Article
Biochemistry & Molecular Biology
Maria C. C. Klaiss-Luna, Malgorzata Jemiola-Rzeminska, Kazimierz Strzalka, Marcela Manrique-Moreno
Summary: Host defense peptides are natural antimicrobial agents that have potential therapeutic use. LTX-315, a recently reported peptide, has shown promising oncolytic activity, but its interaction with membranes has not been well studied. This research investigated the interaction of LTX-315 with eukaryotic lipids using artificial systems, and found a strong interaction with phosphatidylserine. These findings contribute to the understanding and development of improved anticancer peptides.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Motoki Nishimura, Yoshimasa Kawaguchi, Kakeru Kuroki, Yuna Nakagawa, Toshihiro Masuda, Takayuki Sakai, Kenichi Kawano, Hisaaki Hirose, Miki Imanishi, Tomoka Takatani-Nakase, Sergii Afonin, Anne S. Ulrich, Shiroh Futaki
Summary: Spatiotemporal structural alterations in cellular membranes play a crucial role in many vital processes. The N-terminal helical segment (EpN18) of Epsin-1 protein is essential in inducing positive membrane curvature. Understanding the structural features of EpN18 can help design effective tools for controlling membrane curvature.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
David Gonzalez Bullon, Kepa B. Uribe, Jone Amuategi, Cesar Martin, Helena Ostolaza
Summary: Several toxins interact with cholesterol in different ways, affecting cellular permeabilization. Cholesterol enhances the lytic potency of ACT by promoting toxin oligomerization, a crucial step for membrane permeabilization. The mechanistic details by which cholesterol facilitates lytic pore formation remain largely unexplored.
Article
Biochemistry & Molecular Biology
Uxue Ballesteros, Asier Etxaniz, Marina N. Iriondo, Yaiza R. Varela, Melisa Lazaro, Ana R. Viguera, L. Ruth Montes, Mikel Valle, Felix M. Goni, Alicia Alonso
Summary: This study explores the binding of LC3C to lipid vesicles, bilayers, and monolayers, as well as the protein-dependent perturbing effects, in the absence of mitochondrial lipid cardiolipin. The results suggest that lipidated LC3C can induce vesicle aggregation, lipid mixing, and release of vesicular aqueous contents, supporting its role in autophagosome elongation and fusion.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Chemistry, Multidisciplinary
Takahiro Iwata, Hisaaki Hirose, Kentarou Sakamoto, Yusuke Hirai, Jan Vincent V. Arafiles, Misao Akishiba, Miki Imanishi, Shiroh Futaki
Summary: The Fc region binding peptide can assist in delivering immunoglobulin G into cells, forming particle-like liquid droplets; upon contact with the cellular membrane, the droplets spontaneously enter cells and distribute throughout; translocation of IgG is achieved through involvement of cellular machinery and membrane ruffling.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Physical
Minoru Nakano, Hiroyuki Nakao, Shigeharu Yoshida, Masakazu Fukuda, Manjiro Imai, Keisuke Ikeda
Summary: This study investigates the dynamic changes of lipids with membrane curvature, revealing that lipids in membranes with high positive curvature have unique packing properties, resulting in enhanced hydrophobic hydration and increased activation entropy. These findings provide important insights into the functions and structural changes of biomembranes.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2022)
Article
Chemistry, Physical
Daniela Ciumac, Haoning Gong, Richard A. Campbell, Mario Campana, Hai Xu, Jian R. Lu
Summary: Surface pressure-area isotherm analysis showed non-ideal mixing in all binary lipid systems, but it was thermodynamically favored. An increase in surface pressure leads to demixing, resulting in phase separation and cluster formation. Neutron reflectivity measurements indicated stronger binding and penetration of G4 to the DPPG/TMCL monolayer, suggesting greater affinity due to electrostatic interaction.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Mohammad ElGamacy, Birte Hernandez Alvarez
Summary: Natural helical bundles (HBs) are protein folds built of α helices that adopt diverse topologies and functions, ranging from structural scaffolds to molecular switches; Symmetric HBs, such as coiled coils, serve as ideal model systems for studying protein folding and design.
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Moumita Ghosh, Anna Majkowska, Rajkumar Mirsa, Santu Bera, Jose Carlos Rodriguez-Cabello, Alvaro Mata, Lihi Adler-Abramovich
Summary: This study demonstrates the temperature-independent formation of stable membranes by the spontaneous interaction of intrinsically disordered elastin-like polypeptides (ELPs) with short aromatic peptides, offering novel possibilities for controlling the conformational restriction of intrinsically disordered proteins and using them in the design of new materials. The membranes are stable over time and display durability over a wide range of environmental parameters, supporting preosteoblast cell adhesion and proliferation as well as pH-dependent cargo release. Simple noncovalent interactions with short aromatic peptides can overcome conformational restrictions due to the phase transition to facilitate the formation of complex bioactive scaffolds.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Cell Biology
Karis Amata Zecchi, Thomas Heimburg
Summary: Recent evidence suggests that lipid bilayers exhibit conductive properties but are commonly considered inert insulators. Under voltage-clamp conditions, lipid bilayers display current traces with discrete conduction-steps and outward rectification. The non-linear conduction in lipid membranes at different depths indicates voltage-gated and mechanosensitive channels.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Konstantin Pinigin, Timur R. Galimzyanov, Sergey A. Akimov
Summary: Cell membranes exhibit heterogeneity in lipid composition and can form nanoscopic liquid-ordered domains known as rafts. Antimicrobial amphipathic peptides may affect domain fusion, potentially leading to misregulation of raft clustering.
Review
Physics, Multidisciplinary
Teshani Kumarage, Nicholas B. Morris, Rana Ashkar
Summary: Lipid bilayers, the main component of cell membranes, possess unique properties that allow for both cellular protection and the diffusion of molecules necessary for biological functions. They have been applied in various practical applications such as artificial cells, drug nanocarriers, and biosensors. Understanding how lipid bilayers respond to additives is crucial for optimizing their performance in biological and synthetic systems.
FRONTIERS IN PHYSICS
(2023)
Article
Multidisciplinary Sciences
Brandy N. Curtis, Ellysa J. D. Vogt, Kevin S. Cannon, Amy S. Gladfelter
Summary: Most cells can sense and change their shape to carry out cell processes, with the septin cytoskeleton playing a vital role in coordinating shape changes. Septins assemble into higher-order structures and are found in regions of positive curvature on the cell membrane. Reconstituting the septin cytoskeleton in a cell-free system has allowed for the study of assembly mechanisms at high resolution. Understanding how septins assemble and sense curvature at different stages is crucial for understanding cell shape changes.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2022)
Article
Chemistry, Analytical
Madhurima Chattopadhyay, Hanna Orlikowska, Emilia Krok, Lukasz Piatkowski
Summary: Biological membranes are essential for cell functioning, with lipid self-assembly in aqueous media used to develop model systems mimicking the lipid bilayer structure for studying biological processes and developing applications. The hydration properties of lipid bilayers are crucial for cellular activity, particularly the local membrane dehydration in events like neurotransmission, fertilization, and viral entry. A new method has been introduced to quantify the hydration state of lipid bilayers, based on changes in lipid lateral diffusion and fluorescence recovery after photobleaching technique.
Review
Plant Sciences
Chloe Beziat, Yvon Jaillais
Summary: Biological membranes are highly dynamic and constantly exchange vesicles between different compartments of the cell. Recent advances in optical microscopy and lipid visualization allow the study of lipid lateral motion in plants. This review discusses the parameters influencing lipid diffusion and their effects on membrane domain formation. Controlled lipid diffusion plays crucial roles in cell signaling, development, and environmental interactions.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)