Review
Chemistry, Multidisciplinary
Yi Xie, Huimin Li, Fumin Chen, Srisruthi Udayakumar, Khyati Arora, Hui Chen, Yang Lan, Qinqin Hu, Xiaonong Zhou, Xiaokui Guo, Leshan Xiu, Kun Yin
Summary: Mitigating the spread of global infectious diseases requires rapid and accurate diagnostic tools. This review highlights the recent advances in CRISPR-based microfluidic systems for infectious diseases diagnosis, including detection mechanisms, microfluidic devices, and improvement strategies.
Review
Behavioral Sciences
Arshad Mehmood, Wajid Ali, Zaheer Ud Din, Shuang Song, Muhammad Sohail, Wahid Shah, Jiangyuan Guo, Ruo-Yi Guo, Ikram Ilahi, Suleman Shah, Fadhl Al-Shaebi, Liaqat Zeb, Ernest Amponsah Asiamah, Zaid Al-Dhamin, Hazrat Bilal, Bin Li
Summary: Genome-editing technology such as CRISPR/Cas9 has bridged the translational gap in gene therapy treatments, particularly in the experimental and therapeutic applications for central nervous system diseases. Studies have identified genetic mutations and duplications related to Parkinson's disease pathogenesis, suggesting that gene editing through CRISPR technology could be beneficial for PD treatment. This highlights the importance and potential of CRISPR technology in research.
BRAIN AND BEHAVIOR
(2021)
Article
Veterinary Sciences
Hung-Yueh Yeh, Amal Awad, Michael J. Rothrock
Summary: The study explored the diversity of CRISPR sequences in C. jejuni isolates from an animal farm, revealing various combinations of CRISPR sequence lengths and spacer numbers. The results indicated that CRISPR genotyping combined with MLST methods could discriminate C. jejuni isolates, highlighting the need for further research on the application of CRISPR in outbreaks.
VETERINARY MEDICINE AND SCIENCE
(2021)
Article
Biotechnology & Applied Microbiology
Cheng Yang, Wangfeng Shao, Lingling Wei, Lingxiao Chen, Aihua Zhu, Zhiming Pan
Summary: Salmonella as a zoonotic pathogen, especially in transmission between household pets and humans, has attracted worldwide attention. A study conducted in Xuzhou, Jiangsu Province, China, investigated the distribution of dog Salmonella from pet hospitals and breeding base. The results showed that S. Kentucky was the most prevalent serotype, and CRISPR genes have better recognition ability for the same serotype.
Article
Biology
Marco Tatullo, Luisa Limongelli, Rosa maria Marano, Lessandra Valletta, Ngela Tempesta, Andro Rengo
Summary: The CRISPR-Cas9 system is a biotechnology that improves the efficacy of traditional treatments, particularly in the field of oral medicine. By speeding up and customizing therapies, this technology enhances the predictability of clinical outcomes and opens up new possibilities for treating oral diseases.
Review
Cell Biology
Manuel M. Vicente, Miguel Chaves-Ferreira, Joao M. P. Jorge, Joao T. Proenca, Vasco M. Barreto
Summary: The repurposing of the CRISPR/Cas bacterial defense system as molecular tools has revolutionized gene editing, and efforts have been made since 2013 to decrease the frequency of unwanted mutations during CRISPR-based gene editing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Plant Sciences
Yuchun Rao, Xi Yang, Chenyang Pan, Chun Wang, Kejian Wang
Summary: CRISPR-Cas9 is a promising gene editing technology with the advantages of simplicity, high efficiency, high specificity, and low cost. This review systematically summarizes its generation, advantages, and three derivative technologies, as well as its applications in crop improvement.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Genetics & Heredity
Hua Jiang, Kun Duan, Xu Han, Jun Wang, Xiao Liu, Maoxiao Yan, Yunxiu Wang, Hongyan Liu, Huiling Shi, Xiaoqing Gao, Chuan Ouyang, Xue Fu, Xinxin Zhang, Chao Liu
Summary: In this study, Cas13a was utilized for the detection of genetic mutations by isothermally amplifying mitochondrial point mutations from human genomic DNA. The approach involved the use of transcription-mediated amplification to generate CRISPR RNA, demonstrating the potential for rapid mutation detection within 90 minutes. Various designs of CRISPR DNA were explored to enhance assay specificity, with the possibility of a one-step single-tube reaction for mutation detection and the potential for automated applications in screening mitochondrial diseases.
FRONTIERS IN GENETICS
(2021)
Article
Chemistry, Multidisciplinary
Xianqing Wang, Yinghao Li, Xi Wang, Dario M. Sandoval, Zhonglei He, A. Sigen, Irene Lara Saez, Wenxin Wang
Summary: Protein therapeutics show great promise for treating complex diseases, but the lack of suitable delivery vectors limits their clinical use, particularly for delivering functional cytosolic proteins in vivo. This study demonstrates that modifying poly(ss amino ester)s (PAEs) with a phenyl guanidine (PG) group enhances their capabilities for intracellular delivery of cytosolic functional proteins and CRISPR/Cas9 ribonucleoprotein.
Article
Biotechnology & Applied Microbiology
Yang-min Wang, Hao-zheng Wang, Ying-zhen Jian, Zhong-tao Luo, Hong-wei Shao, Wen-feng Zhang
Summary: The CRISPR system is a highly valuable gene editing tool, but the induction of off-target effects during targeted editing remains a major challenge. Continuous improvement and optimization of the CRISPR system for reduction of off-target effects are crucial in CRISPR research.
HUMAN GENE THERAPY
(2022)
Article
Chemistry, Multidisciplinary
Yuzhi Chen, Zhi Chen, Tianzhong Li, Meng Qiu, Jinghan Zhang, Yan Wang, Wu Yuan, Aaron Ho-Pui Ho, Omar Al-Hartomy, Swelm Wageh, Abdullah G. Al-Sehemi, Xin Shi, Jingfeng Li, Zhongjian Xie, Li Xuejin, Han Zhang
Summary: This study presents a CRISPR/Cas12a-based diagnostic platform with a surface plasmon resonance-based fiber tip (CRISPR-SPR-FT) biosensor for detecting the mpox virus. The compact CRISPR-SPR-FT biosensor offers high stability and portability, and can analyze mpox viral DNA without amplification in under 1.5 hours with a limit of detection below 5 aM.
Article
Chemistry, Multidisciplinary
Huahua Yue, Mengqi Huang, Tian Tian, Erhu Xiong, Xiaoming Zhou
Summary: CRISPR-based diagnoses, derived from gene-editing technology, have unique features such as recognition at physiological temperature, excellent specificity, and high-efficiency signal amplification capabilities. Micro/nanotechnologies play key roles in the advancement of CRISPR diagnosis, including the use of nanoprobes, nanochips, microfluidic technology, and lateral flow strips, leading to new opportunities and improvements such as equipment-free detection, more compact detection systems, improved sensitivity, and quantitative capabilities. Despite significant progress, CRISPR diagnosis has not yet reached its full potential, with upcoming opportunities and improvements expected to be driven by micro/nanotechnologies.
Review
Biochemical Research Methods
Sara Selma, Evi Ceulemans, Alain Goossens, Elia Lacchini
Summary: The plant kingdom is a major source of feedstock, food, and added-value compounds. Engineering plant metabolic pathways is challenging due to complex interaction networks involving multiple genes, enzymatic steps, and metabolites. However, the development of CRISPR-Cas technologies has provided efficient tools for multigene editing in plants, enabling improvements in agronomic and metabolic traits. This article provides an overview of the achievements and perspectives of CRISPR technology in plant metabolic engineering.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jen-Hui Tsou, Hongjie Liu, Sanford A. Stass, Feng Jiang
Summary: This study developed a rapid CRISPR test for sensitive detection of SARS-CoV-2 without the need for RNA extraction, allowing for completion within one hour. The test is simple to operate, requiring minimal equipment, and results can be visualized immediately using a UV light illuminator or paper strips.
Review
Chemistry, Multidisciplinary
Mengdi Bao, Qun Chen, Zhiheng Xu, Erik C. Jensen, Changyue Liu, Jacob T. Waitkus, Xi Yuan, Qian He, Peiwu Qin, Ke Du
Summary: CRISPR, with its unique properties, has emerged as a powerful molecular biosensing tool for nucleic acids and biomarkers. However, several key challenges need to be addressed for it to become a new gold standard. This paper reviews the history of biosensors, discusses current challenges and recent breakthroughs in CRISPR-based nucleic acid detection, and focuses on future advancements needed for CRISPR-based molecular biosensors.
Review
Cell Biology
Glynnis A. Garry, Rhonda Bassel-Duby, Eric N. Olson
Summary: Ischemic heart disease is the leading cause of morbidity and mortality worldwide, and finding new ways to promote cardiomyocyte regeneration is crucial. Direct reprogramming of cardiac fibroblasts into induced cardiac-like myocytes (iCMs) has shown promising potential, but there are challenges in reprogramming adult human fibroblasts.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Catherine A. Makarewich, Amir Z. Munir, Svetlana Bezprozvannaya, Aaron M. Gibson, Soo Young Kim, Misty S. Martin-Sandoval, Thomas P. Mathews, Luke I. Szweda, Rhonda Bassel-Duby, Eric N. Olson
Summary: New evidence suggests that certain noncoding RNA molecules actually code for microproteins. By using comparative genomics, researchers identified a microprotein called Mtlbn that is abundantly expressed in the heart. Mtlbn localizes to the inner mitochondrial membrane and interacts with subunits of the electron transport chain and respiratory supercomplexes.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Cell Biology
Peiheng Gan, Zhaoning Wang, Maria Gabriela Morales, Yu Zhang, Rhonda Bassel-Duby, Ning Liu, Eric N. Olson
Summary: This study identified a genetic defect associated with noncompaction cardiomyopathy and revealed some underlying mechanisms. The loss of Rbpms gene led to premature cardiomyocyte binucleation and cell cycle arrest, in which Rbpms mediated the isoform switching of a specific protein.
DEVELOPMENTAL CELL
(2022)
Article
Medicine, Research & Experimental
Dileep R. Karri, Yu Zhang, Francesco Chemello, Yi-Li Min, Jian Huang, Jiwoong Kim, Pradeep P. A. Mammen, Lin Xu, Ning Liu, Rhonda Bassel-Duby, Eric N. Olson
Summary: This study demonstrates the long-term durability of CRISPR/Cas9 genome editing as a therapy for maintaining the integrity and function of DMD muscle, even under conditions of stress.
MOLECULAR THERAPY-NUCLEIC ACIDS
(2022)
Article
Cardiac & Cardiovascular Systems
Ning Liu, Eric N. Olson
Summary: Cardiovascular disease is the leading cause of deaths and illnesses in developed countries. Mouse models have played a significant role in studying the complex signaling pathways, genetic and epigenetic regulations, and multicellular interactions involved in cardiovascular disease. The advent of CRISPR genome editing has revolutionized cardiovascular research, allowing for rapid modeling and potential genetic correction of disease-causing mutations.
CIRCULATION RESEARCH
(2022)
Article
Immunology
Ildiko Bock-Marquette, Klaudia Maar, Szabolcs Maar, Balint Lippai, Gabor Faskerti, Ferenc Gallyas, Eric N. Olson, Deepak Srivastava
Summary: Our dream of defeating the processes of organ damage and aging remains a challenge, and steps towards regenerating individual organs are considered significant. Through interconnecting our collective knowledge regarding aging and embryonic development, we propose to discover molecules that can effectively reverse cellular damage. Our results regarding Thymosin beta-4 (TB4) support this hypothesis, showing its potential to promote cardiac cell migration and survival, enhance myocyte survival and improve cardiac function, and activate epicardial progenitor cells.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
Xurde M. Caravia, Andres Ramirez-Martinez, Peiheng Gan, John R. McAnally, Rhonda Bassel-Duby, Ning Liu, Eric N. Olson
CIRCULATION RESEARCH
(2022)
Article
Medicine, Research & Experimental
Xurde M. Caravia, Andres Ramirez-Martinez, Peiheng Gan, Feng Wang, John R. McAnally, Lin Xu, Rhonda Bassel-Duby, Ning Liu, Eric N. Olson
Summary: Mutations in nuclear envelope proteins cause devastating genetic diseases that primarily affect the heart and skeletal muscle. This study reveals the essentiality of LEMD2 for genome stability and cardiac function, and uncovers its mechanistic association with human disease. Gene therapy targeting Lemd2 can rescue the disease phenotype.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
Miao Cui, Ning Liu, Rhonda S. Bassel-Duby, Eric N. Olson
CIRCULATION RESEARCH
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
Peiheng Gan, Rhonda S. Bassel-Duby, Ning Liu, Eric N. Olson
CIRCULATION RESEARCH
(2022)
Meeting Abstract
Cardiac & Cardiovascular Systems
Takahiko Nishiyama, Yu Zhang, John Mcanally, Lin Xu, Ning Liu, Rhonda S. Bassel-Duby, Eric N. Olson
Meeting Abstract
Cardiac & Cardiovascular Systems
N. M. Stege, T. R. Eijgenraam, V. Oliveira Nunes Teixeira, A. M. Feringa, E. M. Schouten, C. A. Makarewich, R. Bassel-Duby, E. N. Olson, R. A. De Boer, H. H. W. Sillje
EUROPEAN JOURNAL OF HEART FAILURE
(2022)
Meeting Abstract
Transplantation
Tobias Bohnenpoll, Eric Olson, Mykola Dergai, Jennifer Cox, Simone Romoli, I-Ju Lo, Johannes Pospiech, Krishan Vishnolia, Mark Mcconnell, Marvin Gunawan, Michaela Bayerlova, Nicolette Honson, Niklas Michel, Nikolas Stroth, Olivier Radresa, Philipp Skroblin, Priyanka Kohli, Seamus Ragan, Shenshen Lai, Steven Bromidge, David Powell, Uwe Andag, Andrew King
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2022)
Meeting Abstract
Transplantation
Eric Olson, Mark Mcconnell, Seamus Ragan, Jennifer Cox, Jeff Lester, Charles Nieh, Jay Kuo, Andrew King
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2022)
Article
Medicine, Research & Experimental
Andres Ramirez-Martinez, Yichi Zhang, Marie-Jose Van den Boogaard, John R. McAnally, Cristina Rodriguez-Caycedo, Andreas C. Chai, Francesco Chemello, Maarten P. G. Massink, Inge Cuppen, Martin G. Elferink, Robert J. J. van Es, Nard G. Janssen, Linda P. A. M. Walraven-van Oijen, Ning Liu, Rhonda Bassel-Duby, Richard H. van Jaarsveld, Eric N. Olson
Summary: The study identifies a variant of the MYMX gene that is associated with CFZS-like abnormalities, leading to impaired fusion activity. Patient-derived cells and mouse models confirm the pathogenic role of this variant and CRISPR/Cas9 gene editing shows potential for therapeutic intervention.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
