Immune landscape of human placental villi using single-cell analysis

Maintenance of a healthy pregnancy is reliant on a successful balance between the fetal and maternal immune systems. Although the maternal mechanisms responsible have been well studied, those used by the fetal immune system remain poorly understood. Using suspension mass cytometry and various imaging modalities, we report a complex immune system within the mid-gestation (17-23 weeks) human placental villi (PV). Consistent with recent reports in other fetal organs, T cells with memory phenotypes, although rare in abundance, were detected within the PV tissue and vasculature. Moreover, we determined that T cells isolated from PV samples may be more proliferative after T cell receptor stimulation than adult T cells at baseline. Collectively, we identified multiple subtypes of fetal immune cells within the PV and specifically highlight the enhanced proliferative capacity of fetal PV T cells.

Automated summary

Maintenance of a healthy pregnancy depends on the balance between the fetal and maternal immune systems. This study used advanced techniques to investigate the immune system in mid-gestation human placental villi and found multiple subtypes of fetal immune cells, including memory T cells. It was also discovered that T cells isolated from placental villi had enhanced proliferative capacity compared to adult T cells.