Article
Multidisciplinary Sciences
Tiantian Zheng, Anton Zilman
Summary: The nuclear pore complexes (NPCs) in eukaryotic cells are responsible for macromolecular transport in and out of the nucleus. The NPCs contain intrinsically disordered proteins (IDPs) and efficiently transport cargoes in crowded conditions. A computational model of the NPC shows that self-regulating mechanisms and the spatial architecture of the IDP assembly contribute to efficient transport.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Cell Biology
Joanna Kalita, Larisa E. Kapinos, Roderick Y. H. Lim
Summary: This review examines the role of the nuclear pore complex in nucleocytoplasmic transport and discusses unresolved issues such as the partitioning of importin and exportin in the cytoplasm and nucleus, and the maintenance of the RanGTP-RanGDP gradient across the nuclear envelope.
JOURNAL OF CELL SCIENCE
(2021)
Review
Chemistry, Multidisciplinary
Shigeru Amemiya
Summary: The NPC is a proteinaceous nanopore that mediates molecular transport between the nucleus and cytoplasm in eukaryotic cells, regulating gene expression. Our research highlights the importance of chemistry in understanding and controlling NPC-mediated molecular transport for efficient genetic therapeutics delivery into the nucleus, contributing to human health. We use nanoscale scanning electrochemical microscopy to study how the NPC nanopore is divided into peripheral and central routes to facilitate protein transport and RNA export through hydrophobic and electrostatic interactions.
Review
Biochemistry & Molecular Biology
America Chandia Cristi, Sampath Rapuri, Alyssa N. Coyne
Summary: Nuclear pore complexes (NPCs) are crucial for maintaining nuclear-cytoplasmic balance and proper nuclear organization, and perturbations in their regulation are implicated in neurodegenerative diseases. The ESCRT-III pathway plays a critical role in the surveillance and preservation of functional NPCs and nuclear envelope sealing. Maintaining quality control of the nuclear envelope and NPCs is pivotal in the pathogenesis of neurodegenerative diseases.
Review
Cell Biology
Coby Rush, Zecheng Jiang, Mark Tingey, Fiona Feng, Weidong Yang
Summary: The nuclear pore complex (NPC) is a crucial subcellular structure that regulates nucleocytoplasmic transport through a selectively permeable barrier. Recent technological advancements have deepened our understanding of the NPC's architecture and operations. However, the presence of intrinsically disordered regions within nucleoporins (Nups) presents challenges to conventional static characterization techniques. This review provides a comprehensive overview of prominent models that aim to elucidate the structural layout and functional significance of Nups, highlighting their proposed dynamic and structural attributes supported by research.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jiaojiao Wang, Gaofeng Pei, Yupei Wang, Dewei Wu, Xiaokang Liu, Gaoming Li, Jianfang He, Xiaolin Zhang, Xiaoyi Shan, Pilong Li, Daoxin Xie
Summary: This study revealed that the phase separation of the nuclear pore complex (NPC) central barrier plays a crucial role in regulating plant defense against various biotic stresses. The NUP62 gene was found to positively regulate plant defense against the plant pathogen Botrytis cinerea. Further investigation showed that the phase separation of the NPC central barrier regulates the nucleocytoplasmic transport of immune regulators and activates plant defense against a broad range of biotic stresses.
Article
Biology
Luke K. Davis, Ian J. Ford, Bart W. Hoogenboom
Summary: The rapid transport of biological material to and from the cell nucleus is regulated by the nuclear pore complex (NPC), with a core permeability barrier consisting of FG Nups. Nuclear transport receptors (NTRs) facilitate transport by partitioning in the FG Nup assembly and make up a significant portion of proteins in the NPC barrier. Negative cooperativity was observed in the binding behavior of well-characterized NTRs, NTF2 and Imp-beta, to different planar assemblies of FG Nups, suggesting potential demixed phases of NTRs within the FG Nup assembly that could impact inter-NTR competition and separate transport pathways.
Article
Cell Biology
Kenneth D. Belanger, William T. Yewdell, Matthew F. Barber, Amy N. Russo, Mark A. Pettit, Emily K. Damuth, Naveen Hussain, Susan J. Geier, Karyn G. Belanger
Summary: This study demonstrates the importance of the exportin Crm1 in regulating the nucleocytoplasmic localization and activity of the transcription factor Swi6. The authors identify a putative nuclear export signal for Crm1 within Swi6 and show that export by Crm1 or another exportin, Msn5, independently influences the expression of different subsets of Swi6-controlled genes. These findings provide new insights into the complex regulation of Swi6 transcription activation activity and the role of nucleocytoplasmic shuttling in gene expression.
BMC MOLECULAR AND CELL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Wanlu Zhang, Azqa Khan, Jlenia Vitale, Annett Neuner, Kerstin Rink, Christian Luechtenborg, Britta Bruegger, Thomas H. Soellner, Elmar Schiebel
Summary: The short amphipathic alpha-helix of Apq12 regulates the function of Brl1 and Brr6, leading to defects in NPC biogenesis and NE integrity when disrupted. Overexpression of Apq12 promotes over-proliferation of the ONM/ER and accumulation of phosphatidic acid at the NE, possibly during NPC biogenesis.
Article
Cell Biology
Sandeep Kumar Dubey, Kirstin Maulding, Hyun Sung, Thomas E. Lloyd
Summary: This study found that expression of GGGGCC repeats in Drosophila neurons leads to degradation of nucleoporins and this process is mediated by the Vps4 ATPase and the ESCRT-III complex, revealing a mechanism for nuclear pore complex dysfunction in neurodegenerative diseases.
Review
Clinical Neurology
Olivia Spead, Benjamin L. Zaepfel, Jeffrey D. Rothstein
Summary: The nuclear pore complex (NPC) is a large multimeric structure that plays important roles in nucleocytoplasmic transport and cellular function. Dysfunction of NPC is associated with neurodegenerative disorders and can lead to neuronal loss.
Review
Plant Sciences
Daniel Ludke, Philipp F. W. Rohmann, Marcel Wiermer
Summary: Plant viability and responses to changing environments depend on spatial communication between the compartments separated by the nuclear envelope, involving bidirectional exchange of proteins and RNAs mediated by a sophisticated transport machinery.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Julie Jacquemyn, Joyce Foroozandeh, Katlijn Vints, Jef Swerts, Patrik Verstreken, Natalia Gounko, Sandra F. Gallego, Rose Goodchild
Summary: Torsin affects lipid metabolism by regulating NEP1R1-CTDNEP1 and Lipin, influencing nuclear envelope remodeling and nuclear pore complex biogenesis. This mechanism explains some of the pleiotropic effects of Torsins.
Article
Chemistry, Multidisciplinary
Qi Shen, Taoran Tian, Qiancheng Xiong, Patrick D. Ellis Fisher, Yong Xiong, Thomas J. Melia, C. Patrick Lusk, Chenxiang Lin
Summary: Researchers have developed a biomimetic construct called NanoTrap using DNA nanotechnology to better understand how nucleoporins in the nuclear pore complex establish a selective barrier. The type of FG motif, grafting density, and spatial arrangement were found to be critical determinants of an effective diffusion barrier, with diffusion barriers formed with cohesive FG interactions dominating in mixed-FG-nup scenarios.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Review
Plant Sciences
Daniel Luedke, Qiqi Yan, Philipp F. W. Rohmann, Marcel Wiermer
Summary: This article discusses the role of nucleoporins and nuclear transport receptors (NTRs) in plant immunity, with a focus on NLR immune signaling across the nuclear compartment boundary and within the nucleus. The authors also highlight the cytoplasmic and nuclear functions of NLRs and their signaling partners, as well as the potential implications of NLR activation and resistosome formation in both cellular compartments for mediating plant pathogen resistance and programmed host cell death.
Article
Cell Biology
Suncica Barbato, Larisa E. Kapinos, Chantal Rencurel, Roderick Y. H. Lim
JOURNAL OF CELL SCIENCE
(2020)
Article
Multidisciplinary Sciences
Christina Zelmer, Ludovit P. Zweifel, Larisa E. Kapinos, Ioana Craciun, Zekiye P. Guven, Cornelia G. Palivan, Roderick Y. H. Lim
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Multidisciplinary Sciences
Jose Carlos Santos, Dave Boucher, Larisa Kapinos Schneider, Benjamin Demarco, Marisa Dilucca, Kateryna Shkarina, Rosalie Heilig, Kaiwen W. Chen, Roderick Y. H. Lim, Petr Broz
NATURE COMMUNICATIONS
(2020)
Article
Chemistry, Physical
Deepika Sharma, Roderick Y. H. Lim, Thomas Pfohl, Yasin Ekinci
Summary: Utilizing geometry-induced electrostatic (GIE) potential trap in a nanofluidic device is an efficient and robust method for nanoparticle trapping, depending solely on device geometry and device-particle surface interaction. The study reveals crucial parameters affecting device efficiency, particle trapping stiffness, and particle residence time, providing guidelines for future developments in optimization of device geometry. Various simulations, including charged particle and point charge approximation, are presented to estimate particle trapping efficiency, with good agreement with experimental observations.
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
(2021)
Review
Cell Biology
Joanna Kalita, Larisa E. Kapinos, Roderick Y. H. Lim
Summary: This review examines the role of the nuclear pore complex in nucleocytoplasmic transport and discusses unresolved issues such as the partitioning of importin and exportin in the cytoplasm and nucleus, and the maintenance of the RanGTP-RanGDP gradient across the nuclear envelope.
JOURNAL OF CELL SCIENCE
(2021)
Review
Physics, Multidisciplinary
Bart W. Hoogenboom, Loren E. Hough, Edward A. Lemke, Roderick Y. H. Lim, Patrick R. Onck, Anton Zilman
Summary: Eukaryotic cells are characterized by a nucleus containing the genome, surrounded by a nuclear envelope. They have evolved a molecular nanomachine called the Nuclear Pore Complex (NPC) to facilitate bi-directional transport between the cell nucleus and cytoplasm. The NPC combines high molecular specificity with high throughput and speed, and is robust to molecular noise and structural perturbations.
PHYSICS REPORTS-REVIEW SECTION OF PHYSICS LETTERS
(2021)
Article
Nanoscience & Nanotechnology
Deepika Sharma, Roderick Y. H. Lim, Thomas Pfohl, Yasin Ekinci
Summary: The focus of this work is on the development of simple and effective nanofluidic devices for trapping single charged nanoparticles in an aqueous environment. By modifying the surface of the device to change its charge properties and reducing the surface modification time, the study demonstrates the successful enhancement in device performance.
MICROSYSTEMS & NANOENGINEERING
(2021)
Article
Biochemistry & Molecular Biology
Lisa Diez, Larisa E. Kapinos, Janine Hochmair, Sabrina Huebschmann, Alvaro Dominguez-Baquero, Amelie Vogt, Marija Rankovic, Markus Zweckstetter, Roderick Y. H. Lim, Susanne Wegmann
Summary: This study investigates the interactions between Tau and FG Nup, and how Tau phosphorylation and oligomerization affect these interactions. The results suggest that phosphorylation facilitates the accumulation of Tau with FG Nup, while oligomerization inhibits their interactions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Materials Science, Biomaterials
Shabnam Tarvirdipour, Michal Skowicki, Cora-Ann Schoenenberger, Larisa E. Kapinos, Roderick Y. H. Lim, Yaakov Benenson, Cornelia G. Palivan
Summary: The design of non-viral vectors that efficiently deliver genetic materials into cells, particularly to the nucleus, is still a major challenge. This study introduces a delivery platform based on self-assembled peptides, which can form micellar nanostructures with nuclear localization signal (NLS). These nanostructures can encapsulate various oligonucleotides and enhance cellular uptake and nuclear translocation. The platform shows potential for efficiently delivering genetic payloads and has implications in basic research and biomedicine.
BIOMATERIALS SCIENCE
(2022)