Article
Biophysics
Huan Zhao, Xin Du, Hui Dong, Delin Jin, Feng Tang, Qing Liu, Ping Wang, Lei Chen, Peiqing Zhao, Yueyun Li
Summary: The construction of a sandwich-type electrochemical immunosensor allowed for the detection of cardiac troponin I (CTnI) content in human blood, demonstrating important clinical significance in the sensitive and accurate diagnosis of acute myocardial infarction (AMI).
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biotechnology & Applied Microbiology
Linlin Cao, Sumei Lu, Chengjie Guo, Wenqiang Chen, Yinan Gao, Diwen Ye, Zejun Guo, Wanshan Ma
Summary: Dynamic monitoring of tumor markers is essential for the diagnosis of malignant tumors, evaluation of therapeutic effects, and prognosis analysis of cancer patients. This study utilized immunosensor technology to detect the digestive tract tumor marker CA242. PdAgPt nanocomposites, with great biocompatibility, electrical conductivity, and catalytic properties, were synthesized and loaded onto MoS2 to create a new nanocomposite. Optimizing the experimental conditions, the label-free CA242 electrochemical immunosensor showed a wide detection range and low detection limit. Additionally, the CA242 immunosensor demonstrated excellent repeatability, selectivity, and stability, and successfully detected CA242 in human serum samples. Therefore, the label-free electrochemical immunosensor constructed in this study holds great potential for the detection of clinical biomarkers.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Xinyi Huang, Juncong Miao, Jinglong Fang, Xiaoting Xu, Qin Wei, Wei Cao
Summary: In this study, an electrochemical immunosensor was used as a simple analytical method for ultra-sensitive detection of NSE in human serum. With Cu-MOFs-Au as the electrode sensing surface and Fc-L-Cys as the label of Ab2, a ratiometric electrochemical immunosensor for quantitative analysis of NSE was prepared. The immunosensor showed excellent performance in the concentration range of 1 pg/mL to 1 mu g/mL, with a detection limit of 0.011 pg/mL.
Article
Chemistry, Physical
Biao Wang, Qi Yan, Bawurejiang Duolihong, Xiangdong Ma, Xiaohong Xia
Summary: Constructing an electrochemical immunosensor with dual-signal detection is important for sensitive and accurate detection of prostate-specific antigen (PSA), and this research successfully developed a dual-signal electrochemical immunosensor with a wide detection linear range and extremely low detection limits, which can be used for PSA detection in human serum samples with good recovery.
APPLIED SURFACE SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Yaqi An, Sheying Dong, Hao Chen, Li Guan, Tinglin Huang
Summary: The integration of COF/CNT into MOF to construct a label-free immunosensor for specific cancer detection showed promising results with low detection limit, wide linear range, good stability and specificity.
BIOELECTROCHEMISTRY
(2022)
Article
Nanoscience & Nanotechnology
Huan Liang, Chunlan Chen, Jing Zeng, Min Zhou, Li Wang, Guobao Ning, Quanmei Duan, Rui Han, Huifang Liu, Hui Zhao, Can-Peng Li
Summary: A sensitive dual-signal sandwich-type electrochemical immunosensor was designed for NGAL detection, showing good analytical performance and potential applications in AKI diagnosis.
ACS APPLIED NANO MATERIALS
(2022)
Article
Chemistry, Analytical
Pushap Raj, Man Hwan Oh, Kyudong Han, Tae Yoon Lee
Summary: Developed a simple, label-free, and highly sensitive immunosensor for early diagnosis of bacterial pathogens using Au@Mo-2-PANI nanocomposite. Employed a self-assembled monolayer of mercaptopropionic acid on the gold nanoparticle surface for covalent immobilization of antibodies to minimize nonspecific adsorption of bacterial pathogens. The biosensor showed high selectivity and sensitivity with a low limit of detection, detecting E. coli within 30 min and demonstrating practical utility in urine samples.
Article
Biochemistry & Molecular Biology
Botao Wang, Baoshan He, Rui Guo, Qiang Jiao, Ying Liang, Jinshui Wang, Yong Liu, Wenjie Ren, Zhiguang Suo
Summary: A novel competitive-type electrochemical immunosensor based on square wave voltammetry (SWV) response was developed for quantitative detection of Aminohydantoin (AHD). Nanocomposites with good electrical conductivity were prepared as a signal amplification platform by growing Au nanoparticles on the surface of Ce-based metal-organic framework (Ce-MOF). The method showed excellent specificity and promising potential for practical applications.
BIOELECTROCHEMISTRY
(2021)
Article
Chemistry, Analytical
Xinyi Huang, Juncong Miao, Xiaoting Xu, Dongmei Cao, Lei Liu, Qin Wei, Wei Cao
Summary: By combining the i-t curve and DPV, a dual-mode electrochemical immunosensor was fabricated using CoSe2-GO-Au as the sensing substrate, enabling sensitive detection of CYFRA 21-1.
MICROCHEMICAL JOURNAL
(2022)
Article
Biophysics
Zhenjiao Shang, Tong Su, Dangqin Jin, Qin Xu, Xiaoya Hu, Yun Shu
Summary: An integrated electrochemical immunosensor based on PDMS/Au film and Fe-Co MOF was fabricated for ultrasensitive detection of AFP. The sensor showed high sensitivity, wide linear range, and accurate detection of clinical samples.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Analytical
Xuefang Gu, Kaiyue Wang, Shu Tian, Xinyi Shao, Jianguo Li, Anping Deng
Summary: In this study, a surface-enhanced Raman scattering (SERS)/electrochemical dual-signal readout immunosensor was developed for simultaneous detection of beta-adrenergic agonists salbutamol (SAL), ractopamine (RAC), and phenylethanolamine A (PA). The immunosensor exhibited high sensitivity, specificity, accuracy, and precision in detecting the target compounds. The immunosensor can be used as a platform for the determination of other small molecular compounds in biological, food, and environmental analytical fields.
Article
Chemistry, Analytical
Fengping Liu, Lianguo Geng, Fanggui Ye, Shulin Zhao
Summary: A dual-signal strategy using nanocomposites has potential in cancer biomarker determination. By preparing MnO@C nanocomposite and loading AuNPs, a sandwich-type aptasensor was constructed for dual-signal electrochemical sensing of cancer biomarkers. This technology enables sensitive and accurate measurements, which can be applied in early disease diagnosis.
Article
Chemistry, Analytical
Gul Naz, Mohamed A. Salem, Bharat Prasad Sharma, Saleh D. Mekkey, Razium Ali Soomro, Selcan Karakus, Zeinhom M. El-Bahy
Summary: A label-free electrochemical immunosensor based on MXene nanosheets and gold nanoparticles was developed for sensitive detection of the breast cancer biomarker CALR. The sensor showed excellent antifouling properties and high sensitivity in the low concentration range.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Biao Wang, Qi Yan, Bawurejiang Duolihong, Xiangdong Ma, Xiaohong Xia
Summary: We developed a dual-signal electrochemical immunosensor using CoTe2@CeO2 nanocomposites as an electrocatalytic label for the detection of prostate-specific antigen (PSA). Experimental results showed that this immunosensor had a wide linear response range and low detection limits for PSA, with good detection and self-calibration capability. Additionally, this novel immunosensor has the potential for detecting other biomarkers by altering the recognition components.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Yuan-Yuan Tan, Hong-Sheng Tan, Mingjun Liu, Shan-Shan Li
Summary: In order to prevent and diagnose carcinoembryonic antigen (CEA) at an early stage, it is necessary to develop more accurate detection methods. This study constructed a new sensitive electrochemical ratiometric immunosensor for CEA determination using chitosan-ferrocenecarboxaldehyde-gold nanospheres (ChiFc-Au) and reduced graphene oxide-Pd@Au nanocomposites-thionine (rGO-Pd@Au-Thi). The immunosensor utilized dual-signal output to generate two electrical signals (IFc and IThi) that were positively correlated with CEA concentration. Compared to other immunosensors, the proposed immunosensor exhibited a wider linear range and a lower detection limit, making it suitable for CEA measurement in human serum samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)