Article
Polymer Science
Rebeca Leu Alexa, Horia Iovu, Jana Ghitman, Andrada Serafim, Cristina Stavarache, Maria-Minodora Marin, Raluca Ianchis
Summary: The focus of this study was on developing suitable GelMA hydrogel inks for 3D printing in tissue engineering applications. By exploring different degrees of GelMA methacrylation, concentrations of GelMA, and concentrations of photoinitiator, the researchers were able to determine the optimal formulations for the printing process. The presence of methacryloyl groups, the isoelectric point, hydrophilicity, mechanical properties, and structural integrity of the GelMA-based inks and 3D-printed scaffolds were thoroughly characterized to ensure their suitability for biological applications.
Article
Biotechnology & Applied Microbiology
Dongyuan Luo, Boying Chen, Yu Chen
Summary: The currently available biomaterials for alveolar cleft repair have poor mechanical properties and biocompatibility. In this study, a 3D printed biomimetic scaffold was developed using a mixture of beta-tricalcium phosphate (beta-TCP) and bone marrow mesenchymal stem cells (BMSCs). The hybrid scaffold showed good biocompatibility and mechanical properties, and supported the attachment, proliferation, survival, differentiation, and osteogenic induction of BMSCs for alveolar cleft reconstruction.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Engineering, Biomedical
Dewei Qiu, Chuanliang Cao, Aruna Prasopthum, Zhenchang Sun, Shan Zhang, Hanwen Yang, Zhiyong Xu, Jun Tao, Fanrong Ai, Jing Yang
Summary: The study investigated the interfacial bonding formation and the role of immune cells in bone-scaffold material interfaces. Different levels of foreign body response (FBR) were elicited by two different 3D printed porous scaffolds, with polymer/hydroxyapatite composite scaffolds showing more intensive FBR compared to sintered hydroxyapatite scaffolds. The presence of FBR components appeared to inhibit osseointegration.
MATERIALS TODAY BIO
(2023)
Review
Polymer Science
Nurulhuda Arifin, Izman Sudin, Nor Hasrul Akhmal Ngadiman, Mohamad Shaiful Ashrul Ishak
Summary: This article reviews the potential of photo-polymerization 3D printing technique in tissue engineering scaffold fabrication. It also compares this technique with other scaffold fabrication methods. Various parameter settings affecting mechanical properties, biocompatibility, and porosity behavior are discussed in detail.
Article
Materials Science, Coatings & Films
Alejandra Rodriguez-Contreras, Diego Torres, Belal Rafik, Monica Ortiz-Hernandez, Maria Pau Ginebra, Jose A. Calero, Jose Maria Manero, Elisa Ruperez
Summary: In this study, a thermochemical treatment was used to produce silver-doped calcium titanate coatings on titanium surfaces, enhancing bioactivity and providing antibacterial properties. The release of Ag ions from the coatings showed strong antibacterial effects, preventing bacterial adhesion and proliferation. Additionally, nanostructured topography of the coating reduced bacterial adhesion and proliferation even without Ag, maintaining bioactivity of the implants.
SURFACE & COATINGS TECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Thiago Domingues Stocco, Mayara Cristina Moreira Silva, Marcus Alexandre Finzi Corat, Gabriely Goncalves Lima, Anderson Oliveira Lobo
Summary: This study presents a new biofabrication strategy for developing biomimetic meniscus scaffolds. The use of bioprinted cell-laden hydrogel reinforced by aligned nanofiber sheets improved the structural integrity, shape fidelity, and mechanical properties of the scaffolds. Importantly, the introduction of nanofiber mats did not negatively affect cell viability. This bioengineering approach offers a promising strategy for tissue engineering of the knee meniscus.
INTERNATIONAL JOURNAL OF NANOMEDICINE
(2022)
Article
Pharmacology & Pharmacy
Fei Xing, Hua-Mo Yin, Man Zhe, Ji-Chang Xie, Xin Duan, Jia-Zhuang Xu, Zhou Xiang, Zhong-Ming Li
Summary: This study demonstrates the fabrication of a 3D-printed PCL scaffold with a nanotopographical surface, loaded with urine-derived stem cells (USCs) for bone regeneration. The nanotopographical 3D-printed scaffold shows enhanced protein adsorption and mineralization ability, promotes the growth and gene expression of USCs, and exhibits good bone regeneration capability in cranial bone defects.
Article
Cell Biology
Ui-Lyong Lee, Seokhwan Yun, Hua-Lian Cao, Geunseon Ahn, Jin-Hyung Shim, Su-Heon Woo, Pill-Hoon Choung
Summary: The study shows that hybrid artificial organs manufactured using 3D printing technology can create structures similar to periodontal ligaments around implants, thereby increasing the amount of connective tissue.
Article
Engineering, Multidisciplinary
Cuidi Li, Zhenjiang Ma, Wentao Li, Tianyang Jie, Liping Zhong, Hongfang Chen, Wenhao Wang, Jinwu Wang, Wenguo Cui, Yongxiang Zhao
Summary: The study combines self-assembled vaccines with 3D-printed scaffolds to activate local antigen-specific immune responses. The results show that this method effectively recruits and activates immune cells, promoting angiogenesis and bone regeneration.
Article
Engineering, Biomedical
Ashley L. Farris, Dennis Lambrechts, Yuxiao Zhou, Nicholas Y. Zhang, Naboneeta Sarkar, Megan C. Moorer, Alexandra N. Rindone, Ethan L. Nyberg, Alexander Perdomo-Pantoja, S. J. Burris, Kendall Free, Timothy F. Witham, Ryan C. Riddle, Warren L. Grayson
Summary: Oxygen delivery plays a crucial role in enhancing the therapeutic efficacy of cell transplants. In this study, researchers developed a novel scaffold material that effectively releases oxygen, leading to improved bone tissue regeneration mediated by adipose-derived stem cells.
Article
Neurosciences
Sara Simorgh, Rafieh Alizadeh, Ronk Shabani, Fariba Karimzadeh, Elham Seidkhani, Jamal Majidpoor, Fatemeh Moradi, Hamidreza Kasbiyan
Summary: Delivery of olfactory ectomesenchymal stem cells through intranasal route shows promising results in improving behavioral functions in the animal models of Parkinson's disease.
NEUROTOXICITY RESEARCH
(2021)
Article
Chemistry, Multidisciplinary
Shun Lu, Xiaocheng Wang, Wenzhao Li, Yan Zu, Jian Xiao
Summary: Stem-cell-based therapeutic strategies have shown promise in treating intrauterine adhesions (IUAs). However, endometrial regeneration still faces challenges. In this study, a customizable injectable porous hydrogel scaffold was created using a microfluidic-based 3D printing technique for delivering adipose-derived stem cells (ADSCs) to enhance endometrial regeneration. The scaffold supported cell encapsulation and maintained cell viability post-injection, leading to improved cell migration, proliferation, and tube formation. In rat models of IUAs, the ADSCs-laden scaffold promoted functional endometrial regeneration by suppressing inflammation, enhancing cell proliferation, and improving vascularization. This research highlights the potential of 3D-printed porous scaffolds as minimally invasive and effective strategies for endometrial regeneration.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Cell Biology
Rung-Shu Chen, Sheng-Hao Hsu, Hao-Hueng Chang, Min-Huey Chen
Summary: The study aimed to explore the feasibility of using adult dental pulp stem cells on polylactic acid scaffolds for tooth regeneration through experiments on dogs. The mineralization degree in the experimental group with cells seeded in the scaffolds was significantly higher than the control group, indicating the importance of dental pulp stem cells for tooth regeneration. However, there was incomplete absorption of the HA/PLA scaffolds in both groups, suggesting the need for a more rapidly absorbable material in scaffold design.
Article
Biochemistry & Molecular Biology
Emanuela Muscolino, Anna Barbara Di Stefano, Marco Trapani, Maria Antonietta Sabatino, Daniela Giacomazza, Sabina Alessi, Emanuele Cammarata, Francesco Moschella, Adriana Cordova, Francesca Toia, Clelia Dispenza
Summary: 3D printing of polymeric scaffolds and autologous stem cells using suitable bioinks is a promising approach for damaged facial cartilage reconstruction. In this study, hydrogel bioinks were developed and evaluated for their physical and chemical properties as well as their effects on stem cells. The results showed that viable cells can be successfully printed and cultured using the optimized process parameters.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Engineering, Biomedical
Huijuan Fu, Dequan Zhang, Jinshi Zeng, Qiang Fu, Zhaoyang Chen, Xuer Sun, Yi Yang, Shiyi Li, Minliang Chen
Summary: This study aimed to explore the in vivo application of a 3D-printed tissue-engineered skin substitute loaded with human adipose-derived stem cells (hADSCs) in wound healing. The newly designed biomaterial, composed of adipose tissue decellularized extracellular matrix (dECM) pre-gel, methacrylated gelatin (GelMA), and methacrylated hyaluronic acid (HAMA), was fabricated by 3D printing. The results showed that the 3D-printed skin substitute accelerated wound healing, promoting blood perfusion, re-epithelialization, collagen deposition, and angiogenesis.
INTERNATIONAL JOURNAL OF BIOPRINTING
(2023)
Article
Cell Biology
Fan Zhang, Shokouh Attarilar, Kegong Xie, Chao Han, Qingyang Liang, Ke Huang, Changgong Lan, Chong Wang, Chengliang Yang, Liqiang Wang, Masoud Mozafari, Kai Li, Jia Liu, Yujin Tang
Summary: The study indicated that PSME1/2 accumulation in OP affects bone metabolism, and CFZ inhibits PSME1 and PSME2 proteasome activities to promote osteogenesis and inhibit osteoclastogenesis by activating Wnt/beta-catenin signaling, suggesting a potential therapeutic target for postmenopausal OP.
MOLECULAR AND CELLULAR ENDOCRINOLOGY
(2022)
Correction
Chemistry, Multidisciplinary
Narendra Pal Singh Chauhan, Masoud Mozafari, Narendra Singh Chundawat, Kiran Meghwal, Rakshit Ameta, Suresh C. Ameta
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2023)
Article
Oncology
Ali Sadeghi, S. M. Hossein Naghavi, Masoud Mozafari, Ehsan Afshari
Summary: Terahertz (THz) technology is a non-invasive imaging system that shows great potential for biosensing and clinical diagnosis. It is particularly effective in detecting changes in water content and structural variations in diseased cells, making it useful for early detection of pathologic tissue, especially in oncology. However, THz imaging has limited contrast in living tissue for in-vivo clinical imaging. Nanotechnology has emerged as a solution to enhance the accuracy and specificity of THz images by using nanoparticles as contrast agents.
TRANSLATIONAL ONCOLOGY
(2023)
Article
Instruments & Instrumentation
Naser Amini, Ahmad Hivechi, Shiva Asadpour, Kaveh Ebrahimzadeh, Saeid Kargozar, Mazaher Gholipourmalekabadi, Ahvan Nasrolahi, Melina Ghasemian, Amir Shafaat, Masoud Mozafari, Peiman Brouki Milan, Alireza Rezapour
Summary: This study investigated the use of a novel biomimetic hybrid scaffold for skin regeneration, which continuously delivered stromal cell-derived factor-1 alpha (SDF-1 alpha). The scaffold demonstrated biocompatibility and its release of SDF-1 alpha was assessed. In diabetic rat models, the scaffold with SDF-1 alpha showed a significantly enhanced ability for wound regeneration and recruitment of CD31 and CXCR4-positive cells. The study concluded that the hybrid scaffold based on natural polymer offers a new option for topical administration of bioactive molecules and holds promise for skin tissue engineering.
DRUG DELIVERY AND TRANSLATIONAL RESEARCH
(2023)
Article
Materials Science, Multidisciplinary
Shukufe Amukarimi, Iman Mobasherpour, Sorosh Abdollahi, Peiman Brouki Milan, Masoud Mozafari
Summary: Thanks to prophylactic antibiotics, the risk of wound infection in orthopedic surgeries has significantly decreased. This study focuses on the effectiveness of a new biodegradable antibiotic-loaded coating on the surface of a magnesium implant. The results show that the coating is biocompatible, antibacterial, and has improved corrosion resistance, making it potentially suitable for bone regeneration applications.
MATERIALS CHEMISTRY AND PHYSICS
(2023)
Review
Biotechnology & Applied Microbiology
Hanieh Shokrani, Amirhossein Shokrani, Farzad Seidi, Mohammad Mashayekhi, Saptarshi Kar, Dragutin Nedeljkovic, Tairong Kuang, Mohammad Reza Saeb, Masoud Mozafari
Summary: 3D printing is an advanced technology used to fabricate biomaterials for translational medicine. With the introduction of stimuli-responsive properties, known as 4D printing, intelligent biomaterials with time-dependent characteristics have been developed. Polysaccharides, which are sensitive to various stimuli, are suitable for 3D and 4D printing. However, the mechanical strength and printability of polysaccharide-based scaffolds, as well as their aneural, avascular, and poor metabolic characteristics, need to be optimized by varying printing parameters. Multiple disciplines need to be integrated to achieve multipurpose printable biomaterials.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Article
Chemistry, Multidisciplinary
N. Alasvand, A. Behnamghader, P. B. Milan, M. Mozafari
Summary: This study successfully synthesized modified borate-based bioactive glasses with anticoagulant, antibacterial, and angiogenesis properties, showing great potential in blood-contact applications and vascular tissue engineering.
MATERIALS TODAY CHEMISTRY
(2023)
Article
Engineering, Biomedical
Neda Alasvand, Aliasghar Behnamghader, Peiman B. Milan, Sara Simorgh, Ali Mobasheri, Masoud Mozafari
Summary: This study aims to increase the success rate of implantation by fabricating bilayer vascular grafts containing bioactive glasses (BGs) and modifying their composition. The results demonstrated that the incorporation of modified BGs into the polymeric matrix not only improved the mechanical properties of the vascular graft but also significantly enhanced its antibacterial activity against both gram-negative and gram-positive strains.
MATERIALS TODAY BIO
(2023)
Correction
Nanoscience & Nanotechnology
Navid Rabiee, Mojtaba Bagherzadeh, Amir Mohammad Ghadiri, Yousef Fatahi, Abdullah Aldhaher, Pooyan Makvandi, Rassoul Dinarvand, Maryam Jouyandeh, Mohammad Reza Saeb, Masoud Mozafari, Mohammadreza Shokouhimehr, Michael R. Hamblin, Rajender S. Varma
ACS APPLIED BIO MATERIALS
(2023)
Article
Engineering, Biomedical
Ali Sadeghi, Ehsan Afshari, Mohadeseh Hashemi, David Kaplan, Masoud Mozafari
Summary: Neural interfaces (NIs) are neuroengineering tools that enable a mutual communication pathway with the nervous system. Smart biomaterials (SBMs) such as stimuli-responsive and shape memory biomaterials emerged as novel materials with exceptional properties, enhancing the function of NIs and opening new possibilities in neuroscience studies and neuromodulation therapies.
CURRENT OPINION IN BIOMEDICAL ENGINEERING
(2023)
Article
Engineering, Biomedical
Mir Saman Safavi, Aydin Bordbar-Khiabani, Frank C. Walsh, Masoud Mozafari, Jafar Khalil-Allafi
Summary: NiTi metallic biomaterials have a wide range of clinical applications, and the use of NiTi smart biomaterials has gained attention for their unique features. Surface-modification techniques, including dry and wet coating techniques, have been employed to improve the biological properties of NiTi before clinical use. R&D activities have shown progress, but more work needs to be done before coated-NiTi can be used clinically.
CURRENT OPINION IN BIOMEDICAL ENGINEERING
(2023)
Article
Oncology
Ali Salehi, Parvaneh Naserzadeh, Parastoo Tarighi, Elham Afjeh-Dana, Masoud Akhshik, Amir Jafari, Pooyan Mackvandi, Behnaz Ashtari, Masoud Mozafari
Summary: Breast cancer is a prevalent cancer in women, and microfluidic devices show promise in advancing research in this field. This study used a dynamic cell culture condition in a microfluidic concentration gradient device to assess the anticancer activities of probiotic strains against breast cancer cells. The findings revealed that a specific concentration of probiotic supernatant induced more cell death signaling after 48 hours. Additionally, the evaluated optimum dose was lower than the conventional treatment dose. Flow cytometric assessment confirmed that cell death signaling was concentration and time dependent. This study demonstrates the potential benefits of microfluidic platforms in personalized medicine and cancer therapy.
TRANSLATIONAL ONCOLOGY
(2023)
Article
Oncology
Przemys law Gnatowski, Edyta Pilat, Justyna Kucinska-Lipka, Mohammad Reza Saeb, Michael R. Hamblin, Masoud Mozafari
Summary: Cancerous tumors are highly fatal, claiming almost 10 million lives in 2020 worldwide. Accurately modeling tumors is a challenging task due to their complex and dynamic nature. Hydrogel-based 3D bioprinting is emerging as a promising approach to overcome the limitations of current models and mimic cancer development and behavior.
TRANSLATIONAL ONCOLOGY
(2023)
Review
Materials Science, Biomaterials
Fatemeh Kabirian, Masoud Mozafari, Petra Mela, Ruth Heying
Summary: The use of 3D printing for biodegradable implants opens up new possibilities in cardiovascular research. This method allows for the design and fabrication of complex structures based on patient imaging data in an accurate, reproducible, cost-effective, and quick manner. Biodegradable cardiovascular implants have the potential to provide support for tissue regeneration while preventing long-term complications.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Review
Biochemistry & Molecular Biology
Maziar Malekzadeh Kebria, Peiman Brouki Milan, Noshad Peyravian, Jafar Kiani, Soheil Khatibi, Masoud Mozafari
Summary: Treating post-COVID complications has been a challenge, but stem cell therapy shows promise in modulating the immune system and providing long-term protection.
MOLECULAR BIOMEDICINE
(2022)