Preconcentrations of Zn(II) and Hg(II) in Environmental and Food Samples by SPE on B. licheniformis Loaded Amberlite XAD-4

In this work, the separations and preconcentrations of Zn(II) and Hg(II) ions on Bacillus lichenifoemis loaded onto Amberlite XAD-4 resin by solid-phase extraction has been performed. The biosorbent was characterized by using FT-IR, SEM, and EDX. pH, sample flow rate, eluent type and concentration, amount of B. licheniformis and XAD-4 resin, sample volume, and possible interfering ions effect were investigated in details as experimental variables in the SPE procedure. Limit of detection values for Zn(II) and Hg(II) were detected as 0.03 and 0.06 ng-mL(-1), respectively. 0.2-15 ng-mL(-1) linear range values were achieved for Zn(II) and Hg(II), respectively. Relative standard deviation values were found to be lower than 5%. For validation of the procedure, the certified standard reference materials (CWW-TM-D, EU-L-2, NCS ZC73O14, NCS ZC73350) were analyzed. The concentrations of Zn(II) and Hg( II) in water and food samples were measured by ICP-OES. Consequently, it can be inferred that the immobilized B. licheniformis microcolumn has ideal selectivity for Zn(II) and Hg(II) biosorption.

Automated summary

In this study, the separations and preconcentrations of Zn(II) and Hg(II) ions using Bacillus licheniformis on Amberlite XAD-4 resin by solid-phase extraction were performed. The biosorbent showed ideal selectivity for Zn(II) and Hg(II) biosorption with detection limits of 0.03 and 0.06 ng-mL(-1) respectively. The method demonstrated good sensitivity and selectivity in sample pre-treatment.