Article
Engineering, Environmental
Rocca C. Chin-On, Maria J. Barbosa, Rene H. Wijffels, Marcel Janssen
Summary: Microalgae are a promising renewable feedstock for various biobased products. To reduce production costs, a novel V-shaped photobioreactor is proposed to capture and dilute sunlight, thereby improving photosynthetic efficiencies and biomass productivities of microalgae.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Environmental Sciences
Angela Perales-Perez, Maria D. Macias-Sanchez, Jesus Ruiz, Jose A. Perales, Carmen Garrido-Perez
Summary: This study investigates the recycling of nutrients in a recirculating aquaculture system through a microalgae-bacteria consortium. It also explores the effectiveness of filtration in reducing solids concentrations in the effluent using a multi-layer sand column.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Microbiology
Jaimet Bucheli, Herculano Cella, Camila Nader, Carlos Yure B. Oliveira, Camila Lisarb V. Bastolla, Rafael Garcia Lopes, Gabriella do Vale Pereira, Joao Karam, Roberto Bianchini Derner
Summary: The study characterized the bacterial communities associated with different cultivation systems of the microalga Tetradesmus obliquus. Metagenomic analysis was performed on biomass samples collected from sequential cultivation in three different systems. The results identified 72 OTUs, with Brevundimonas and Porphyrobacter being the most abundant taxa. Different cultivation systems showed an increase in taxa richness and diversity in the super-intensive and intensive systems.
JOURNAL OF BASIC MICROBIOLOGY
(2023)
Article
Cell Biology
Song Gao, Hongxiang Yan, Nathan Beirne, Mark Wigmosta, Michael Huesemann
Summary: This article presents a pond operation optimization tool that predicts biomass growth based on future weather conditions, in order to determine the optimal dilution rate for maximizing biomass productivity. Experimental results show that the weather-forecast-informed dilution method significantly improves biomass productivity compared to other dilution scenarios.
Review
Environmental Sciences
Lisa Aditya, T. M. Indra Mahlia, Luong N. Nguyen, Hang P. Vu, Long D. Nghiem
Summary: The review discusses the diversity of microalgae and bacteria and their potential as a consortium for efficient wastewater treatment and nutrient recovery. It emphasizes the use of microalgae biomass derived from wastewater as a renewable feedstock for various applications, and highlights the interactions and characteristics of microalgae in a consortium system.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Article
Environmental Sciences
Yue Zhao, Run Zhang, Liandong Jing, Wenjing Wang
Summary: This study evaluated the feasibility and durability of basalt fiber as a new carrier of periphyton and tracked the development process of basalt fiber-periphtyon (BFP) through bacterial community succession and physiological indicators. The results showed that basalt fiber was a suitable and durable carrier, and the periphyton could adapt to and grow abundantly on the basalt fiber, efficiently removing nutrients from water. The study also identified biomarkers for the development of BFP.
Review
Environmental Sciences
Alireza Fallahi, Fariba Rezvani, Hashem Asgharnejad, Ehsan Khorshidi Nazloo, Nima Hajinajaf, Brendan Higgins
Summary: Nitrogen and phosphorus pollution can lead to eutrophication which disrupts ecosystems. Wastewater treatment using microalgae-bacteria consortia can enhance nutrient removal efficiency. Understanding the mechanisms of interaction between microalgae and bacteria can improve nutrient transformation and recovery, with the nitrogen source in wastewater playing a crucial role in their interaction.
Article
Plant Sciences
Jan Helge Behr, Ioannis D. Kampouris, Doreen Babin, Loreen Sommermann, Davide Francioli, Theresa Kuhl-Nagel, Soumitra Paul Chowdhury, Joerg Geistlinger, Kornelia Smalla, Guenter Neumann, Rita Grosch
Summary: This study investigated the establishment of a microbial consortium in the rhizosphere of winter crops and its effect on plant performance under different farming practices. The results showed that the microbial consortium significantly improved plant nutrient status and performance, especially under organic farming.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biology
Simon Rosenau, Elisa Oertel, Alexander Charles Mott, Jens Tetens
Summary: This study conducted a 10-week feeding experiment on 120 African catfish and analyzed the intestinal microbiota, finding that spirulina supplementation may lead to an increase in bacterial richness, but the overall microbial community structure was not affected.
Article
Biotechnology & Applied Microbiology
Thi-Dieu-Hien Vo, Mai-Duy-Thong Pham, Bao-Trong Dang, Cong-Sac Tran, Thanh-Son Le, Van-Truc Nguyen, Thanh-Binh Nguyen, Chitsan Lin, Sunita Varjani, Thanh-Son Dao, Trong-Vinh Bui, Ky-Phuong-Ha Huynh, Xuan-Thanh Bui
Summary: This study investigated the effects of nitrogen species and biomass retention time on nutrient uptake and biomass productivity in Chlorella sp. It was found that 100% NO3--N promoted algae growth but had lower nutrient removal rates compared to 100% NH4+-N. The presence of ammonia nitrogen led to the flocculation of microalgae rather than biomass production. In a continuous membrane photobioreactor, shorter biomass retention time resulted in higher biomass productivity and pollutant removal rates.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2022)
Article
Agricultural Engineering
Yue Wang, Yuanyuan He, Xiaoqiang Li, Dillirani Nagarajan, Jo-Shu Chang
Summary: A novel microalgae-bacteria consortium was developed for effective degradation of Chlortetracycline (CTC) at concentrations up to 80 mg/L. The consortium exhibited resistance to CTC and utilized biosorption and extracellular enzymes for CTC removal. Shifts in microbial community composition were observed in the presence of CTC, with specific bacterial genera becoming dominant.
BIORESOURCE TECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Andrea Hidalgo-Arias, Victor Munoz-Hisado, Pilar Valles, Adelina Geyer, Eva Garcia-Lopez, Cristina Cid
Summary: Endolithic microorganisms living in the cracks of rocks in Deception Island, Antarctica exhibit diverse adaptations to extreme environmental conditions and show potential significance in astrobiology research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Plant Sciences
Chakrit Bunyoo, Peerapat Roongsattham, Sirikorn Khumwan, Juthaporn Phonmakham, Passorn Wonnapinij, Arinthip Thamchaipenet
Summary: This study investigated the structure and diversity of duckweed-associated bacteria (DAB) among four duckweed subtypes under natural and nutrient-deficient conditions using 16S rRNA amplicon sequencing. The results showed that Proteobacteria was the dominant phylum in duckweed samples. A total of 24 microbial genera were identified as a core microbiome, with consistent proportions across all duckweed subtypes. The diversity of microbial communities decreased after nutrient-deficient stress, but the relative abundance of certain genera significantly increased. Functional prediction of the metagenome data revealed enrichment of essential pathways involved in DAB colonization and biodegradation under stress condition.
Article
Biotechnology & Applied Microbiology
G. I. Romero-Villegas, V. A. Burboa-Charis, E. Navarro-Lopez, M. C. Ceron-Garcia, F. G. Acien-Fernandez, M. I. Estrada-Alvarado, N. P. Rout, L. A. Cira-Chavez
Summary: This study demonstrates that Chlorella sorokiniana can produce biomass using centrate from municipal wastewater as the sole nutrient source, with the highest biomass yield achieved at 35 degrees Celsius. The microalgal strain showed tolerance to excess ammonium and achieved high productivity levels.
JOURNAL OF APPLIED PHYCOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Josivaldo Satiro, Andre Cunha, Ana P. Gomes, Rogerio Simoes, Antonio Albuquerque
Summary: The symbiosis of microalgae and bacteria is a promising alternative for industrial wastewater treatment. The impact of different inoculation ratios of microalgae and bacteria on the removal of organic matter, nitrogen compounds, phosphorus, and biomass formation was investigated. The results showed that with the appropriate inoculation ratio, the microalgae-bacteria consortium was able to effectively remove COD and ammoniacal nitrogen, and achieve higher biomass production.
APPLIED SCIENCES-BASEL
(2022)
Article
Environmental Sciences
Rebeca Lopez-Serna, Silvia Bolado, Ruben Irusta, Juan J. Jimenez
Summary: This study presents the concentration data of veterinary drugs in microalgae biomass collected from photobioreactors fed with piggery wastewaters for the first time. A comprehensive sample preparation method is recommended to monitor all intended analytes, and a linear relationship between the concentrations measured in biomass and effluent has been observed for most of the drugs.
Article
Chemistry, Multidisciplinary
David Moldes, Elena M. Rojo, Silvia Bolado, Pedro A. Garcia-Encina, Bibiana Comesana-Gandara
Summary: The world is facing a growing demand for protein, and new sustainable protein sources are needed due to environmental concerns associated with animal protein. Microalgae are considered as a high-quality protein source, but separating proteins from microalgae biomass and other compounds is a challenging process. Protein recovery from disrupted biomass often relies on organic solvents or harsh conditions, which are detrimental to protein stability and the environment. This review discusses different techniques and methods for protein recovery, including precipitation, filtration, chromatography, electrophoresis, and solvent extraction. Attention is given to the use of ionic liquids (ILs) and deep eutectic solvents (DESs) as promising solvents for efficient protein separation with reduced environmental costs compared to traditional alternatives. The review also examines various solvent recovery options for reusing the solvent and isolating the proteins.
APPLIED SCIENCES-BASEL
(2022)
Article
Agronomy
Alice Ferreira, Daniel Figueiredo, Rodrigo Cardeiras, Rui Nabais, Francisca Ferreira, Belina Ribeiro, Claudia M. D. S. Cordovil, F. Gabriel Acien, Luisa Gouveia
Summary: The overapplication of manure on agricultural soils leads to pollution of water resources, however, pretreatment methods such as photo-Fenton can make piggery wastewater suitable for microalgae growth.
Article
Environmental Sciences
Jorge Garcia-Marquez, Alba Galafat, Antonio Jesus Vizcaino, Andre Barany, Juan Antonio Martos-Sitcha, Juan Miguel Mancera, Gabriel Acien, Felix L. Figueroa, Francisco Javier Alarcon, Salvador Arijo, Roberto Teofilo Abdala-Diaz
Summary: This study evaluated the effects of moderate dietary supplementation with the green microalga Chlorella fusca on growth, metabolism, and digestive functionality in juvenile thick-lipped grey mullet. The results showed that fish fed with the microalgae-supplemented diet had improved growth performance, fatty acid profile, metabolic response, and increased intestinal digestive and absorptive capacity.
FRONTIERS IN MARINE SCIENCE
(2022)
Article
Food Science & Technology
Silvia Villaro, Ainoa Morillas-Espana, Gabriel Acien, Tomas Lafarga
Summary: The present study aimed to maximize the productivity of Arthrospira platensis BEA005B (Spirulina) by identifying the optimal combination of dilution rate and depth of the culture in raceway reactors. The optimal combination was determined to be a depth of 0.10 m and a dilution rate of 0.33 day(-1), resulting in a biomass productivity of 30.2 g.m(-2.)day(-1) on a dry weight basis. The composition of the produced biomass was found to be rich in proteins and had potential functional properties for the development of innovative and sustainable foods.
Article
Biology
Ainoa Morillas-Espana, Ruperto Bermejo, Roberto Abdala-Diaz, Angela Ruiz, Tomas Lafarga, Gabriel Acien, Jose Maria Fernandez-Sevilla
Summary: In this study, a biorefinery based on Oscillatoria sp. was developed to produce high-value compounds, such as C-phycocyanin and biostimulants. The study optimized the production of Oscillatoria biomass, extracted C-phycocyanin, evaluated its colorant capacity, confirmed its safety, and evaluated the economic feasibility. The results demonstrate the potential commercial applications of microalgae-related processes.
Article
Agricultural Engineering
Rebecca Nordio, Francisco Javier Delgado, Ana Sanchez-Zurano, Jose Gonzalez Hernandez, Enrique Rodriguez-Miranda, Jose Luis Guzman, Tomas Lafarga, Gabriel Acien
Summary: The present study aimed to evaluate the use of microalga Scenedesmus almeriensis for treating untreated urban wastewater. Two 12 m(3) raceway reactors, one supplemented with wastewater and the other with chemical fertilizer, were operated outdoors in a semi-continuous mode for eight months. The results showed that S. almeriensis could be produced in wastewater without affecting the photosynthetic apparatus, with an average productivity of 13 g center dot m(-2)center dot day(-1) in both systems. Moreover, the nutrient content of the wastewater, including nitrogen, phosphorous, and chemical oxygen demand, was reduced to meet European limitations, with average removal rates of 2.2, 0.2, and 3.0 g center dot m(-2)center dot day(-1) respectively. The raceway reactors demonstrated high potential for microalgal production and efficient biotreatment, proving to be robust and reliable. Additionally, an accurate model was developed to evaluate the effect of environmental conditions on biomass productivity in the clean system (R-2 = 0.9, p = 0.0002).
BIORESOURCE TECHNOLOGY
(2023)
Article
Environmental Sciences
Joseph Santhi Pechsiri, Jean-Baptiste E. Thomas, Naoufel El Bahraoui, Francisco Gabriel Acien Fernandez, Jamal Chaouki, Saad Chidami, Rodrigo Rivera Tinoco, Jose Pena Martin, Cintia Gomez, Michel Combe, Fredrik Grondahl
Summary: This study compares the environmental performance of conventional reactors and a proposed internally illuminated novel closed reactor design. The results show that the novel photobioreactor can significantly reduce impacts such as eutrophication and climate change when leveraging renewable energy sources and the photosynthesis process in urban-industrial symbiosis scenarios.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Biotechnology & Applied Microbiology
Elvira Navarro-Lopez, Juan Jose Gallardo-Rodriguez, Maria del Carmen Ceron-Garcia, Inmaculada Gallego-Lopez, Francisco Gabriel Acien-Fernandez, Emilio Molina-Grima
Summary: The effect of different parameters on the extraction of biostimulant molecules from microalgal biomass was investigated, and the optimal conditions for using organic green solvents (acetone or ethanol) were determined for maximum germination index.
JOURNAL OF APPLIED PHYCOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Jorge Garcia-Marquez, Jesus Vizcaino Antonio, Andre Barany, Alba Galafat, Gabriel Acien, Felix L. Figueroa, Javier Alarcon Francisco, Miguel Mancera Juan, Juan Antonio Martos-Sitcha, Salvador Arijo, Roberto Teofilo Abdala-Diaz
Summary: The study found that the combined administration of Chlorella fusca and ethanol-inactivated Vibrio proteolyticus significantly improved the growth performance and feed utilization of the thick-lipped grey mullet, while enhancing the lipid quality and n-3 polyunsaturated fatty acids. This has potential benefits for human nutrition.
Article
Microbiology
Jorge Garcia-Marquez, Rosa Maria Rico, Francisco Gabriel Acien, Juan Miguel Mancera, Felix L. Figueroa, Antonio Jesus Vizcaino, Francisco Javier Alarcon, Miguel Angel Morinigo, Roberto Teofilo Abdala-Diaz
Summary: This study investigates the effects of a blend of microalgae on Sparus aurata juveniles. The results show that the addition of microalgae tends to decrease body lipids and affects fatty acid profile. In particular, the diet containing 25% of the microalgae blend increases DHA levels. Additionally, this diet promotes appropriate microbial diversity and influences fatty acid composition and lipid metabolism in fish.
Article
Mathematics
Pablo Otalora, Jose Luis Guzman, Manuel Berenguel, Francisco Gabriel Acien
Summary: The industrial production of microalgae is a sustainable and interesting process, especially in terms of its applications in wastewater treatment. Neural network models have been developed to optimize the process and characterize the pH dynamics in different raceway reactors. These models are able to predict pH profiles using available measurable process data and demonstrate the potential of artificial neural networks in modeling continuous dynamic systems in the industry.
Article
Chemistry, Medicinal
Jorge Garcia-Marquez, Bruna Rodrigues Moreira, Piedad Valverde-Guillen, Sofia Latorre-Redoli, Candela T. Caneda-Santiago, Gabriel Acien, Eduardo Martinez-Manzanares, Manuel Mari-Beffa, Roberto T. Abdala-Diaz
Summary: This study investigated the cytotoxic activity of ulvan polysaccharides obtained from Ulva rigida against human cancer cell lines and zebrafish embryos. The results showed that ulvan had cytotoxic effects on human cancer cell lines, particularly on colon cancer cells. However, ulvan also had negative effects on the growth rate and osmolar balance of zebrafish embryos, indicating that its use should be limited to specific concentrations.
Article
Agriculture, Dairy & Animal Science
Jorge Garcia-Marquez, Daniel Alvarez-Torres, Isabel M. Cerezo, Marta Dominguez-Maqueda, Felix L. Figueroa, Francisco Javier Alarcon, Gabriel Acien, Eduardo Martinez-Manzanares, Roberto T. Abdala-Diaz, Julia Bejar, Salvador Arijo
Summary: This study assessed the impact of dietary green microalgae and ethanol-inactivated Vibrio proteolyticus on thick-lipped grey mullet juvenile fish. The results showed that the diet influenced the composition of intestinal microbiota without affecting its functionality. Additionally, the diet effectively regulated the transcription of stress and immune-related genes, enhancing the fish's resistance against stress and infections.
Article
Computer Science, Information Systems
M. Munoz, J. L. Guzman, M. Torres, F. G. Acien
Summary: This paper presents a solution for data management in large-scale microalgae cultivation plants based on Internet of Things (IoT) technology. The solution includes a cloud-based architecture and a graphical front-end for real-time and historical data access, as well as the management of different user profiles.