Journal
EXPERIMENTAL AND MOLECULAR PATHOLOGY
Volume 124, Issue -, Pages -Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.yexmp.2021.104738
Keywords
Esophageal squamous cell carcinoma; miR-335-5p; Cell proliferation; TTK; Luciferase reporter assay
Categories
Funding
- Science and Engineering Research Board (SERB) Department of Science and Technology, Ministry of Science and Technology, Government of India [PDF/2015/001015]
- Kiran Division, Department of Science and Technology, Ministry of Science and Technology, Government of India [SR/WOS-A/LS-309/2018(G)]
- Council of Scientific and Industrial Research, Government of India [27(0342)/19/EMR-II]
- Indian Council of Medical Research, Government of India [2019-0940/GEN/ADHOC/BMS]
- Guru Gobind Singh Indraprastha University (GGSIPU) [GGSIPU/DRC/FRGS/2018/19(1115)]
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The research revealed that miR-335-5p plays a tumor suppressive role in esophageal cancer by inhibiting cell proliferation, migration, and invasion. Through RNA-seq and Insilico analysis, TTK was identified as a newly discovered direct target of miR-335-5p, providing insights into its potential role in esophageal tumorigenesis.
Background: Esophageal cancer is an aggressive malignancy. miR-335-5p is reported to possess both tumour suppressor and tumour promoter activities in different cancers. Objectives: We investigated the role of miR-335-5p in esophageal cancer by expression and functional studies. Materials and methods: The role of miR-335-5p in ESCC was evaluated using MTT assay, cell cycle analysis, colony formation assay, scratch assay, matrigel invasion, and migration assay. Results: Our expression studies showed a significantly decreased expression of tissue and circulating miR-335-5p in esophageal cancer. Our results herein report a key tumour suppressive role of miR-335-5p in esophageal carcinogenesis by inhibiting proliferation, migration, and invasion in ESCC cells. Using RNA-seq and Insilico analysis we found TTK to be a newly identified direct target and confirmed it by using luciferase assay. Conclusion: Overall, our expression and functional analysis results demonstrated herein point towards the potential role of miR-335-5p in esophageal tumorigenesis. Moreover, this is the first report showing TTK as a downstream target of miR-335-5p.
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