Time-resolved X-ray scattering studies of proteins

Time-resolved small- and wide-angle X-ray scattering studies of proteins in solution based on the pump-probe approach unveil structural information from intermediates over a broad range of length and time scales. In spite of the promise of this methodology, only a fraction of the wealth of information encoded in scattering data has been extracted in studies performed thus far. Here, we discuss the methodology, summarize results from recent time-resolved X-ray scattering studies, and examine the potential to extract additional information from these scattering curves.

Automated summary

Time-resolved small- and wide-angle X-ray scattering studies of proteins in solution using the pump-probe approach can provide structural information from intermediates across different length and time scales. While promising, only a fraction of the potential information encoded in scattering data has been extracted so far. The article discusses the methodology, summarizes recent results, and explores the potential for extracting additional information from these scattering curves.