Journal
COMPOSITES PART B-ENGINEERING
Volume 226, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.compositesb.2021.109364
Keywords
Glucose starvation therapy; Phototherapy; Chemodynamic therapy; Enzymatic cascade reaction; Multifunctional nanocomposites
Funding
- Ministry of Science and Technology of the Republic of China [MOST 108-2218-E-011-017-MY3, 110-2113-M-011-003]
- Taiwan Building Technology Center from The Featured Areas Research Center Program by the Ministry of Education in Taiwan
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This study demonstrates the regulation of the reactive oxygen species (ROS) scavenging or generation ability of carbon dots (CDs) by incorporating heteroatoms, leading to the creation of novel nanocomposites with efficient therapeutic effects in both photodynamic therapy and chemodynamic therapy. The multifunctional nanocomposites show satisfactory photothermal conversion efficiencies, high ROS yield, and good biocompatibility, offering potential for enhanced cancer therapy through a combination of different treatment modalities.
In this study, the reactive oxygen species (ROS) scavenging or generation ability of the carbon dots (CDs) was regulated by incorporating with heteroatoms (Cu and Cl ions). The pristine CDs were found to be powerful anti-oxidants to scavenge ROS, with half-maximal inhibitory concentrations (IC50) of center dot O-2(-) and center dot OH radicals estimated to be 6.89 and 6.12 mu g/mL, respectively, whereas Cu and Cl co-doped CDs (CuCl-CDs) possessed not only ROS generation ability upon laser irradiation for photodynamic therapy (PDT), but also peroxidase-mimic activity that generates oxidative center dot OH from hydrogen peroxide (H2O2) for chemodynamic therapy (CDT). Moreover, the colorimetric assay, O-1(2) emission peak, and ESR results supported the efficient production of center dot O-2(-), center dot OH, and O-1(2) radicals. Furthermore, CuCl-CDs with ROS-generating abilities and peroxidase-mimetic properties were successfully integrated with polydopamine (PDA) and glucose oxidase (GOx) to fabricate multifunctional GOx/CuCl-CD@PDA-PEG (GCP) nanocomposites with hydrodynamic sizes of 135.5 nm. These novel GCP nanocomposites possessed satisfactory photothermal conversion efficacies (eta = 24.4%) and gave a high yield of ROS via the combination of H2O2 and laser irradiation. Moreover, the presence of GOx in GCP nanocomposites enables these compounds to decrease the intracellular glucose levels for starvation therapy and the enzymatic cascade activity for enhanced ROS-mediated therapy. In vitro studies and confirmed that these GCP nanocomposites displayed good biocompatibility with concentrations from 100 to 1000 ppm, but induced 90% reduction in B16F1 cell viability at 200 ppm via the cooperative effects of CDT, phototherapeutic effect, and starvation therapy.
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