A critical analysis of laboratory and clinical research methods to study root and canal anatomy

The technical execution of root canal treatment procedures demands a thorough understanding and knowledge of root and canal anatomy. Over the decades, and with the aid of various research methods such as staining and clearing, 2D radiographic imaging, sectioning procedures, cone beam computed tomography and micro-computed tomography, many laboratory and clinical studies have been undertaken to understand the root and canal anatomy of the human dentition. This has resulted in a tremendous increase in the body of knowledge with a wide range of qualitative and quantitative presentations of the root and canal anatomy. This review aims to provide a critical analysis for the laboratory and clinical research methods in root and canal anatomy studies. In addition, it aims to identify existing gaps and present insights for directions of future research and ways for translation to clinical endodontics.

Automated summary

The technical execution of root canal treatment procedures requires a thorough understanding of root and canal anatomy. Various research methods have been employed to study the root and canal anatomy of human dentition, resulting in an increased body of knowledge. This review critically analyzes laboratory and clinical research methods and suggests future research directions and translation to clinical endodontics.