Nonmetallic SERS-based immunosensor byintegrating MoS2 nanoflower and nanosheet towards the direct serum detection of carbohydrate antigen 19-9

Recently, nonmetallic substrates have stimulated great interest in surface-enhanced Raman scattering (SERS) -based immunoassay owing to their good uniformity, stability, and biocompatibility. In this context, a novel nonmetallic SERS-based immunoassay mediated by two-dimensional molybdenum disulfide (MoS2) was deliv-ered for the sensitive and specific monitoring of carbohydrate antigen 19-9 (CA19-9). The effective enrichment of molecules on the large active surfaces of MoS2 as well as potential 532-nm laser-induced charge transfer resonances between them engendered desirable enhancement factor values at the level of 105. Intriguingly, a sandwich immunocomplex combined MoS2 nanoflower and nanosheet exhibited not only a wide linear range from 5 x 10(-4) to 1 x 10(2) IU.mL(-1) but also a limit of detection as low as 3.43 x 10(-4) IU.mL(-1) towards CA19-9. More meaningful, the analytical result for clinical patient serum sample was basically compared with the con-ventional chemiluminescent immunoassay. Such a novel nonmetallic SERS-based immunosensor with desirable biocompatibility and sensitivity is promising for clinical diagnosis.

Automated summary

Recently, nonmetallic substrates have attracted great interest in SERS-based immunoassay due to their uniformity, stability, and biocompatibility. A novel nonmetallic SERS-based immunoassay mediated by two-dimensional molybdenum disulfide (MoS2) has been developed for sensitive and specific monitoring of CA19-9, showing potential for clinical diagnosis.