Review
Physiology
Jea Giezl Niedo Solidum, Youngjae Jeong, Francisco Heralde III, Dongsu Park
Summary: Skeletal stem/progenitor cells (SSPCs) play crucial roles in skeletal development, bone remodeling, and repair. Recent studies have revealed the presence of distinct SSPC populations in various skeletal compartments. The regulatory mechanisms for these cells differ based on their cellular and matrix environment, embryonic origin, and adjacent tissue structures. Understanding these regulatory differences is important for discovering novel treatment approaches for bone repair.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Endocrinology & Metabolism
Anais Julien, Simon Perrin, Ester Martinez-Sarra, Anuya Kanagalingam, Caroline Carvalho, Marine Luka, Mickael Menager, Celine Colnot
Summary: Bone regeneration involves the activation of skeletal stem/progenitor cells (SSPCs) from bone marrow, periosteum, and skeletal muscle. The periosteum and skeletal muscle-derived SSPCs are enriched in osteochondral progenitors and have a more efficient contribution to endochondral ossification during fracture repair compared to bone marrow-derived stromal cells. Activation of SSPCs in periosteum and skeletal muscle is mediated by bone morphogenetic protein (BMP) signaling, and this coordinated molecular program is essential for bone healing and differentiation.
JOURNAL OF BONE AND MINERAL RESEARCH
(2022)
Article
Cell & Tissue Engineering
Christopher L. Haga, Cori N. Booker, Ana Carvalho, Siddaraju Boregowda, Donald G. Phinney
Summary: Despite clinical testing, MSC-based therapies are not effective due to lack of predictive biomarkers. TWIST1 predicts inter-donor differences in MSC quality attributes. The present work identified TWIST1 targets related to ECM structure, skeletal and circulatory system development, interferon gamma signaling, and inflammation, which indicates the mechanistic link between stem/progenitor and paracrine activities of MSCs. The findings provide potential genes for further studies.
Article
Biochemistry & Molecular Biology
Gregor Haring, Janja Zupan
Summary: This study aimed to identify the optimal tissue source for the isolation of primary cells with mesenchymal stem/stromal cell (MSC)-like properties and to determine the influence of post mortem time on these cells. The results showed that knee and peri-knee tissues from donors even 3 days post mortem are strategic sources of MSCs for regenerative procedures.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Genetics & Heredity
Shuanglong Lu, Xiaohong Qiao
Summary: This study compared the differences in cell cycle, stemness, differentiative potencies, and immunomodulate capability between unlicensed and licensed human bone marrow-derived MSCs using single-cell RNA sequencing data. The results showed that licensed MSCs exhibited enhanced immunomodulate capability and altered differentiation potencies compared to unlicensed MSCs.
Article
Biology
Jason T. Long, Abigail Leinroth, Yihan Liao, Yinshi Ren, Anthony J. Mirando, Tuyet Nguyen, Wendi Guo, Deepika Sharma, Douglas Rouse, Colleen Wu, Kathryn Song Eng Cheah, Courtney M. Karner, Matthew J. Hilton
Summary: The study reveals that hypertrophic chondrocytes undergo dedifferentiation to give rise to marrow-associated skeletal stem and progenitor cells (SSPCs), which serve as a primary source of osteoblasts during skeletal development and also contribute to the adipogenic lineage.
Article
Endocrinology & Metabolism
Alexander Vesprey, Eun Sung Suh, Didem Goz Ayturk, Xu Yang, Miracle Rogers, Branden Sosa, Yingzhen Niu, Ivo Kalajzic, Lionel B. Ivashkiv, Mathias P. G. Bostrom, Ugur M. Ayturk
Summary: This study identified specific bone marrow stromal cell populations essential for bone formation around metal implants. P alpha S cells are mobilized after surgery to repair bone tissue and participate in implant osseointegration.
JOURNAL OF BONE AND MINERAL RESEARCH
(2021)
Review
Cell Biology
Qianmin Gao, Lipeng Wang, Sicheng Wang, Biaotong Huang, Yingying Jing, Jiacan Su
Summary: Bone marrow mesenchymal stromal cells are a group of heterogeneous cells consisting of multipotent stem cells, including osteochondral and adipocyte progenitors. Recent studies have identified new subgroups of BMSCs and clarified their roles in normal and pathological conditions. Furthermore, key regulators and mechanisms controlling the fate of BMSCs have been revealed.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Samantha Donsante, Biagio Palmisano, Marta Serafini, Pamela G. Robey, Alessandro Corsi, Mara Riminucci
Summary: Bone formation begins in the embryonic stage and continues throughout life, involving stages of bone modeling and growth, remodeling, and regeneration. Osteoblasts produce, assemble, and control mineralization of the bone matrix, while regulating other cell processes and metabolic activities. Osteoblasts are derived from different skeletal stem cells under the control of multiple regulators at different developmental stages.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Maria Xydia, Raheleh Rahbari, Eliana Ruggiero, Iain Macaulay, Maxime Tarabichi, Robert Lohmayer, Stefan Wilkening, Tillmann Michels, Daniel Brown, Sebastiaan Vanuytven, Svetlana Mastitskaya, Sean Laidlaw, Niels Grabe, Maria Pritsch, Raffaele Fronza, Klaus Hexel, Steffen Schmitt, Michael Mueller-Steinhardt, Niels Halama, Christoph Domschke, Manfred Schmidt, Christof von Kalle, Florian Schuetz, Thierry Voet, Philipp Beckhove
Summary: Regulatory T cells in breast cancer do not show clonal relationship with their circulating counterpart, but share a common origin with intratumoral antigen-experienced conventional T cells.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Sixun Wu, Shinsuke Ohba, Yuki Matsushita
Summary: The bone marrow is a complex mixture of hematopoietic, vascular, and skeletal cells, with skeletal stem and progenitor cells (SSPCs) located upstream of the hierarchy. These cells differentiate into various bone and marrow cells, and their potential shift may occur with age. Alterations in cell composition contribute to bone regeneration and aging-related bone diseases. In this review, the cellular dynamics of skeletal cell populations in bone homeostasis, regeneration, and osteoporosis are discussed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Brielle Jones, Chaoyang Li, Min Sung Park, Anne Lerch, Vimal Jacob, Nicholas Johnson, Jin-Qiang Kuang, Sandeep Dhall, Malathi Sathyamoorthy
Summary: The study compared the gene expression differences between amniotic stromal cells (ASCs) and chorionic stromal cells (CSCs), revealing that CSCs have superior angiogenic potential while ASCs exhibit increased anti-inflammatory properties.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Nian Zhang, Liru Hu, Zhiwei Cao, Xian Liu, Jian Pan
Summary: Bone has remarkable ability to self-repair without leaving scars, thanks to tissue-resident stem cells known as skeletal stem cells (SSCs). While bone marrow SSCs have been extensively studied, our understanding of periosteal SSCs (P-SSCs) is limited. Researchers have recently developed strategies to successfully identify P-SSCs and provide visual evidence of their stemness. P-SSCs and BM-SSCs share similarities but also have distinct properties. This review provides an updated overview of P-SSCs and their specific responses to bone injury.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Stefania Crippa, Anastasia Conti, Valentina Vavassori, Samuele Ferrari, Stefano Beretta, Silvia Rivis, Roberto Bosotti, Serena Scala, Stefania Pirroni, Raisa Jofra-Hernandez, Ludovica Santi, Luca Basso-Ricci, Ivan Merelli, Pietro Genovese, Alessandro Aiuti, Luigi Naldini, Raffaella Di Micco, Maria Ester Bernardo
Summary: Mesenchymal stromal cells (MSCs) have been shown to play a supportive role in the expansion and engraftment of hematopoietic stem and progenitor cells (HSPCs). In this study, we developed a co-culture system using bone marrow (BM)-MSCs to improve the transplantation outcome of CRISPR-Cas9 gene-edited (GE) human HSPCs. We found that BM-MSCs can produce hematopoietic supportive and anti-inflammatory factors that enhance the expansion and clonogenic potential of GE-HSPCs in vitro, leading to improved engraftment and early phase hematological reconstitution in transplanted mice. This study suggests a potential clinical application of BM-MSCs in promoting successful transplantation of GE-HSPCs.
Review
Endocrinology & Metabolism
Yuki Matsushita, Wanida Ono, Noriaki Ono
Summary: Bone marrow contains precursor cells for osteoblasts and adipocytes. Recent studies have identified subsets of bone marrow stromal cells (BMSCs) that can differentiate into marrow adipocytes and possess diverse functions in homeostasis and regeneration. The trajectory of BMSCs towards marrow adipocytes is influenced by multiple modes of cell-autonomous and non-cell autonomous regulations.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Biochemical Research Methods
Xiao Huo, Sheng'en Hu, Chengchen Zhao, Yong Zhang
Article
Biology
Yixuan Wang, Chengchen Zhao, Zhenzhen Hou, Yuanyuan Yang, Yan Bi, Hong Wang, Yong Zhang, Shaorong Gao
Article
Biochemistry & Molecular Biology
Qixuan Wang, Jinyan Huang, Hanfei Sun, Jing Liu, Juan Wang, Qian Wang, Qian Qin, Shenglin Mei, Chengchen Zhao, Xiaoqin Yang, X. Shirley Liu, Yong Zhang
NUCLEIC ACIDS RESEARCH
(2014)
Article
Multidisciplinary Sciences
Sheng'en Hu, Xiaolan Chen, Ji Liao, Yiqing Chen, Chengchen Zhao, Yong Zhang
Article
Multidisciplinary Sciences
Chengchen Zhao, Sheng'en Hu, Xiao Huo, Yong Zhang
Article
Cell Biology
Yan Bi, Zhifen Tu, Yanping Zhang, Peng Yang, Mingyue Guo, Xuehao Zhu, Chengchen Zhao, Jianfeng Zhou, Hong Wang, Yixuan Wang, Shaorong Gao
Article
Biochemical Research Methods
Chengchen Zhao, Wenchao Xiu, Yuwei Hua, Naiqian Zhang, Yong Zhang
Summary: CStreet is a computed Cell State trajectory inference method for time-series scRNA-seq data that constructs k-nearest neighbor connections between cells and estimates the connection probabilities, visualizing the trajectory. Compared to other methods, CStreet demonstrates high accuracy and tolerance.
Article
Biochemical Research Methods
Shiyang Zeng, Yuwei Hua, Yong Zhang, Guifen Liu, Chengchen Zhao
Summary: GLEANER is a web server dedicated to the analysis and visualization of multiomics data related to the mammalian germline cycle, providing a comprehensive collection of transcriptome, DNA methylome, chromatin accessibility, and histone modification characteristics. It displays integrative analysis results for each input gene, genes with correlated transcriptional changes, and overall continuous dynamic trends in the germline cycle.
BMC BIOINFORMATICS
(2021)
Article
Multidisciplinary Sciences
Bo Wang, Chen Li, Jin Ming, Linlin Wu, Shicai Fang, Yi Huang, Lihui Lin, He Liu, Junqi Kuang, Chengchen Zhao, Xingnan Huang, Huijian Feng, Jing Guo, Xuejie Yang, Liman Guo, Xiaofei Zhang, Jiekai Chen, Jing Liu, Ping Zhu, Duanqing Pei
Summary: Cell fate decision involves rewiring of the genome, but remains poorly understood at the chromatin level. Chromatin remodeling complex NuRD participates in closing open chromatin in the early phase of reprogramming. The Sall4-NuRD axis plays a critical role in closing the open chromatin in the early phase of reprogramming.
NATURE COMMUNICATIONS
(2023)
Article
Biotechnology & Applied Microbiology
Chengchen Zhao, Naiqian Zhang, Yalin Zhang, Nuermaimaiti Tuersunjiang, Shaorong Gao, Wenqiang Liu, Yong Zhang