and control has been widely recognized. Inappropriate antibiotic use has led to the emergence of drug-resistant strains that are difficult to treat. Nanotechnology has enhanced the antimicrobial properties of nanoparticles, overcoming the limitations of common antimicrobials. This study investigated the antimicrobial role of silver nanoparticles (Ag-NPs) against clinically important bacterial strains and observed the effects of varying storage temperatures on Ag-NPs' antimicrobial activity.
JOURNAL OF PURE AND APPLIED MICROBIOLOGY
(2023)
Article
Infectious Diseases
Chaitanya Tellapragada, Helena Ostlund, Christian Giske, Magnus Rasmussen, Andreas Berge
Summary: This study aimed to investigate the clinical presentation of patients with Enterococcus faecalis bacteremia (EfsB), focusing on the risk of recurrent infection and infective endocarditis (IE), as well as identifying potential improvements in management. Retrospective analysis of a cohort of patients with monomicrobial EfsB episodes revealed 69 patients with IE and 43 with recurrent infections. Factors significantly associated with IE included prolonged symptoms, growth in all blood cultures, unknown origin of infection, heart murmur, and predisposition for IE. Whole genome sequencing and multilocus sequence typing of E. faecalis isolates from multiple episodes in the same patient showed identical sequence types in 28 of 31 patients. Episodes of EfsB in patients later diagnosed with IE displayed characteristics of IE already in the first episodes and were likely true relapses. Risk factor analysis should guide the use of echocardiography.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2023)
Article
Public, Environmental & Occupational Health
Purva Mathur, Paul Malpiedi, Kamini Walia, Padmini Srikantiah, Sunil Gupta, Ayush Lohiya, Arunaloke Chakrabarti, Pallab Ray, Manisha Biswal, Neelam Taneja, Priscilla Rupali, Veeraraghavan Balaji, Camilla Rodrigues, Vijaya Lakshmi Nag, Vibhor Tak, Vimala Venkatesh, Chiranjay Mukhopadhyay, Vijayshri Deotale, Kanne Padmaja, Chand Wattal, Sanjay Bhattacharya, Tadepalli Karuna, Bijayini Behera, Sanjeev Singh, Reema Nath, Raja Ray, Sujata Baveja, Bashir A. Fomda, Khumanthem Sulochana Devi, Padma Das, Neeta Khandelwal, Prachi Verma, Prithwis Bhattacharyya, Rajni Gaind, Lata Kapoor, Neil Gupta, Aditya Sharma, Daniel VanderEnde, Valan Siromany, Kayla Laserson, Randeep Guleria
Summary: A standardized healthcare-associated infections (HAIs) surveillance network has been established in India, successfully implementing locally adapted protocols and identifying a large number of HAIs. The network data shows high rates of HAIs and antimicrobial resistance in tertiary hospitals, emphasizing the importance of implementing multimodal strategies for HAI prevention and antimicrobial resistance containment.
LANCET GLOBAL HEALTH
(2022)
Article
Infectious Diseases
Indu Ramachandra Rao, Tushar Shaw, Ravindra Attur Prabhu, Vandana Kalwaje Eshwara, Shankar Prasad Nagaraju, Dharshan Rangaswamy, Srinivas Vinayak Shenoy, Mohan Varadanayakanahalli Bhojaraja, Chiranjay Mukhopadhyay
Summary: Hyponatremia is common in patients with melioidosis, and its presence, especially in severe forms, is associated with higher in-hospital mortality, need for mechanical ventilation, and ICU stay.
JOURNAL OF GLOBAL INFECTIOUS DISEASES
(2022)
Article
Biotechnology & Applied Microbiology
Thripthi Ananda, Ankita Modi, Ishita Chakraborty, Vishwanath Managuli, Chiranjay Mukhopadhyay, Nirmal Mazumder
Summary: This review discusses the concept and major pathogens of hospital-acquired infections (HAIs). Due to the increasing antibacterial resistance, nanotechnology offers new solutions for detecting and treating bacterial infections. The paper also focuses on the HAI situation in India and highlights the significant initiatives taken by medical bodies and hospitals in raising awareness and providing training.
BIOENGINEERING-BASEL
(2022)
