Article
Chemistry, Applied
Reza Mahjub, Omid Heydari Shayesteh, Katayoun Derakhshandeh, Akram Ranjbar, Fereshteh Mehri, Ali Heshmati
Summary: In this study, a novel colorimetric bioassay method was developed for the sensitive determination of tobramycin. The method utilizes aptamers and silver nanoparticles to induce aggregation and visible color change in the presence of tobramycin, allowing for quantitative analysis. This method offers high sensitivity and visual detection.
Review
Biotechnology & Applied Microbiology
Wenfei Guo, Chuanxiang Zhang, Tingting Ma, Xueying Liu, Zhu Chen, Song Li, Yan Deng
Summary: This paper reviews the seriousness of heavy metal pollution and the application of aptasensors in detecting heavy metal ions. It focuses on the methods for selecting aptamers with high specificity and high affinity, as well as the construction methods and research progress of aptamers based on sensors. Finally, the challenges and future opportunities of aptasensors in detecting heavy metal ions are discussed.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Chemistry, Analytical
Wenfei Guo, Changchun Hu, Shuo Li, Dan Wei, Jie Zhou, Xueying Liu, Hui Chen, Song Li, Yan Deng
Summary: An aptamer-based electrochemical sensor was developed for detecting methyl parathion (MP). The MP aptamer (MPapta-6) with high affinity for MP was obtained using the modified magnetic beads-systematic evolution of ligands by enrichment (MB-SELEX). The constructed sensor showed a linear range of 1-105 pM with a low detection limit (LOD) of 0.49 pM. The sensor was successfully applied to detect MP in water samples with a recovery rate of 96.6%-103.5%.
ANALYTICA CHIMICA ACTA
(2023)
Review
Chemistry, Analytical
Jidong Tang, Xiaoqi Tao
Summary: This paper provides a summary of the research on AuNP-based aptasensors for detecting kanamycin in food products. The study reveals that the nonspecific adsorption of kanamycin on AuNPs can negatively impact the accuracy of detection. Therefore, it is necessary to reconsider the guiding principles for future research on aptasensors and provide improvement methods to avoid nonspecific adsorption.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Dmitry Gribanyov, Gleb Zhdanov, Andrei Olenin, Georgii Lisichkin, Alexandra Gambaryan, Vladimir Kukushkin, Elena Zavyalova
Summary: The development of sensitive techniques, such as Surface-enhanced Raman spectroscopy (SERS) and DNA aptamers, shows promise for combining rapid specific detection and quantitative determination of viruses, addressing the limitations of existing SERS-based aptasensors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Jiaming Tang, Qiming Kou, Xingyue Chen, Yarong Wang, Lulan Yang, Xu Wen, Xiaoling Zheng, Xueling Yan, Tao Le
Summary: An aptasensor based on silica nanoparticles was developed for the rapid detection of sulfadiazine (SDZ) residues in food. The aptasensor exhibited a wide dynamic linear range and a low limit of detection, and showed good recovery efficiency and reliability. The study suggests that the aptasensor has potential application value for the sensitive and rapid detection of SDZ residues in edible tissue.
ARABIAN JOURNAL OF CHEMISTRY
(2022)
Article
Chemistry, Inorganic & Nuclear
Heshmat Allah Samimi, Zeinab Saberi
Summary: A biosensor based on fluorescence quenching and ssDNA recognition tool was developed for Pb2+ detection. The aptasensor showed a linear range of 0.5 to 300 nM and a detection limit of 0.2 nM. Satisfactory results were obtained in monitoring Pb2+ levels in real samples.
Article
Chemistry, Analytical
Hailing Cao, Jingxuan Xie, Jiayi Cheng, Yanan Xu, Xing Lu, Jie Tang, Xiaojie Zhang, Houyu Wang
Summary: In this study, a CRISPR Cas12a-powered silicon surface-enhanced Raman spectroscopy (SERS) ratiometric chip was developed for sensitive and reliable quantification of CRISPR without preamplification. The chip demonstrated ultrahigh sensitivity and good reproducibility in the detection of PDGF-BB, showing potential for future applications.
ANALYTICAL CHEMISTRY
(2023)
Review
Chemistry, Analytical
Beibei Lou, Yanfei Liu, Meilin Shi, Jun Chen, Ke Li, Yifu Tan, Liwei Chen, Yuwei Wu, Ting Wang, Xiaoqin Liu, Ting Jiang, Dongming Peng, Zhenbao Liu
Summary: Viruses pose a serious threat to human life and health. Accurate early diagnosis is crucial for controlling and treating virus infections. In this review, the application of aptasensors for virus detection in recent years is summarized. The common detection mechanisms and the use of aptamers are illustrated. The relationships between virus biomarkers, detection methods, and their performance are revealed. Furthermore, the challenges and future directions of aptasensors are discussed.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Review
Chemistry, Analytical
Xiaoyi Xu, Hua Min, Ying Li
Summary: Ratiometric fluorescent probes based on carbon quantum dots (CQDs) and rare earth ions (RE3+) have attracted widespread attention for their applications in various fields. This review summarizes the combination of CQDs with rare earth complexes, the synthesis strategies of RE-CQD hybrids, and the important applications of RE-CQD fluorescent probes in public safety sensing, chemical sensing, biomedical sensing, temperature sensing, and pH sensing.
ANALYTICAL METHODS
(2023)
Article
Biophysics
Farzaneh Asadpour, Mohammad Mazloum-Ardakani, Farzaneh Hoseynidokht, Seyed Mohammad Moshtaghioun
Summary: An innovative label-free electrochemical aptasensing platform was developed for insulin detection using functionalized mesoporous silica thin-film coated on a glassy carbon electrode through electrochemically assisted self-assembly. This platform offers sensitive and rapid detection of insulin without the need for cargo as an electrochemical marker at low cost and with a fast modification time.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Spectroscopy
Maryam Shirani, Heibatullah Kalantari, Mohammad Javad Khodayar, Maryam Kouchak, Nadereh Rahbar
Summary: A novel nanobiosensor was developed using aptamer and gold nanoparticles conjugate in poly vinyl alcohol hydrogel for sensitive detection of digoxin in human plasma samples. The sensor performance was optimized and linear calibration curves were obtained for both external and plasma samples, with detection limits of 2.9 and 3.1 ng L-1, respectively. The nanofluoroprobe showed good reproducibility and was able to detect digoxin in spiked plasma samples without pretreatment.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Materials Science, Multidisciplinary
Xiao Wei, Shen Zhang, Tong Wang, Aixia Chen, Jifeng Guo, Bo Bai, Shengke Yang, Yaqian Li, Xinyi Wang, Xinni Liu
Summary: This study reported a fluorescent sensor that combines luminescent CsPbBr3 quantum dots and molecularly imprinted polymers for trace tetracycline detection in aqueous environments. The sensor showed good linear correlation and low detection limit under optimal conditions. The results pave the way for the practical application of IPQDs in detection environments.
JOURNAL OF MATERIALS CHEMISTRY C
(2022)
Review
Biochemical Research Methods
Farahnaz Behzad, Azam Samadi, Elaheh Rahimpour, Mahdi Adabi, Abolghasem Jouyban
Summary: Aptasensors have gained popularity due to their excellent particularity, selectivity, and responsiveness. When combined with gold nanoparticles (AuNPs), these biosensors can detect various chemical and biological molecules. The changing color of AuNPs provides a simple explanation for the sensor's response. This review summarizes the recent developments in AuNP-based colorimetric aptasensors for sensing different analytes, discusses their uses, challenges, and prospects for forming new aptasensors.
Review
Environmental Sciences
Kamal Rahimizadeh, Qurat ul ain Zahra, Suxiang Chen, Bao T. Le, Ismat Ullah, Rakesh N. Veedu
Summary: Given the importance of public health, the development of quick, targeted, highly sensitive, and accurate technologies to monitor pathogenic microbes is crucial. Nanomaterial-conjugated aptasensors have advantages in improving sensitivity and specificity, and have potential applications in bioanalysis of food and environmental samples.
ENVIRONMENTAL RESEARCH
(2023)
Article
Food Science & Technology
Samet Ucak, Mediha Nur Zafer Yurt, Behiye Busra Tasbasi, Elif Esma Acar, Osman Altunbas, Ali Soyucok, Ali Aydin, Veli Cengiz Ozalp, Mert Sudagidan
Summary: Metagenomic analysis revealed the bacterial microbiota of Boza samples, with a dominance of Lactococcus and Enterococcus after pre-enrichment. Clonal relationships, virulence properties, and antibiotic resistance of Enterococcus faecium strains in Boza were determined, highlighting the importance of microbial dynamics in fermentation.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Environmental Sciences
Elif Esra Altuner, Veli Cengiz Ozalp, M. Deniz Yilmaz, Muhammed Bekmezci, Fatih Sen
Summary: This study evaluated the analytical studies of Chitosan-Cobalt(II) nanoparticles and developed a selective TMB biosensor using catalytic reaction. The synthesized nanoparticles showed similar behavior to the peroxidase enzyme.
Article
Immunology
Mert Sudagidan, Veli Cengiz Ozalp, Ozge Can, Hakan Eligul, Mediha Nur Zafer Yurt, Behiye Busra Tasbasi, Elif Esma Acar, Murat Kavruk, Oner Kocak
Summary: This study investigated the surface microbiota of houseflies and identified the presence of Staphylococcus, Bacillus, and Enterococcus. Staphylococcus strains were selectively isolated and found to carry virulence genes and exhibit antibiotic resistance. Biofilm formation was observed in some strains, which played an important role in bacterial transmission on the surface of houseflies. The study sheds light on the composition and carriage of pathogenic staphylococci on housefly surfaces.
MICROBIAL PATHOGENESIS
(2022)
Article
Chemistry, Analytical
Ali D. Dursun, Baris A. Borsa, Gulay Bayramoglu, M. Yakup Arica, Veli C. Ozalp
Summary: A Surface Plasmon Resonance (SPR) aptasensor was developed for the sensitive detection of Brucella melitensis in milk samples. By using aptamers with high affinity and specificity, the aptasensor enables quick and efficient purification and detection of Brucella melitensis contamination in milk samples. The integration of a magnetic isolation step and SPR technology allows for instant and reliable detection of Brucella melitensis.
Article
Nanoscience & Nanotechnology
Onur Bulut, M. Deniz Yilmaz
Summary: In this study, a facile and convenient method was presented for the preparation of silver nanoparticles using quercetagetin as a reducing and stabilizing agent. The results showed that these silver nanoparticles exhibited excellent catalytic activity and could efficiently degrade a dye within a short period of time. Additionally, the silver nanoparticles demonstrated cytotoxic activity.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Analytical
Bilge Guvenc Tuna, Dilara Buse Durdabak, Meltem Kazak Ercan, Soner Dogan, Murat Kavruk, Ali Dogan Dursun, Serap Demir Tekol, Caner Celik, Veli Cengiz Ozalp
Summary: This study presents a direct and fast detection method for the CoV-2 genome in nose-throat swab samples, without the need for further processing. The method utilizes fluorescein-loaded mesoporous silica nanoparticles with specific gene sequence probes on their surface, which are released upon hybridization with the target viral genome.
Article
Biochemistry & Molecular Biology
Esra Ersoy Omeroglu, Dilhun Keriman Arserim-Ucar, Zeynep Yegin, Nevzat Caglayan, Mediha Nur Zafer Yurt, Behiye Busra Tasbasi, Elif Esma Acar, Samet Ucak, Veli Cengiz Ozalp, Mert Sudagidan
Summary: PCR amplification and next-generation sequencing were utilized to identify the bacterial communities in propolis samples collected from Apis mellifera production hives in Eastern Turkiye. Firmicutes were found to be the dominant phylum, followed by Proteobacteria, Actinobacteria, Tenericutes, and Spirochaetes. Bacillaceae, Enterobacteriaceae, and Enterococcaceae were the top three bacterial families, with Bacillus being the dominant genus. Further investigation is needed to understand the microbiota of propolis and its potential applications from ecological, nutritional, and medicinal perspectives.
CHEMISTRY & BIODIVERSITY
(2023)
Article
Spectroscopy
M. Deniz Yilmaz, Haluk Samet Kocak, Hatice Kubra Kara
Summary: A novel long wavelength monostyryl BODIPY derivative BDZ-1 was synthesized and successfully used for the high sensitivity and selectivity detection of oxalyl chloride, a highly reactive and harmful chemical. The color and fluorescence changes of BDZ-1 solution allow accurate detection of oxalyl chloride, and the practicability of BDZ-1 was further demonstrated by using a smartphone application for on-site detection.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Food Science & Technology
Ali Aydin, Mert Sudagidan, Zhanylbubu Mamatova, Mediha Nur Zafer Yurt, Veli Cengiz Ozalp, Jacob Zornu, Saraya Tavornpanich, Edgar Brun
Summary: In this study, the presence of Pseudomonas in the skin swabs of seabass farmed in the Aegean Sea was analyzed and identified. A total of 46 viable Pseudomonas strains were isolated using conventional methods. Antibiotic susceptibility testing revealed that three strains were resistant to doripenem and two strains were resistant to imipenem. This data provides insight into the bacterial diversity and antibiotic resistance of psychrotrophic Pseudomonas in seabass from the Aegean Sea in Turkey.
Article
Chemistry, Multidisciplinary
Sinem Altinisik, Hani Zeidan, M. Deniz Yilmaz, Mustafa E. Marti
Summary: Betaine from natural sources is still preferred over its synthetic analogue in secondary industries. In this study, reactive extraction of betaine from sugarbeet industry byproducts was investigated. Dinonylnaphthalenedisulfonic acid (DNNDSA) was used as the extraction agent, and various factors affecting betaine extraction were discussed. The results showed that reactive extraction has great potential for betaine recovery due to its high efficiency, simplicity, low energy demand, and cost.
Article
Nanoscience & Nanotechnology
Haluk Samet Kocak, Onur Bulut, M. Deniz Yilmaz
Summary: A new dicationic BODIPY-based fluorescent amphiphile with broad-spectrum antibacterial activity against both Gram-positive and Gram-negative bacteria has been synthesized. It exhibits stronger antimicrobial activity compared to benzalkonium chloride (BAC), a structurally similar disinfectant. The compound also shows antibiofilm activity against Staphylococcus epidermidis with a low minimum biofilm eradication concentration. Its interaction with bacterial cell and genomic DNA was further evaluated to understand its antibacterial mechanism.
ACS APPLIED BIO MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Veli Cengiz Ozalp, Samet Ucak, Ali D. Dursun, Mert Sudagidan, Oyku Icin, Cekdar Vakifahmetoglu, Laura M. Henning, Ulla Simon, Aleksander Gurlo
Summary: Different types of ordered mesoporous silica (OMS) nanoparticles, including COK-12 and modified COK-12, have been used as drug carriers for controlled drug adsorption and release. Cytotoxicity analysis showed that regular COK-12 particles had lower harm to mammalian cultured cells compared to other OMS nanoparticles. Regular COK-12 was further used as a carrier for a dual antibiotic-loaded drug delivery material, which showed enhanced antibiotic treatments against methicillin-resistant Staphylococcus strains through aptamer targeting.
JOURNAL OF DRUG DELIVERY SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Ali Dogan Dursun, Soner Dogan, Murat Kavruk, B. Busra Tasbasi, Mert Sudagidan, M. Deniz Yilmaz, Bayram Yilmaz, Veli C. Ozalp, Bilge G. Tuna
Summary: A labelless biosensor based on surface plasmon resonance was developed to measure the concentration of Intercellular Adhesion Molecule-1 (ICAM-1) in blood samples. The sensor showed high specificity and low detection limit, providing real-time information on ICAM-1 protein concentration.