Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Volume 1863, Issue 12, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.bbamem.2021.183759
Keywords
Photomelittin; Melittin; Membrane active peptide; Peptide synthesis; Photoactive
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Funding
- Seton Hall University
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Photomelittin, a newly synthesized membrane active peptide, demonstrates light-dependent activity by substituting a specific amino acid derivative, showing promising applications in medicine, drug delivery, and biotechnological fields.
In this article we present the synthesis and characterization of a new form of the membrane active peptide melittin: photomelittin. This peptide was created by substituting the proline residue in melittin for a synthetic azobenzene amino acid derivative. This azobenzene altered the membrane activity of the peptide while retaining much of the secondary structure. Furthermore, the peptide demonstrates added light-dependent activity in leakage assays. There is a 1.5-fold increase in activity when exposed to UV light as opposed to visible light. The peptides further exhibit light-dependent hemolytic activity against human red blood cells. This will enable future studies optimizing photomelittin and other azobenzene-containing membrane active peptides for uses in medicine, drug delivery, and other biotechnological applications.
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